Recurrent Miscarriage and Micro-RNA Among North Indian Women

Abstract

Micro-RNAs (miRNAs) regulate diverse cellular processes such as cell differentiation, proliferation and apoptosis. Mutation in miRNAs results in various pathological conditions such as inflammation, viral infections, neurodegeneration, autoimmunity, and so on. We have evaluated the association of miR-146aC > G (rs2910164), miR-149T > C (rs2292832), miR-196a2T > C (rs11614913), and miR-499A > G (rs3746444) among patients with recurrent miscarriage (RM) and controls from North India. All the 200 patients with RM reported to experience at least 3 unexplained miscarriages before 20th week of gestation. Three hundred fertile women with no history of RMs were taken as controls. Both patients and controls were genotyped by the polymerase chain reaction amplification followed by restriction fragment length polymorphism. Variant alleles and genotypes of miR-499 A > G (Single Nucleotide Polymorphism Database [dbSNP] ID rs3746444) were found to be significant risks associated with patients having RM (odds ratio [OR] ¼ 1.98; 95% confidence interval [CI] ¼ 1.40-2.81; P value ¼ .0001) and controls (OR ¼ 3.64; 95% CI ¼ 1.33-9.94; P value ¼ .0109). A significant susceptible effect was found at allelic level in miR-196aT > C (dbSNP ID rs11614913) and miR-499 A > G (dbSNP ID rs3746444).

Association Study of Non-Coding RNA miR-499 and miR196a2 Gene Polymorphisms with the Risk of Idiopathic Recurrent Pregnancy Loss

“…Importantly, similar to the miR196a2, we did not find a positive significant association between miR499 polymorphism the increased risk of RPL compared to control group. A similarity to our findings, Parveen et al, recorded that miR-499 A > G gene polymorphism considerably significantly elevated risk in the patients with RPL compared to the controls (19). In addition, Jeon et al, was ascertained the combination of miR196a2CC and miR-499AG + GG synergistic effects to RPL (12).…”

“…Contradictory to our results, Jeon et al, reported significantly different frequencies of the miR-196a2 CC in the patients compared to control group (12). Similarity, Parveen et al, assessed the potential of the risk of miR-196a2 polymorphism between women suffering from of unexplained RPL and normal group in the North Indian women, which was disclosed as an increased risk in allelic level in miR-196a2 T > C of RPL (19). In addition, Jeon et al, adjusted this nucleotide variation in the aborted embryones, and the findings showed the significantly different frequency of the miR-196a2 CC genotypes compared with control subjects (4).…”

Maternal genetic polymorphisms and unexplained recurrent miscarriage: a systematic review and meta‐analysis

“…It is well worth mentioning that miRNA are non-coding RNAs that mediate transcription and degradation of mRNA. Although there are currently few related studies, attempting to clarify the association between miRNA mutant and RM may provide a new approach for the exploration of RM etiology (26,27). Genetic polymorphisms directly or indirectly related to coagulation or fibrinolysis processes have also been widely studied in association with RM.…”

Association between the TOX3 rs3803662 C>T polymorphism and recurrent miscarriage in a southern Chinese population

“…Recent studies have shown a link between abortion and breast cancer risk, and induced abortion may be a risk factor for breast cancer. [9][10][11] Therefore, it is helpful to understand the causes of RM by studying whether some genes involved in breast cancer pathogenesis are associated with RM. 8 In addition, other studies have shown that certain genetic polymorphisms are associated with breast cancer and may also be related to abortion, for example, the MDM2 Del1518 polymorphism and miR-196a (rs11614913).…”

“…8 In addition, other studies have shown that certain genetic polymorphisms are associated with breast cancer and may also be related to abortion, for example, the MDM2 Del1518 polymorphism and miR-196a (rs11614913). [9][10][11] Therefore, it is helpful to understand the causes of RM by studying whether some genes involved in breast cancer pathogenesis are associated with RM.…”