1984
DOI: 10.1016/0304-4211(84)90019-1
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Recovery of cell cultures and their biosynthetic capacity after storage of Dioscorea deltoidea and Panax ginseng cells in liquid nitrogen

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Cited by 45 publications
(18 citation statements)
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“…In contrast, lower accumulation of dry cell weight was recorded for cryopreserved Taxus chinensis cell culture when compared with the untreated control in the course of 40-day cultivation, however, paclitaxel production was retained at the same level [ 246 ]. Diosgenine, sitosterol and stigmasterols content remained unchanged after cryopreservation of Dioscorea deltoidea cell cultures [ 131 ]. The same profi les of the relative DNA content have been recorded for cell cultures of Ginkgo biloba in the course of 24-month cultivation followed by cryopreservation [ 237 ].…”
Section: Cryopreservation Of Plant Cell Cultures With Enhanced Isoprementioning
confidence: 97%
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“…In contrast, lower accumulation of dry cell weight was recorded for cryopreserved Taxus chinensis cell culture when compared with the untreated control in the course of 40-day cultivation, however, paclitaxel production was retained at the same level [ 246 ]. Diosgenine, sitosterol and stigmasterols content remained unchanged after cryopreservation of Dioscorea deltoidea cell cultures [ 131 ]. The same profi les of the relative DNA content have been recorded for cell cultures of Ginkgo biloba in the course of 24-month cultivation followed by cryopreservation [ 237 ].…”
Section: Cryopreservation Of Plant Cell Cultures With Enhanced Isoprementioning
confidence: 97%
“…The fi rst suspension cell culture from P. ginseng was derived from the callus of a cambial origin in 1970 [ 131 ].…”
Section: Physiological Characteristics Of Cell Cultures Of Different mentioning
confidence: 99%
“…Most of the results about cryopreservation have been obtained from conventional techniques on suspension cells of medicinal yam, D. deltoidea being the most used ( Butenko et al, 1984;Popov and Fedorovskii, 1992;Popov and Volkova, 1994). More recent works have been done on rapid cryopreservation of callus (Chulafich et al, 1994) On the other hand, ORSTOM ability in different aspects of the long term conservation on tropical plants (Engelmann, 1991), and on encapsulation/dehydration technique applied on coffee, cassava, oil palm...etc, permitted to apply the process on apical shoot-tips of in vitro plantlets of yam (Malaurie and Trouslot 1996).…”
Section: Long-term Conservationmentioning
confidence: 99%
“…For cryoprotection, various substances are employed, individually or in binary or ternary mixtures. Sucrose (7 to 25%) is used with Panax ginseng cell suspensions (Butenko et al, 1984), DMSO (5%) with rice cells (Celia et al, 1982). A mixture of cryoprotectants is oftenly more efficient than only one component at the same total osmolarity.…”
Section: Cell Suspensionsmentioning
confidence: 99%
“…As regards post-thaw treatments, cells can eventually be washed in order to remove the cryoprotectants (Butenko et al, 1984). However, this treatment is in the majority of the cases deleterious to the survival of the cells due to the osmotic shock created.…”
Section: Cell Suspensionsmentioning
confidence: 99%