Approximately 382 million people worldwide have diabetes, 1 half of the prevalent cases are not known or diagnosed, half of those diagnosed are not treated, and half of those treated are not controlled. 2,3 One of the reasons for poor treatment compliance is injection anxiety causing 20% of insulin users to sometimes skip their injections, and 10% to restrict their number of injections. 4 As many as 94% of insulin users exhibit symptoms of anxiety, distress, or phobia around blood and injury from injections, 5 22% of insulin users have to mentally prepare themselves for injections, 6 and 33% of insulin users dread their injections. 7 This underlines why it is of high importance to develop needle designs that cause as little fear, injury, and pain as possible. Pain perception is one of the preferred methods to evaluate new needle design. 8-18 Studies have shown how needle diameter correlates with both the magnitude of the perceived pain, typically measured on visual analog scales (VASs), and with pain occurrence, that is, how often the needle causes pain sensation. 11-13 However, pain is a subjective measure with a large number of biasing variables causing data with high variance. Therefore, a high sample size is needed to detect differences in pain, which makes it both costly and time-consuming to carry out the clinical trials. One alternative way to obtain information about the needle impact on tissue is to use animal models, where histology can be used to assess tissue trauma from, for example, a needle insertion. The needle insertion can cause tissue bleeding and initiate inflammation in the tissue. Pig models are especially useful when examining skin disease and wound 531099D STXXX10.