Recovery of human serum albumin by dual-mode chromatography from the waste stream of Cohn fraction V supernatant

Xiang, Jie; Zhang, Songping; Li, Xiunan; Zhang, Chun; Luo, Jian; Yu, Rong; Su, Zhiguo

doi:10.1016/j.chroma.2020.461451

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…The existence of albumin, which is difficult to obtain, caused various studies to be carried out to extract albumin from its source. Various methods of separating albumin from its source have been reported, including chromatography methods [13], solvent extraction [8], [14], electrophoresis [15], and adsorption [16]. Asikin et al (2018) reported that albumin could be obtained from snakehead fish 1083 (2022) 012080 IOP Publishing doi:10.1088/1755-1315/1083/1/012080 2 (Channa striata).…”

Section: Introductionmentioning

confidence: 99%

Extraction of Dissolved Protein of Channa striata from Tarakan, North Borneo as a Source of Fish Serum Albumin

Hutapea

Suprapto

Kurniawan

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Extraction of dissolved protein of Channa Striata from Tarakan, North Borneo, as a source of fish serum albumin has been done. Albumin is a water-soluble protein and it is applied in various fields, including health, industry, and technology. The many benefits of albumin make albumin very important. However, albumin is a protein found in the body of living things, so it is necessary to perform the separation process. One of the living things that contain relatively high albumin is snakehead fish (Channa striata). The dissolved protein was extracted from snakehead fish meat using water as a solvent at a temperature of 50 °C. The protein obtained was characterized using proximate analysis and Fourier Transform Infra Red (FTIR) Spectrophotometer. The proximate analysis showed that the water, the fat, and the protein content were 8.67%, 3%, and 64.18%, respectively. The FTIR spectrum showed an amide group at 1600-1700 cm−1 which is important in the albumin functional group. This study shows that snakehead fish in Tarakan, North Kalimantan has the potential as a source of fish serum albumin.

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Section: Introductionmentioning

confidence: 99%

Extraction of Dissolved Protein of Channa striata from Tarakan, North Borneo as a Source of Fish Serum Albumin

Hutapea

Suprapto

Kurniawan

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Albumin: Source, preparation, determination, applications, and prospects

Hutapea

Madurani

Syahputra

et al. 2023

Journal of Science: Advanced Materials and Devices

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Mesoporous Silica Microparticle-Protein Complexes: Effects of Protein Size and Solvent Properties on Diffusion and Loading Efficiency

Al Tahan,

Michaelides,

Somasekharan Nair

et al. 2024

Br J Biomed Sci

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Oral administration of protein-based therapeutics is highly desirable due to lower cost, enhanced patient compliance, and convenience. However, the harsh pH environment of the gastrointestinal tract poses significant challenges. Silica-based carriers have emerged as potential candidates for the delivery of protein molecules, owing to their tuneable surface area and pore volume. We explored the use of a commercial mesoporous silica carrier, SYLOID, for the delivery of octreotide and bovine serum albumin (BSA) using a solvent evaporation method in three different solvents. The loading of proteins into SYLOID was driven by diffusion, as described by the Stokes-Einstein equation. Various parameters were investigated, such as protein size, diffusion, and solubility. Additionally, 3D fluorescence confocal imaging was employed to identify fluorescence intensity and protein diffusion within the carrier. Our results indicated that the loading process was influenced by the molecular size of the protein as octreotide exhibited a higher recovery rate (71%) compared to BSA (32%). The methanol-based loading of octreotide showed uniform diffusion into the silica carrier, whereas water and ethanol loading resulted in the drug being concentrated on the surface, as shown by confocal imaging, and further confirmed by scanning electron microscopy (SEM). Pore volume assessment supported these findings, showing that octreotide loaded with methanol had a low pore volume (1.2 cc/g). On the other hand, BSA loading was affected by its solubility in the three solvents, its tendency to aggregate, and its low solubility in ethanol and methanol, which resulted in dispersed particle sizes of 223 and 231 μm, respectively. This reduced diffusion into the carrier, as confirmed by fluorescence intensity and diffusivity values. This study underscores the importance of protein size, solvent properties, and diffusion characteristics when using porous carriers for protein delivery. Understanding these factors allows for the development of more effective oral protein-based therapeutics by enhancing loading efficiency. This, in turn, will lead to advances in targeted drug delivery and improved patient outcomes.

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Recovery of human serum albumin by dual-mode chromatography from the waste stream of Cohn fraction V supernatant

Cited by 3 publications

References 31 publications

Extraction of Dissolved Protein of Channa striata from Tarakan, North Borneo as a Source of Fish Serum Albumin

Extraction of Dissolved Protein of Channa striata from Tarakan, North Borneo as a Source of Fish Serum Albumin

Albumin: Source, preparation, determination, applications, and prospects

Mesoporous Silica Microparticle-Protein Complexes: Effects of Protein Size and Solvent Properties on Diffusion and Loading Efficiency

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