Recurrent decreases of erythrocyte electrophoretic mobility in rats exposed to 1.0 R whole-body X-radiation

Abstract

Circulating erythrocytes from rats were examined up to 30 weeks post whole-body exposures of 1.0 R for alterations in the expression of net negative surface charge as measured by whole-cell microelectrophoresis in saline sorbitol. Erythrocyte electrophoretic mobility was determined in an apparatus composed of a horizontal transilluminated cylindrical chamber, equipped with a reversible, blacked platinum electrode, immersed in a water bath maintained at 25.0 +/- 1.0 degree C (Rank Brothers). In two separate experiments, recurrent decreases in the expression of net negative surface charge occurred at 10, 17, and 30 weeks post-irradiation. At these times distributional analyses of recorded erythrocyte electrophoretic mobility (EEPM) values revealed a skewing of the normally distributed EEPM population values to lower EEPM. Total sialic acid content released from hydrolyzed erythrocyte membrane preparations revealed no significant differences between erythrocytes from sham and irradiated animals. In vivo post-irradiation labeling of erythrocytes with diisopropyl-[32P] phosphorofluoridate at 4 and 33 weeks (separate experiments) indicated only a minor abbreviated erythrocyte life span at 33 weeks. Therefore, effects from low dose (1.0 R) whole-body irradiation would appear to include a recurrent defect in the expression of the net negative surface charge.

Effect of Far‐uv and Near‐uv Radiation on the Cell Surface Charge of the Protozoan

“…The cell surface charge which plays an important role in the process of cellular interaction, can be determined by measuring cellular electrophoretic mobility (Seaman and Heard, 1962). Cell electrophoresis is a technique which has been successfully used in the investigation of the effect of physical and chemical agents on the surface of various cell types (James, 1979), including the study of the effect of ionizing radiation on the cell surface of some eucaryotic cells (Sato et al, 1976;Vranska et al, 1981;Lawson et a [ . , 1982).…”