Red pine ( Pinus brutia Ten ) bark tree extract preserves sperm quality by reducing oxidative stress and preventing chromatin damage

Abstract

This study aimed to investigate the effectiveness of using red pine bark tree extract (P; Pinus brutia Ten) as a TRIS extender in an attempt to prevent oxidative stress in bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via an artificial vagina and pooled. They were separated into five specimens and diluted with Tris extender consisting of P (200, 100, 50 and 25 µg/ml) and P free (control; C) up to a final concentration of 16 × 106 per straw. All specimens were equilibrated for a period of 4 hr at a temperature of 4°C, following which they were filled in 0.25‐ml French straws and frozen. Addition of P resulted in favourable tail length in comparison with C (p < .05). The lowest malondialdehyde levels and the highest glutathione levels were detected in all P groups (p < .05). Supplementation with P did not show advanced results in terms of total, progressive sperm motility and total abnormality in comparison with C (p > .05). In conclusion, it has been shown that although P added to a Tris extender does not have a positive effect on sperm motility, it prevents chromatin damage by reducing oxidative stress, in addition to reducing head abnormalities when used at the amount of 50 μg/ml.

The Cryoprotective Potential of Propolis Supplemented in Frozen-Thawed Bull Semen: Biochemical and Physiological Findings

“…Caffeic acid, one of the most effective components of P, was found to reduce DNA damage in bull semen at 50 and 100 μM doses after freezing-thawing (Soleimanzadeh et al 2020). In our previous studies, we found that various plant extracts protect DNA integrity by reducing oxidative damage that may occur after freezingthawing in bull semen (Avdatek et al 2018;Taşdemir et al 2020). Our results, and other studies supporting us, have revealed the positive effect of dose-dependent antioxidant supplements on DNA damage as a result of the synergistic effect with diluents and other substances in the intracellular endogenous antioxidant system.…”

“…The application of natural antioxidant additives in sperm cryopreservation has been successfully tried by many researchers so far and positive results have also been obtained (Büyükleblebici et al 2016;Gulhan 2019; Kumar et al 2019; Taşdemir et al 2020). The use of natural products has come to the fore as an alternative to the possible toxic effects of synthetic prophylactic drugs on human and animal fertility.…”

Supplementation of cryoprotectant withPinus massonianabark extract improves human sperm vitality and fertility potential

“…Recently, it has been applied to organ transplantation preservation solution and achieved good results in mouse model 39 . Compared with recent research using 50μg/mL red pine bark tree extract ( Pinus brutia Ten ) as a TRIS extender in the cryopreservation of bull spermatozoa, 40 PMBE could significantly improve human sperm vitality and function. However, the improvement of PMBE on chromatin damage was not obvious.…”

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

“…There is an international demand for using natural medical sources in semen extenders of different animals [134,135], for example, strawberry [136], green tea [134], virgin coconut oil [30], pomegranate [135], and Pinus brutia [137,138], among others. The effects of several plant extracts on fertility have been demonstrated as antioxidants in many animal species due to their free radical scavenging properties [139].…”

“…For virgin oil addition, researchers found that Tris-based extenders containing 2% virgin oil did not improve the quality of parameters for freeze-thawed bull semen but enhanced the quality of parameters for chilled bull semen [30] An experimental study illustrated that adding P. brutia to the semen extender does not improve parameters such as motility but prevents chromatin damage and reduces oxidative stress and sperm abnormalities when used at a concentration of 50 μg/mL [137].…”