Recovery of major royal jelly protein 1 expressed in<i>Pichia pastoris</i>in aqueous two-phase systems

Ibarra‐Herrera, Celeste C.; Torres‐Acosta, Mario A.; Mendoza‐Ochoa, Gonzalo I; Aguilar‐Yáñez, José Manuel; Rito‐Palomares, Marco

doi:10.1002/jctb.4342

Cited by 13 publications

(25 citation statements)

References 39 publications

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“…Extraction of biological products using ATPS has been successfully achieved with competitive yields and purities, proposing ATPS as a unit operation for biomolecules recovery from different sources [6][7][8][9][10]. However, the lack of practical guidelines for an effective implementation and scale-up of a continuous ATPS process has contributed to industrial reluctance towards ATPS [11].…”

Section: Introductionmentioning

confidence: 97%

Continuous enzyme aqueous two-phase extraction using a novel tubular mixer-settler in multi-step counter-current arrangement

Vázquez-Villegas

Aguilar

Rito‐Palomares

2015

Separation and Purification Technology

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Section: Introductionmentioning

confidence: 97%

Continuous enzyme aqueous two-phase extraction using a novel tubular mixer-settler in multi-step counter-current arrangement

Vázquez-Villegas

Aguilar

Rito‐Palomares

2015

Separation and Purification Technology

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“…One of the main disadvantages of using ATPS, at industrial scale, is the high quantity of materials that need to be disposed of later. Estimates show that the total operation volume can be 10–20 times larger than the input product volume used . With this in mind, research has been conducted to reduce the quantity of materials required by recycling the polymers used, especially polyethylene glycol (PEG) .…”

Section: Introductionmentioning

confidence: 99%

“…Estimates show that the total operation volume can be 10-20 times larger than the input product volume used. 15,17 With this in mind, research has been conducted to reduce the quantity of materials required by recycling the polymers used, especially polyethylene glycol (PEG). [18][19][20][21] To accomplish this the basic concept is to use back-extraction, 18 which consists of using two sequential ATPS steps, the first recovers the protein in the top phase then on the second ATPS (using the previous top phase as a base) the protein is forced to migrate to the bottom phase.…”

Section: Introductionmentioning

confidence: 99%

Economic analysis of uricase production under uncertainty: Contrast of chromatographic purification and aqueous two-phase extraction (with and without PEG recycle)

Torres‐Acosta

Aguilar‐Yáñez

Rito‐Palomares

et al. 2015

Biotechnol Progress

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Uricase is the enzyme responsible for the breakdown of uric acid, the key molecule leading to gout in humans, into allantoin, but it is absent in humans. It has been produced as a PEGylated pharmaceutical where the purification is performed through three sequential chromatographic columns. More recently an aqueous two‐phase system (ATPS) was reported that could recover Uricase with high yield and purity. Although the use of ATPS can decrease cost and time, it also generates a large amount of waste. The ability, therefore, to recycle key components of ATPS is of interest. Economic modelling is a powerful tool that allows the bioprocess engineer to compare possible outcomes and find areas where further research or optimization might be required without recourse to extensive experiments and time. This research provides an economic analysis using the commercial software BioSolve of the strategies for Uricase production: chromatographic and ATPS, and includes a third bioprocess that uses material recycling. The key parameters that affect the process the most were located via a sensitivity analysis and evaluated with a Monte Carlo analysis. Results show that ATPS is far less expensive than chromatography, but that there is an area where the cost of production of both bioprocesses overlap. Furthermore, recycling does not impact the cost of production. This study serves to provide a framework for the economic analysis of Uricase production using alternative techniques. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:126–133, 2016

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“…By contrast, TPP uses t‐butanol and ammonium sulfate to precipitate enzymes and proteins from aqueous solutions, forming three phases: a lower aqueous phase (water and ammonium sulfate), an upper t‐butanol‐rich phase, and, depending on the presence of contaminant proteins in the mixture and the concentration of ammonium sulfate used, an intermediate third phase (precipitate formed by protein or residual biomass) between the lower and upper phases . ATPS is considered to be less expensive and time‐consuming than chromatography because it saves resources by integrating and intensifying processes, but usually the amount of sample loaded into ATPS is a small fraction of the complete system . Alternatively, TPP pre‐concentrates and separates proteins in reduced volumes .…”

Section: Introductionmentioning

confidence: 99%

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

Torres‐Acosta

Morales‐Guzman

Ruiz‐Ruiz

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two‐phase systems (ATPS) and three‐phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t‐butanol hinders its acceptance. CONCLUSION Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs. © 2018 Society of Chemical Industry

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Recovery of major royal jelly protein 1 expressed inPichia pastorisin aqueous two-phase systems

Cited by 13 publications

References 39 publications

Continuous enzyme aqueous two-phase extraction using a novel tubular mixer-settler in multi-step counter-current arrangement

Continuous enzyme aqueous two-phase extraction using a novel tubular mixer-settler in multi-step counter-current arrangement

Economic analysis of uricase production under uncertainty: Contrast of chromatographic purification and aqueous two-phase extraction (with and without PEG recycle)

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

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