Recovery of the Heart after Normothermic Ischemia Part I: Ultrastructural Findings during Postischemic Reperfusion

Mulch, J; Schaper, Jutta; Hh, Scheld; Hehrlein, F W

doi:10.1055/s-0028-1096212

Cited by 6 publications

(3 citation statements)

References 4 publications

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“…But also the depletion of energy contributes to a calcium overload of cytosol due to a reverse mode of the sarcolemmal Na/Ca exchanger. Although contraction bands may be an artifact in biopsies, they typically occur during reperfusion after myocardial ischemia [14]. In our study artifacts can be excluded by the fact, that they were not present in the in situ specimens.…”

Section: Discussioncontrasting

confidence: 46%

Comparing the ultrastructural effects of two different cardiac preparation- and perfusion-techniques in a porcine model of extracorporal long-term preservation

Aupperle

Garbade

Ullmann

et al. 2007

European Journal of Cardio-Thoracic Surgery

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Section: Discussioncontrasting

confidence: 46%

Comparing the ultrastructural effects of two different cardiac preparation- and perfusion-techniques in a porcine model of extracorporal long-term preservation

Aupperle

Garbade

Ullmann

et al. 2007

European Journal of Cardio-Thoracic Surgery

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“…During postischemic reperfusion there is a good structural recovery from ischemic damages, even following 60 minutes of anoxia. Thus, prolonged reperfusion of the empty beating heart supports morphological restitution (18).…”

Section: Discussionmentioning

confidence: 67%

Recovery of the Heart after Normothermic Ischemia: Part II: Myocardial Function during Postischemic Reperfusion

Mulch¹,

Schaper²,

Gottwik³

et al. 1979

Thorac cardiovasc Surg

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Contraction and relaxation of the canine myocardium were examined during normothermic ischemia in an isolated heart model. Decrease in the development of tension depends on the duration of ischemia. Deficient functional recovery was observed after ischemic periods extending beyond 30 minutes, in spite of reperfusion periods of over 1 hour. A decrease in compliance was observed during the anoxic period, but a persistent defect of relaxation occurred only after 60 minutes of ischemia. After this period there was also a disturbance in the autoregulative mechanisms of coronary perfusion and an uncoupling of O2-consumption and mechanical efficiency. A prolonged reperfusion period of the heart beating empty allowed ultrastructural recovery of the damaged myocardium. In contrast, functional recovery of the myocardium, as determined by several parameters of contraction and relaxation, did not correlate with ultrastructural recovery and was not improved by prolonged reperfusion.

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“…As described by other authors, functional sarcolemmal alterations may lead to an increased influx of calcium leading to hypercontraction bands (Alto et al., 1980; Schmiedl et al., 1993). Contraction bands may be an artifact following biopsy, but also typically occur during reperfusion after myocardial ischaemia (Mulch et al., 1979). In our study, artifacts could be excluded by the fact that they were not present in the in situ specimens.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Findings in Porcine Hearts After Extracorporeal Long‐term Preservation with a Modified Langendorff Perfusion System

Aupperle

Garbade

Ullmann

et al. 2007

Journal of Veterinary Medicine Series A

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Preserved ultrastructure is an important precondition for functional regeneration after heart transplantation. We investigated the effectiveness of a newly developed modified Langendorff system in extracorporeal heart perfusion. (Experiment I) Cardioplegia and cold ischaemia were performed in six pigs. Hearts were connected to a modified Langendorff system, and perfused with leucocyte depleted autologous blood. (Experiment II) The untreated hearts of three healthy pigs served as controls. Forty-seven myocardial biopsies at different timepoints (I: n = 29, II: n = 18) were investigated by transmission electronmicroscopy. Cardioplegia/hypothermia (I) induced mild-to-moderate mitochondrial swelling, mild myofibrillar degeneration in cardiomyocytes and moderate endothelial oedema. After 4 h reperfusion cardiomyocytes showed moderate myofibrillar and mild sarcolemmal damage. Moderate endothelial degeneration, mild interstitial oedema and haemorrhages appeared. Untreated hearts (II) showed severely damaged mitochondria and nuclei after 30 min while the myofibrillar structure remained unaffected until 4 h later. This is a promising model for extracorporeal heart perfusion. However, ultrastructural findings indicated that some necessary modifications to prevent cellular damages during reperfusion were needed.

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Recovery of the Heart after Normothermic Ischemia Part I: Ultrastructural Findings during Postischemic Reperfusion

Cited by 6 publications

References 4 publications

Comparing the ultrastructural effects of two different cardiac preparation- and perfusion-techniques in a porcine model of extracorporal long-term preservation

Comparing the ultrastructural effects of two different cardiac preparation- and perfusion-techniques in a porcine model of extracorporal long-term preservation

Recovery of the Heart after Normothermic Ischemia: Part II: Myocardial Function during Postischemic Reperfusion

Ultrastructural Findings in Porcine Hearts After Extracorporeal Long‐term Preservation with a Modified Langendorff Perfusion System

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