Large amounts of airborne microorganisms are emitted from livestock production. These emitted microorganisms may associate with dust, and are suspected to pose a risk of airborne infection to humans in vicinity and to animals on other farms. However, the extent to which airborne transmission may play a role in the epidemic, and how dust acts as a carrier of microorganisms in the transmission processes is unknown. The authors present the current knowledge of the entire process of airborne transmission of microorganisms-from suspension and transportation until deposition and infection-and their relation to dust. The sampling and the mitigation techniques of airborne microorganisms and dust in livestock production systems are introduced as well.Airborne Microorganisms and Dust in Livestock Houses 1073 airborne microorganisms and dust from livestock production systems, as well as the factors affecting their concentrations. In section 3, the physical and biological decay of airborne microorganisms and dust during transmission is described. In section 4, the deposition of airborne microorganisms and dust in respiratory tracts, and the infective dose of pathogenic microorganisms to animals are introduced. In section 5, the strategies and techniques for sampling microorganisms and dust in livestock production systems are proposed. In section 6, the mitigation techniques are described.