Recovery of substance P in the cat spinal cord after unilateral lumbosacral deafferentation

Tessler, Alan; Glazer, Ellyn J.; Artymyshyn, Roman; Murray, Marion; Goldberger, Michael E.

doi:10.1016/0006-8993(80)91294-9

Cited by 118 publications

(29 citation statements)

References 16 publications

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“…Here again, SP-ir in all areas of the brainstem remained apparently unchanged following the contralateral trigeminal rhizotomy. As was the case for CGRP-ir, the change during the second postoperative week was small; further reduction of SP-ir over that seen at 1 week postoperative or apparent recovery of it mediated by intrinsic central neurons, that reportedly occurred at a longer survival period (Tessler et al, 1980(Tessler et al, , 1981(Tessler et al, , 1984, was not noted. The following description concerns only the BSTC and the STN where asymmetry was caused by the rhizotomy.…”

Section: Sp-irmentioning

confidence: 74%

Central projection of calcitonin gene-related peptide (CGRP)- and substance P (SP)-immunoreactive trigeminal primary neurons in the rat

et al. 1997

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Substance P (SP) is implicated in transmission of primary afferent nociceptive signals. In primary neurons, SP is colocalized with calcitonin gene-related peptide (CGRP), which is another neuropeptide marker for small to medium primary neurons. CGRP coreleased with SP augments the postsynaptic effect of SP and thereby modulates the nociceptive transmission. This study demonstrates the distribution of CGRP-like immunoreactivity (-ir) and SP-ir in the lower brainstem of normal rats and after trigeminal rhizotomy or tractotomy at the level of subnucleus interpolaris (Vi). By comparing the results obtained from normal and deafferented rats, we analyzed the central projection of trigeminal primary nociceptors. The CGRP-immunoreactive (-ir) trigeminal primaries projected to the entire rostrocaudal extent of the spinal trigeminal nucleus, the principal nucleus (PrV), the paratrigeminal nucleus (paraV), and the lateral subnucleus of solitary tract nucleus (STN) on the ipsilateral side. The trigeminal primaries projecting to the spinal trigeminal nucleus, paraV and STN also contained SP-ir. The ipsilateral trigeminal primaries were the exclusive source of CGRP-ir terminals in the PrV, the Vi and the dorsomedial nucleus within the subnucleus oralis (Vo). The medullary dorsal horn (MDH) and the lateral edge of Vo received convergent CGRP-ir projection from the ipsilateral trigeminal primaries and other neurons. The glossopharyngeal and vagal primaries are candidates for the source of CGRP-ir projection to the Vo and the MDH, while the dorsal root axons supply the MDH with CGRP-ir terminals. In addition, contralateral primary neurons crossing the midline appear to contain CGRP and to terminate in the MDH.

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Section: Sp-irmentioning

confidence: 74%

Central projection of calcitonin gene-related peptide (CGRP)- and substance P (SP)-immunoreactive trigeminal primary neurons in the rat

et al. 1997

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“…A number of changes then occur over a period of days suggestive of structural reorganisation of spared afferents. A number of studies have reported that CGRP levels are replenished, along with an up-regulation of GAP-43 (LaMotte et al, 1995) and a return to normal synaptic densities in the deafferented zone (Tessler et al, 1980;Goldberger and Murray, 1982;McNeill and Hulsebosch, 1987;Wang et al, 1991;Zhang et al, 1995Zhang et al, , 1993. Studies with anatomical tracers to assess expansion of spared afferents into deafferented regions remain, however, controversial.…”

Section: Discussionmentioning

confidence: 95%

Deafferentation is insufficient to induce sprouting of A-fibre central terminals in the rat dorsal horn

et al. 1998

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The mechanism by which A-fibres sprout into lamina II of the dorsal horn of the adult rat after peripheral nerve injury, a region which normally receives input from noci- and thermoreceptive C-fibres alone, is not known. Recent findings indicating that selective C-fibre injury and subsequent degenerative changes in this region are sufficient to induce sprouting of uninjured A-fibres have raised the possibility that the structural reorganisation of A-fibre terminals is an example of collateral sprouting, in that deafferentation of C-fibre terminals alone in lamina II may be sufficient to cause A-fibre sprouting. Primary afferents of the sciatic nerve have their cell bodies located predominantly in the L4 and L5 dorsal root ganglia (DRGs), and the A-fibres of each DRG have central termination fields that show an extensive rostrocaudal overlap in lamina III in the L4 and L5 spinal segments. In this study, we have found that C-fibres from either DRG have central terminal fields that overlap much less in lamina II than A-fibres in lamina III. We have exploited this differential terminal organisation to produce deafferentation in lamina II of the L5 spinal segment, by an L5 rhizotomy, and then test whether A-fibres of the intact L4 dorsal root ganglion, which terminate within the L5 segment, sprout into the denervated lamina II in the L5 spinal segment. Neither intact nor peripherally injured A-fibres were seen to sprout into denervated lamina II after L5 rhizotomy. Sprouting was only ever seen into regions of lamina II containing the terminals of peripherally injured C-fibres. Therefore, it seems that the creation of synaptic space within lamina II is not the explanation for A-fibre sprouting after peripheral nerve section or crush, emphasising that injury-induced changes in C-fibres and subsequent chemotrophic effects in the superficial dorsal horn are the likely explanation.

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“…Following rehydration, serial 10 μm coronal sections were treated with trypsin (1.2 mg/ml PBS, Boehringer Mannheim) for 10 min, rinsed extensively and incubated for 48 h at 4 °C with a monoclonal substance P antibody (1:1000; Seralabs, Crawley, U.K.) or a serotonin antisera (1:2000). The primary serotonin antiserum and substance P antibody were localized using a modified 'ABC' technique which has been described previously [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] . Since the monoclonal substance P antibody was a rat IgG, we used biotinylated rabbit anti-rat IGg (1:1000, Vector Laboratories.…”

Section: Immunocytochemistrymentioning

confidence: 99%

“…However, we have not observed a parallel between serotonin depletion and substance P or TRH content in the spinal cord or any other brain tissue, following neonatal 5,7-DHT treatment. Tessler et al 33 have demonstrated substance P recovery in the cat, following unilateral spinal deafferentation, thus raising the possibility of reinnervation of depleted regions.…”

Section: Substance P-serotonin Co-localizationmentioning

confidence: 99%

Early postnatal administration of 5,7-dihydroxytryptamine: Effects on substance P and thyrotropin-releasing hormone neurons and terminals in rat brain

et al. 1986

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Substance P, thyrotropin-releasing hormone (TRH) and serotonin are putative neurotransmitters which have been proposed to coexist in some brain neurons Our previous immunocytochemical and biochemical studies have demonstrated that 85-100% of all serotonin neurons are destroyed following neonatal 5,7-dihydroxtryptamine (5,7-DHT) treatment In this study, we have determined the effect of neonatal 5,7-DHT and desipramine (DMI) treatment on the biochemical content and immunocytochemical localization of substance P and TRH throughout the brain Interestingly, we have observed that virtually all substance P-and TRH-immunoreactive cells in the ventral pons-medulla are destroyed by the neurotoxin. However, peptide-containing neurons in other regions were not affected Additionally, we measured the peptide content and found that TRH is significantly decreased in the spinal cord (−50%) and pons-medulla (−20%), but not in other brain regions Substance P content was not significantly altered in any region, even after a greater than 90% reduction of serotonin These data indicate that the co-localized substance P and TRH forms a small proportion of the total peptide in brain Correspondence A Towle,

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Recovery of substance P in the cat spinal cord after unilateral lumbosacral deafferentation

Cited by 118 publications

References 16 publications

Central projection of calcitonin gene-related peptide (CGRP)- and substance P (SP)-immunoreactive trigeminal primary neurons in the rat

Central projection of calcitonin gene-related peptide (CGRP)- and substance P (SP)-immunoreactive trigeminal primary neurons in the rat

Deafferentation is insufficient to induce sprouting of A-fibre central terminals in the rat dorsal horn

Early postnatal administration of 5,7-dihydroxytryptamine: Effects on substance P and thyrotropin-releasing hormone neurons and terminals in rat brain

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