Milkfish (Chanos chanos) gelatin was successfully developed as biosensor material. The milkfish bone was obtained from local restaurants in Tarakan, North Borneo, Indonesia. Gelatin was extracted from milkfish bone using acid method at 55°C. Characterization by FTIR showed that milkfish gelatin had similar functional group with commercial gelatin. The gelatin was used as biosensor material for detecting chromium. The gelatin was mixed with carbon in 1:1 ratio to form gelatin/carbon paste modified silver electrode. Electrochemical impedance spectroscopy (EIS) analysis of the gelatin/carbon paste modified silver electrode showed a better conductivity than paraffin/carbon paste modified silver electrode. Performance of the gelatin/carbon paste modified silver electrode in chromium (III) solution was conducted using cyclic voltammetry technique. Measurement was carried out at -1 V to +1 V with scan rate of 100 mV/s in acid and base condition. The best result was shown by gelatin/carbon paste modified silver electrode. It can detect chromium (III) ions at reduction potential of -0.78 V in alkaline condition. Unspecific responses were observed from silver electrode, paraffin/silver electrode, carbon/silver electrode, gelatin/silver electrode and paraffin/carbon paste modified silver electrode. This result can be concluded that the milkfish gelatin obtained have a potential to be developed as chromium (III) biosensor.

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Gelatin of milkfish (Chanos chanos) from Tarakan, North Borneo, Indonesia has been extracted successfully. The gelatin was extracted from the milkfish bone. In prior to extracting, the bone was cleaned with demineralized water and then dried at room temperature. The extraction process was done in several procedures. The first procedure has immersed the bone in 0.1 M NaOH for 48 hours. After that, it was washed until it reached neutral conditions and continued with immersing it in 0.1 M HCl for 72 hours and then washed it to get the neutral condition. The next procedure is the extraction process. It was using water solvents with a ratio of milkfish bone and water is 1: 3. The extraction process was carried out at 55ºC for 4 hours. The obtained gelatin was characterized. The results showed that moisture content 6.39%; ash content 1.92%; pH 6.1; viscosity 5.39 cP; and color 75 PtCo. This result was proved that the gelatin extraction successful.