Red Cell Volume Circulating and Total as Determined by Radio Iron

Hahn, P. F.; Balfour, William M.; Ross, Joseph F.; Bale, William F.; Whipple, G. H.

doi:10.1126/science.93.2404.87

Cited by 47 publications

(16 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In some cases of refractory anemia the plasma iron turnover rate is reduced; in others it is increased. 5. Iron turnover data are useful in the management and diagnosis of polycythemia vera.…”

Section: Sum Marymentioning

confidence: 99%

Plasma and Red Cell Iron Turnover in Normal Subjects and in Patients Having Various Hematopoietic Disorders 1

Huff¹,

Hennessy²,

Austin³

et al. 1950

J. Clin. Invest.

325

View full text Add to dashboard Cite

One of the obvious uses which may be made of a tracer in biological studies is the determination of rates. Zilversmitt and associates (1) and more recently London (2) have called attention to this use of a tracer. Only when the isotope can be added without effectively changing the amount or concentration of material in a steady state system can such an application be made. Not until recently has radio iron been available which had sufficiently high specific activity to tag plasma iron without changing its concentration. The amount of iron leaving and entering the plasma would be significant in determining an abnormal turnover rate in some other system of the body containing iron, for example, the red cells. The present theory of iron metabolism conceives of plasma as a pool into which iron is returned before being resynthesized into the complex organic substances, hemoglobin, myoglobin, cytochromes, peroxidases, and ferritin which are so important to body function. Since the approximate normal rate of turnover of red cell iron is known, and since the major portion of this element in the body resides in the red cells, it would be expected that abnormalities in this rate would be directly reflected in plasma iron turnover rates. This paper concerns the plasma iron and red cell iron turnover data on 75 human subjects who were given amounts of iron which did not alter the steady state systems. It is shown that such turnover rates do, indeed, agree with the clinical and laboratory data concerning normal red cell life, abnormal rates of destruction and abnormal rates of formation of red cells. It is believed that the data ascertained from the type of study described here are of value

show abstract

Section: Sum Marymentioning

confidence: 99%

Plasma and Red Cell Iron Turnover in Normal Subjects and in Patients Having Various Hematopoietic Disorders 1

Huff¹,

Hennessy²,

Austin³

et al. 1950

J. Clin. Invest.

325

View full text Add to dashboard Cite

show abstract

“…Smith, Arnold and Whipple (2) compared the value for the red cell volume obtained from the plasma volume and hematocrit reading with that obtained by the carbon monoxide and Welcker methods, and concluded that the value for the red cell volume calculated from the plasma volume and hematocrit reading was approximately 20 per cent higher than the actual red cell volume. Hahn et al (4) determined the red cell volume of dogs by a method using radio iron. They concluded that the red cell mass in circulation determined by this method averaged about 75 per cent of the value as computed by the jugular hematocrit reading from the plasma volume (dye method).…”

Section: Resultsmentioning

confidence: 99%

Demonstration That the Cell Plasma Ratio of Blood Contained in Minute Vessels Is Lower Than That of Venous Blood

Ebert¹,

Stead²

1941

J. Clin. Invest.

View full text Add to dashboard Cite

A study of the distribution of the red cells in the circulating blood is of both theoretical and practical importance. In spite of the fact that blood taken from artery, vein or finger has the same cell plasma ratio (1, 2), certain investigators (2,3,5) believe that the red cells are not uniformly distributed throughout the vascular bed but that the blood from the large vessels has a higher cell plasma ratio than the blood contained in the minute vessels. Direct observation of the minute blood vessels (6) has shown that the red cells flow in the rapidly moving central portion of the stream and that there is a slow moving clear layer of plasma adjacent to the wall of the vessel. Because of the size of this peripheral layer of clear plasma in the minute blood vessels, it has been suggested that the cell plasma ratio of blood contained in the minute vessels is lower than that of blood from the large vessels.The cell plasma ratio of the blood contained in the minute vessels has not been determined because of the difficulty in obtaining a sample of this blood. Blood collected from a vein or from the cut ends of minute vessels is representative of the blood flowing from the minute vessels but it is not necessarily representative of the blood contained within the minute vessels. The blood contained within the minute vessels consists not only of the central core of red cells and plasma which is flowing rapidly into the veins, but also of the slower moving peripheral layer of plasma.In this study a technique for obtaining a portion of the blood actually contained within the minute vessels is described. To obtain this blood it is necessary (1) to obstruct the arterial inflow to the part, (2) to empty the blood out of the large vessels, and (3) to force the blood from the minute vessels into the veins where it can be collected. The hemoglobin concentration and protein concentration of the venous blood and of the blood contained within the minute vessels were compared. METHODFifteen experiments were performed on six normal male subjects. All determinations were made on fasting subjects who had rested in the horizontal position for 30 minutes. A blood pressure cuff was placed loosely on the arm, care being taken not to cause venous distention. A needle, with the point toward the wrist, was introduced into the antecubital vein distal to the cuff. In most of the experiments the vein was entered without a tourniquet. When a tourniquet was used, blood was allowed to drip from the needle for 3 minutes before proceeding. A sample of venous blood was then taken. Immediately thereafter the arterial inflow to the arm was stopped by suddenly inflating the cuff on the arm to a pressure of 300 mm. of Hg. The forearm and hand were then elevated and the bl6od (usually 40 to 60 cc.) was removed by a syringe. When no more blood could be obtained, an Esmarch's bandage was applied to the forearm, beginning at the wrist, and the remaining blood was milked toward the needle. About 3 to 6 cc. of blood were obtained during the application of the ...

show abstract

“…per kgm. This assumption was probably no more inaccurate, as far as the red cell volume is concerned, than would have been an estimation of the blood volume by the standard dye procedures (37).…”

Section: Methodsmentioning

confidence: 99%

Absorption of Ferrous and Ferric Radioactive Iron by Human Subjects and by Dogs 1

Moore¹,

Dubach²,

Minnich³

et al. 1944

J. Clin. Invest.

121

View full text Add to dashboard Cite

Red Cell Volume Circulating and Total as Determined by Radio Iron

Cited by 47 publications

References 5 publications

Plasma and Red Cell Iron Turnover in Normal Subjects and in Patients Having Various Hematopoietic Disorders 1

Plasma and Red Cell Iron Turnover in Normal Subjects and in Patients Having Various Hematopoietic Disorders 1

Demonstration That the Cell Plasma Ratio of Blood Contained in Minute Vessels Is Lower Than That of Venous Blood

Absorption of Ferrous and Ferric Radioactive Iron by Human Subjects and by Dogs 1

Contact Info

Product

Resources

About