Recurrent intraoral herpes simplex virus infection

Yura, Yoshiaki; Iga, Hiroki; Terashima, Kazuyoshi; Yoshida, Hideo; Yanagawa, Tetsuo; Azuma, Masayuki; Hayashi, Yoshio; Sato, Mitsunobu

doi:10.1016/s0300-9785(86)80038-2

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…The types of clinical isolates were determined by immunofluorescent antibody staining based on reactivity with a monoclonal antibody against HSV-1 or HSV-2 (Syva Company, CA. U.S.A.) and/or by restriction endonuclease analysis of viral DNAs (20). Each virus was grown in Vero cell tnonolayers.…”

Section: Methodsmentioning

confidence: 99%

Herpes simplex virus type 1 and type 2 infection in human oral mucosa in culture

Yura

Iga

Kondo

et al. 1991

J Oral Pathology Medicine

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To examine the sensitivity of human oral mucosa to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection, human gingival mucosa explants were infected with either HSV-1 or HSV-2 in vitro and the expression of virus specific antigen was examined by the immunofluorescent antibody technique. HSV-2 antigen was found in the basement membrane, basal cell layer and lower prickle cell layer. This finding was consistent with the HSV-1 infection. Electron microscopic study revealed the presence of nucleocapsids and enveloped virus particles in the basal cells of HSV-2-infected organ cultures. These findings indicate that human gingival mucosa is sensitive to infection with HSV-2, as well as HSV-1, and that the virus may replicate in the undifferentiated epithelial cells of mucosal epithelium.

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Section: Methodsmentioning

confidence: 99%

Herpes simplex virus type 1 and type 2 infection in human oral mucosa in culture

Yura

Iga

Kondo

et al. 1991

J Oral Pathology Medicine

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“…No entanto, a quantidade de estudos que buscam analisar a suscetibilidade de outras linhagens em comparação com as comumente utilizadas é baixa (Ribeiro et al, 2018), evidenciando a importância deste estudo na tentativa de avaliar a efetividade de infecção e o comportamento dos arbovírus neurotrópicos em células IMR-32. Yura et al (1986) verificaram que culturas de células IMR-32 podem ser infectadas com vírus herpes simplex tipo 2 (HSV-2), gênero Simplexvirus, sendo observado por imunofluorescência a presença de antígenos específicos desse vírus em células infectadas.…”

Section: A B C B C a 4 Discussãounclassified

Suscetibilidade da linhagem celular de neuroblastoma humano (IMR-32) à infecção por arbovírus neurotrópicos

Gomes¹,

Almeida²,

Freitas³

et al. 2023

Braz. J. Hea. Rev.

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Introdução: Os arbovírus neurotrópicos representam um importante problema de saúde em escala global, sendo necessário a utilização de medidas de prevenção, controle e diagnóstico. Objetivo: Avaliar a efetividade da infecção de dois arbovírus neurotrópicos, vírus do Nilo Ocidental (WNV) e vírus da Encefalite de Saint Louis (SLEV), em células de neuroblastoma humano (IMR-32). Metodologia: As suspensões-estoques dos vírus foram tituladas por ensaio de placa com incubação de 120 h para WNV e 168 h para SLEV. Os isolados foram inoculados em garrafas para cultura de células que apresentavam 80% de confluência da monocamada celular e observadas por microscopia de contraste de fase por 72 h pós infecção a fim de verificar alterações morfológicas sugestivas de efeito citopático (ECP). Em seguida, as culturas foram submetidas ao ensaio de Imunofluorescência Indireta (IFI) para detecção de antígenos virais. Resultados: O título infeccioso do SLEV apresentou-se mais baixo em relação ao WNV. Em 72 h, foram observadas alterações morfológicas nas culturas inoculadas, caracterizando um ECP resultante da infecção viral, com ECP mais acentuado na cultura inoculada com WNV, confirmado pela presença de antígenos virais nas células infectadas detectados pelo teste de IFI. Conclusão: a linhagem celular IMR-32 apresentou suscetibilidade à infecção por WNV e SLEV, representando um modelo alternativo eficaz para o isolamento de arbovírus neurotrópicos.

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“…Recurrent intraoral herpes simplex lesions typically occur in adults and have a much less dramatic course (Table ). As a result, they may remain undiagnosed or mistaken for aphthous ulcerations despite the fact that aphthous ulcers do not typically affect keratinized mucosa …”

Section: Specific Infectionsmentioning

confidence: 99%

Non–plaque‐induced gingival diseases

Holmstrup

Plemons

Meyle

2018

Journal of Periodontology

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While plaque-induced gingivitis is one of the most common human inflammatory diseases, several non-plaque-induced gingival diseases are less common but often of major significance for patients. The non-plaque-induced gingival lesions are often manifestations of systemic conditions, but they may also represent pathologic changes limited to gingival tissues. A classification is proposed, based on the etiology of the lesions and includes: Genetic/Developmental disorders; Specific infections; Inflammatory and immune conditions and lesions; Reactive processes; Neoplasms; Endocrine, Nutritional and metabolic diseases; Traumatic lesions; and Gingival pigmentation.

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Recurrent intraoral herpes simplex virus infection

Cited by 7 publications

References 16 publications

Herpes simplex virus type 1 and type 2 infection in human oral mucosa in culture

Herpes simplex virus type 1 and type 2 infection in human oral mucosa in culture

Suscetibilidade da linhagem celular de neuroblastoma humano (IMR-32) à infecção por arbovírus neurotrópicos

Non–plaque‐induced gingival diseases

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