Recruitment of Factor H to the Streptococcus suis Cell Surface is Multifactorial

Roy, David; Grenier, Daniel; Segura, Mariela; Mathieu-Denoncourt, Annabelle; Gottschalk, Marcelo

doi:10.3390/pathogens5030047

Cited by 24 publications

(29 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A Fhb knockout mutant was shown to present lower survival in the presence of human blood and neutrophils and being less virulent in a pig experimental infection model . However, in our hands and using a similar mutant, we could not demonstrate any differences in phagocytosis by human macrophages and bacterial survival in whole swine blood when compared to the wild‐type strain . On the other hand, Fhbp shares similarity with the S. pneumoniae PspC protein, also possessing a factor H‐binding activity .…”

Section: How Does Streptococcus Suis Fight Against Early Host Immune contrasting

confidence: 60%

“…Another protein, a factor H‐binding protein (Fhb) , has an N ‐terminal domain that is identical to SadP and it specifically recognizes Galα1‐4Gal, the disaccharide truncation form of the glycolipid GbO3 . In addition, it has been very recently reported that host factor H is able to act as a bridge between bacteria and epithelial cells, increasing bacterial adhesion to respiratory cells . However, receptors involved in such interactions have not been yet identified.…”

Section: How Does Streptococcus Suis Establish In Mucosa and Breakdowmentioning

confidence: 99%

See 1 more Smart Citation

Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses

et al. 2016

Self Cite

View full text Add to dashboard Cite

Edited by Wilhelm JustInteractions between a bacterial pathogen and its potentially susceptible host are initiated with the colonization step. During respiratory/oral infection, the pathogens must compete with the normal microflora, resist defense mechanisms of the local mucosal immunity, and finally reach, adhere, and breach the mucosal epithelial cell barrier in order to induce invasive disease. This is the case during infection by the swine and zoonotic pathogen Streptococcus suis, which is able to counteract mucosal barriers to induce severe meningitis and sepsis in swine and in humans. The initial steps of the pathogenesis of S. suis infection has been a neglected area of research, overshadowed by studies on the systemic and central nervous phases of the disease. In this Review article, we provide for the first time, an exclusive focus on S. suis colonization and the potential mechanisms involved in S. suis establishment at the mucosa, as well as the mechanisms regulating mucosal barrier breakdown. The role of mucosal immunity is also addressed. Finally, we demystify the extensive list of putative adhesins and virulence factors reported to be involved in the initial steps of pathogenesis by S. suis.

show abstract

Section: How Does Streptococcus Suis Fight Against Early Host Immune contrasting

confidence: 60%

Section: How Does Streptococcus Suis Establish In Mucosa and Breakdowmentioning

confidence: 99%

Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…None of the latter possessed IgM cleaving activity. We hypothesized that upon IgM cleavage, Fc fragments would become detached from the bacterial surface, whereas F(ab’)2 fragments might remain attached to it as Ide Ssuis cleaves the heavy chain of IgM at the N-terminus of the C3 domain [9]. We therefore analyzed bacterial labeling with IgM using IgM-F(ab’)2 and IgM-Fc specific antibodies, that were tested in Western Blot analysis to recognize the respective parts of porcine IgM only (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Virulence factors involved specifically in complement evasion on the other hand are scarce. Proven or potential candidates are the polysaccharide capsule [8], factor H binding proteins FhB and Fhbp [9] and lastly the IgM protease Ide Ssuis [10]. Ide Ssuis , expressed universally among S. suis serotypes, is a highly specific protease, with porcine IgM as its sole substrate [11].…”

Section: Introductionmentioning

confidence: 99%

IgM cleavage by Streptococcus suis reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism

et al. 2018

View full text Add to dashboard Cite

Streptococcus suis (S. suis) causes meningitis, arthritis and endocarditis in piglets. The aim of this study was to characterize the IgM degrading enzyme of S. suis (IdeSsuis) and to investigate the role of IgM cleavage in evasion of the classical complement pathway and pathogenesis. Targeted mutagenesis of a cysteine in the putative active center of IdeSsuis abrogated IgM cleavage completely. In contrast to wt rIdeSsuis, point mutated rIdeSsuis_C195S did not reduce complement-mediated hemolysis indicating that complement inhibition by rIdeSsuis depends on the IgM proteolytic activity. A S. suis mutant expressing IdeSsuis_C195S did not reduce IgM labeling, whereas the wt and complemented mutant showed less IgM F(ab’)2 and IgM Fc antigen on the surface. IgM cleavage increased survival of S. suis in porcine blood ex vivo and mediated complement evasion as demonstrated by blood survival and C3 deposition assays including the comparative addition of rIdeSsuis and rIdeSsuis_C195S. However, experimental infection of piglets disclosed no significant differences in virulence between S. suis wt and isogenic mutants without IgM cleavage activity. This work revealed for the first time in vivo labeling of S. suis with IgM in the cerebrospinal fluid of piglets with meningitis. In conclusion, this study classifies IdeSsuis as a cysteine protease and emphasizes the role of IgM cleavage for bacterial survival in porcine blood and complement evasion though IgM cleavage is not crucial for the pathogenesis of serotype 2 meningitis.

show abstract

“…A serotype 2 non-encapsulated mutant strain showing a high percentage of hydrophobicity was used as a positive control [33]. …”

Section: Methodsmentioning

confidence: 99%

Serotype-specific role of antigen I/II in the initial steps of the pathogenesis of the infection caused by Streptococcus suis

Chuzeville

Auger

Dumesnil

et al. 2017

Vet Res

Self Cite

View full text Add to dashboard Cite

Streptococcus suis is one of the most important post-weaning porcine bacterial pathogens worldwide. The serotypes 2 and 9 are often considered the most virulent and prevalent serotypes involved in swine infections, especially in Europe. However, knowledge of the bacterial factors involved in the first steps of the pathogenesis of the infection remains scarce. In several pathogenic streptococci, expression of multimodal adhesion proteins known as antigen I/II (AgI/II) have been linked with persistence in the upper respiratory tract and the oral cavity, as well as with bacterial dissemination. Herein, we report expression of these immunostimulatory factors by S. suis serotype 2 and 9 strains and that AgI/II-encoding genes are carried by integrative and conjugative elements. Using mutagenesis and different in vitro assays, we demonstrate that the contribution of AgI/II to the virulence of the serotype 2 strain used herein appears to be modest. In contrast, data demonstrate that the serotype 9 AgI/II participates in self-aggregation, induces salivary glycoprotein 340-related aggregation, contributes to biofilm formation and increased strain resistance to low pH, as well as in bacterial adhesion to extracellular matrix proteins and epithelial cells. Moreover, the use of a porcine infection model revealed that AgI/II contributes to colonization of the upper respiratory tract of pigs. Taken together, these findings suggest that surface exposed AgI/II likely play a key role in the first steps of the pathogenesis of the S. suis serotype 9 infection.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-017-0443-4) contains supplementary material, which is available to authorized users.

show abstract

Recruitment of Factor H to the Streptococcus suis Cell Surface is Multifactorial

Cited by 24 publications

References 50 publications

Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses

Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses

IgM cleavage by Streptococcus suis reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism

Serotype-specific role of antigen I/II in the initial steps of the pathogenesis of the infection caused by Streptococcus suis

Contact Info

Product

Resources

About