Recovery of a Lassa-Related Arenavirus in Zimbabwe

Johnson, Karl M.; Taylor, P.; Elliott, Luanne H.; Tomori, Oyewale

doi:10.4269/ajtmh.1981.30.1291

Cited by 48 publications

(23 citation statements)

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“…The prototype, lymphocytic choriomeningitis (LCM) virus, is distributed almost world-wide, but of the remainder the Tacaribe complex is confined to the New World (mainly South America), Lassa virus is found in West Africa and viruses closely related to it have been isolated in Mozambique and Zimbabwe (Wulff et al, 1977;Johnson et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Structural and Cell-associated Proteins of Lassa Virus

Clegg

Lloyd

1983

Journal of General Virology

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Section: Introductionmentioning

confidence: 99%

Structural and Cell-associated Proteins of Lassa Virus

Clegg

Lloyd

1983

Journal of General Virology

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“…Antigen for the IFA test was prepared by methods previously described [5,14] in E-6 cells (a clone of Vero cells kept at the Centers for Disease Control). A radioimmunologicassay (Rl A) [ 15] was used toconfirm many of the serologic tests.…”

Section: Serologic Testsmentioning

confidence: 99%

“…The virulence of Mozambique and Zimbabwe isolates in hu mans is unknown. Serologic data suggest that humans do become infected with the Mozam bique strain, but thus far no human serum tested from Zimbabwe has contained antibody to any of the three arenaviruses [5],…”

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confidence: 99%

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An Arenavirus Isolated from Wild-Caught Rodents (<i>Praomys</i> Species) in the Central African Republic

et al. 1983

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We isolated nine arenavirus strains from blood and/or tissues of Praomys sp. rodents wild-caught in derived savannah and forest gallery areas of the Central African Republic (CAR). Convalescent serum from either humans or animals infected by Lassa, Mozambique, or lymphocytic choriomeningitis virus cross-reacted with the strains from the CAR. However, monoclonal antibodies to these three viruses gave a profile against the CAR strains suggesting major antigenic differences between each of them and the CAR virus. These data along with the fact that other African arenaviruses have been recovered from Mastomys natalensis and not Praomys suggest that this virus is significantly different from the others.

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“…It is carried by the African multi-mammate rat, Mastomys natalensis, widely spread in sub-Saharan Africa [3]. In contrast to its West African relative, LAS virus, MOP virus causes no lethal disease in guinea pigs or primates and can be used to vaccinate against LAS virus [4].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a reassortant virus derived from Lassa and Mopeia viruses

2006

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In this article we describe two new complete genomic sequences of Old World Arenaviruses: the Mopeia (MOP) virus and the reassortant MOP/LAS virus, clone 29, or ML29. This reassortant has the large (L) RNA from MOP virus and the small (S) RNA from Lassa (LAS) virus, Josiah strain. Recent studies showed that the ML29 virus is not pathogenic for mice, guinea pigs, or macaques, can completely protect guinea pigs from Lassa virus, and elicit vigorous cell-mediated immunity in immunized monkeys (Lukashevich, I. S., Patterson, J., Carrion, R., Moshkoff, D., Ticer, A., Zapata, J., Brasky, K., Geiger, R., Hubbard, G. B., Bryant, J., and Salvato, M. S., J Virol 79, [13934][13935][13936][13937][13938][13939][13940][13941][13942] 2005). This is a molecular characterization of a reassortant virus, which has been put forward as a live attenuated vaccine candidate against Lassa Fever. Sequence analysis of this reassortant virus revealed 5 non-conservative amino acid substitutions that distinguished it from the parental LAS and MOP viruses. Three substitutions were found outside the conserved RNA-dependent RNA polymerase (RdRp) motifs. A fourth substitution was located between the glycoprotein (GPC)-cleavage site and the putative fusion peptide of GP2. The nucleocapsid protein (NP) contained a fifth substitution in the carboxyl-terminal region of the protein. Two mutations were found within each non-coding terminus of the L segment and one mutation was located in the 3′ non-coding region of the S segment of the MOP/LAS virus. ML29 mutations in its genomic termini may have implications for the genetic stability and replication efficiency of ML29 reassortant.

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Recovery of a Lassa-Related Arenavirus in Zimbabwe

Cited by 48 publications

References 0 publications

Structural and Cell-associated Proteins of Lassa Virus

Structural and Cell-associated Proteins of Lassa Virus

An Arenavirus Isolated from Wild-Caught Rodents (<i>Praomys</i> Species) in the Central African Republic

Molecular characterization of a reassortant virus derived from Lassa and Mopeia viruses

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