Recovery from gastric mucus depletion in the intact guinea pig mucosa

Schreiber, S.; Nguyen, Tien Hoa; Konradt, Manuela; Scheid, P.

doi:10.1080/00365520310006333

Cited by 4 publications

(7 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pH values and membrane potentials measured by the ultrafine double-barreled microelectrodes gave signals that were consistent with those that we reported earlier for the various structures in the secreting corpus gland (Schreiber et al 2003). Thus, the intracellular pH values of the secreting parietal cell were between 7.4 and 7.5, values unique to the gastric epithelium, whereas mucus-producing cells and chief cells in the gland were characterized by intracellular pH values of 7.2.…”

Section: Ph and Voltage Measurementsupporting

confidence: 72%

“…The explant of the corpus mucosa with stripped serosal surface (Rutten and Ito 1983;Schreiber and Scheid 1997;Schreiber et al 2003) has the advantage of a still intact mucosa, whereas the motility of the gastric wall is diminished by the destruction of the seromuscularis. Long-lasting acid secretion can be supported by the addition of the perfluorocarbon oxygen carrier, which has been successfully used as a gas carrier in medicine and biotechnology (Lowe 1997).…”

Section: Critique Of Methodsmentioning

confidence: 99%

“…The preparation of the gastric mucosa explant from guinea pig has previously been described in detail (Schreiber and Scheid 1997;Schreiber et al 2000Schreiber et al , 2003. The mucosae of male guinea pigs weighing 400-700 g were used.…”

Section: Gastric Mucosa Explant From Guinea Pigmentioning

confidence: 99%

“…The tip was positioned at the luminal surface of the mucus layer and then advanced in steps of 10 μm until it reached the neck cells of the glands. These cells are characterized by the small differences in their membrane potentials, in contrast to the uniform (gap-junction coupled) surface cells (Schreiber et al 2003). In the depth of the secreting gland cells, the step size was reduced to 2 μm.…”

Section: Measurements Of Phmentioning

confidence: 99%

See 3 more Smart Citations

In situ measurement of pH in the secreting canaliculus of the gastric parietal cell and adjacent structures

et al. 2007

View full text Add to dashboard Cite

The gastric H(+)/K(+)-ATPase is located within an infolding (secretory canaliculus) of the apical plasma membrane of gastric parietal cells. Our aim was to measure the pH values in the cytosol and canaliculus of the acid-secreting parietal cell and the adjacent gland lumen in situ. We used ultrafine double-barreled tip-sealed microelectrodes at high acceleration rates for intracellular and canalicular measurements. Immunohistochemical staining of the parietal cells was used to identify the track of the electrode and to estimate the position of the electrode tip at the time of the last intracellular measurement. En route to the deepest regions of the mucosa, where the average gland lumen pH was approximately 3, and on advancing in steps of 2 mum, the electrode entered the cytosol of the parietal cells, where the pH value was 7.4. Advancing the electrode further resulted, in several instances, in a sharp decrease in pH to an average value of 1.7 +/- 0.2, which we interpreted as the measurement within the canaliculus. When the electrode was advanced even further, the pH reading returned to the cytosolic value. From the difference in pH between the secreting canaliculus and the adjacent gland lumen, we concluded that the released acid was immediately buffered. Thus, the only cellular structure directly exposed to the highly acidic canalicular content is the apical membrane forming the canaliculus in the parietal cell.

show abstract

Section: Ph and Voltage Measurementsupporting

confidence: 72%

Section: Critique Of Methodsmentioning

confidence: 99%

Section: Gastric Mucosa Explant From Guinea Pigmentioning

confidence: 99%

Section: Measurements Of Phmentioning

confidence: 99%

See 2 more Smart Citations

In situ measurement of pH in the secreting canaliculus of the gastric parietal cell and adjacent structures

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Continuing secretion of mucins and shedding into the lumen lead to a rapid regeneration of the viscous mucus (Schreiber and Scheid, 1997). After experimental mucus depletion, a quarter of the gastric mucus layer was reconstituted within 5 min in the guinea pig model (Schreiber et al, 2003). This rapid turnover of the gastric mucus may suggest that H. pylori bacteria would be subjected to a permanent elution from the gastric mucosa and this might inevitably prevent a lifelong colonization of the stomach in the absence of adhesive structures.…”

Section: Introductionmentioning

confidence: 98%