Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Qamar, Hammad

doi:10.29261/pakvetj/2019.076

Cited by 6 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carbonic anhydrase 2 (CA2) plays an important and pivotal role in various pathological pathways like bone resorption process in osteoblasts is initiated by CA2 (Borthwick et al, 2003). Higher mRNA expression of CA2 in TD chicks has been reported by Qamar et al (2019). Similar findings have been observed in our experiment regarding protein expressions of CA2.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens

Qamar¹

2020

PVJ

Self Cite

View full text Add to dashboard Cite

Among avian metabolic disorders, Tibial dyschondroplasia (TD) has a wide prevalence worldwide in chickens that are identified by non-mineralization and avascularization of the growth plate (GP). Grape seed extract (GSE) reduces the inflammation and oxidative stress by scavenging free oxygen specie and has some anti-cancer and angiogenic activity. This study was carried out to evaluate the role of GSE for the therapy of the chickens affected with TD. Arbor Acres broiler chicks (n=240) weighing 47±6g were fed starter feed till first 3 days and then portioned equally into three groups (Control, TD and GSE group; n=80 each). Thiram was incorporated in the feed of TD and GSE chicks until day 6 for inducing TD (50 mg/ kg of feed), whereas usual feed was offered to control group. All the birds in TD and GSE group showed the symptoms of TD on day 7 and GSE group was given GSE (50 mg/kg/day) intraperitoneally till the termination of the experiment. Chickens fed with thiram-feed exhibited high TD score and GP measurement. After the GSE treatment, the TD score dropped remarkably and, inflammation and TD lesions reduced. Moreover, body weight gain and liver weight were increased in comparison with TD chicks after application of GSE. GSE supplementation alleviated the adverse effects of thiram and increase in angiogenesis was observed. In conclusion, GSE recovered the TD affected broilers through downregulating the HIF-1α, Hsp90 and CA2 expressions.

show abstract

Section: Discussionsupporting

confidence: 90%

“…Similar findings have been observed in our experiment regarding protein expressions of CA2. This might be due to weakening of bones because of less deposition of minerals in TD chicks and CA2 initiated bone resorption to boost up the calcification process in cartilages (Qamar et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens

Qamar¹

2020

PVJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…This skeletal disease of chickens leads to failure in growth plate cartilage to become bone, which results in avascular cartilage and severe lameness in chickens . It is a frequently occurring leg abnormality and reported up to 30% in commercial broiler farms (Zhang et al, 2018b;Qamar et al, 2019b).…”

Section: Discussionmentioning

confidence: 99%

ASTRAGALOSIDE-IV INHIBITS TIBIAL DYSCHONDROPLASIA VIA PI3K/AKT /HIF-1α SIGNALING IN CHICKENS

Mehmood,

Karim,

Razzaq

et al. 2023

THE JAPS

View full text Add to dashboard Cite

Tibial dyschondroplasia (TD) is a common leg-pathology of meat type broiler birds which is characterized by defective tibial growth plate (GP) cartilage failing to become bone. This study deals with an attempt to treat TD using Astragaloside IV (Astr IV) from Astragalus membranaceus which have been reported to promote osteogenesis but its protective affects against TD have not been studied yet. For this purpose, we selected 180-day old chickens and distributed them in three groups alike (n= 60); control group, TD group and Astr IV group (orally @50mg/ kg body weight from 8-18 days). The chickens were slaughtered on 7, 10, 14 and 18 days. The chicken performance indicators parameters, tibial bone parameters, and expression of proteins were studied by using immunohistochemistry. The results demonstrated that in TD group production parameters including weight improvement and feed consumption were decreased compared to control group during the study period. Tibial bone parameters were lowered non-significantly (P>0.05) during the experiment except tibial GP, which was increased significantly (P<0.05) in TD chickens compared with control group on day 7, 10 and 14. While, Astr IV group indicated that performance indicators and tibia bone parameters were near to control group compared to TD group. On 14 and 18 days both TD incidence and TD score significantly (P<0.05) amplified in TD group compared to Astr IV group. The results of immunohistochemistry analysis regarding the proteins expression showed P-AKT (P<0.01), HIF-1alpha (P>0.05), and VEGF (P<0.01) expressions were increased in TD group. While the expression of AKT, PI3K and VEGFR1 were decreased non-significantly (P>0.05) in TD group chickens. However, the expression of Akt, PI3K and VEGFR1 proteins was up-regulated, while P-AKT, HIF-1alpha, and VEGF were down-regulated in Astr IV treatment group. Altogether, Astragaloside IV diminishes TD through regulating PI3K/Akt/HIF-1α signaling in chickens.

show abstract

“…Oxidative stress induces the release of Cyt-c from the mitochondria that is the key factor of apoptosis, while the antioxidant enzyme Gpx4 can eliminate lipid peroxides in the mitochondria to inhibit the production of Cyt-c and reduce apoptosis (73). DQ can also affect the caspase signal pathway by inducing oxidative stress to produce ROS and enhance the activities of caspase-3 and caspase-9 in the liver tissue (74)(75)(76)(77)(78). It is found that there is a relationship between Bax/Bcl-2 and caspase.…”

Section: Discussionmentioning

confidence: 99%

Effects of Acute Diquat Poisoning on Liver Mitochondrial Apoptosis and Autophagy in Ducks

Chen

Lin

et al. 2021

Front. Vet. Sci.

View full text Add to dashboard Cite

Diquat (DQ) is an effective herbicide and is widely used in agriculture. Due to persistent and frequent applications, it can enter into aquatic ecosystem and induce toxic effects to exposed aquatic animals. The residues of DQ via food chain accumulate in different tissues of exposed animals including humans and cause adverse toxic effects. Therefore, it is crucial and important to understand the mechanisms of toxic effects of DQ in exposed animals. We used ducks as test specimens to know the effects of acute DQ poisoning on mechanisms of apoptosis and autophagy in liver tissues. Results on comparison of various indexes of visceral organs including histopathological changes, apoptosis, autophagy-related genes, and protein expression indicated the adverse effects of DQ on the liver. The results of our experimental trial showed that DQ induces non-significant toxic effects on pro-apoptotic factors like BAX, BAK1, TNF-α, caspase series, and p53. The results revealed that anti-apoptotic gene Parkin was significantly upregulated, while an upward trend was also observed for Bcl2, suggesting that involvement of the anti-apoptotic factors in ducklings plays an important role in DQ poisoning. Results showed that DQ significantly increased the protein expression level of the autophagy factor Beclin 1 in the liver. Results on key autophagy factors like LC3A, LC3B, and p62 showed an upward trend at gene level, while the protein expression level of both LC3B and p62 reduced that might be associated with process of translation affected by the pro-apoptotic components such as apoptotic protease that inhibits the occurrence of autophagy while initiating cell apoptosis. The above results indicate that DQ can induce cell autophagy and apoptosis and the exposed organism may resist the toxic effects of DQ by increasing anti-apoptotic factors.

show abstract

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Cited by 6 publications

References 28 publications

Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens

Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens

ASTRAGALOSIDE-IV INHIBITS TIBIAL DYSCHONDROPLASIA VIA PI3K/AKT /HIF-1α SIGNALING IN CHICKENS

Effects of Acute Diquat Poisoning on Liver Mitochondrial Apoptosis and Autophagy in Ducks

Contact Info

Product

Resources

About