Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD

Cornebise, Clarisse; Courtaut, Flavie; Taillandier-Coindard, Marie; Valls-Fonayet, Josep; Richard, Tristan; Monchaud, David; Aires, Virginie; Delmas, Dominique

doi:10.3390/molecules25235564

Cited by 6 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect could be explained by synergic interactions between the compounds present in the mixture, as we previously observed for RSV and the flavonoid quercetin in a colorectal cancer context [29]. Mechanistically, the reduction of the secreted VEGF-A levels resulted from the action of the polyphenol mixture on the VEGF receptor (VEGF-R)/mitogen-activated protein kinases (MAPKs) molecular pathway [30]. Hence, the use of such a formulation may be of value for an AMD prevention strategy.…”

Section: Introductionmentioning

confidence: 68%

Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells

Cornebise

Perus

Hermetet

et al. 2023

Cells

Self Cite

View full text Add to dashboard Cite

Age-related macular degeneration (AMD) is one of the most commonly occurring ocular diseases worldwide. This degenerative condition affects the retina and leads to the loss of central vision. The current treatments are focused on the late stage of the disease, but recent studies have highlighted the importance and benefits of preventive treatments and how good dietary habits can reduce the risk of progression to an advanced form of the disease. In this context, we studied whether resveratrol (RSV) or a polyphenolic cocktail, red wine extract (RWE), are able to prevent the initiating events of AMD (i.e., oxidative stress and inflammation) in human ARPE-19 retinal pigment epithelial (RPE) cells and macrophages. This study highlights that RWE and RSV can prevent hydrogen peroxide (H2O2) or 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress and can subsequently prevent DNA damage via the inhibition of the ATM (ataxia telangiectasia-mutated)/Chk2 (checkpoint kinase 2) or Chk1 signaling pathways, respectively. Moreover, ELISA assays show that RWE and RSV can prevent the secretion of proinflammatory cytokines in RPE cells and in human macrophages. Interestingly, RWE exhibits a greater protective impact compared to RSV alone, even though RSV was more concentrated when used alone than in the red wine extract. Our results suggest that RWE and RSV may have potential interest as preventive nutritional supplementations against AMD.

show abstract

Section: Introductionmentioning

confidence: 68%

Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells

Cornebise

Perus

Hermetet

et al. 2023

Cells

Self Cite

View full text Add to dashboard Cite

show abstract

“…Proanthocyanidins inhibit the expression of HIV-secreting coreceptors in normal peripheral mononuclear cells [ 124 ].…”

Section: The Beneficial Effects Of Polyphenols On Health and Its Mole...mentioning

confidence: 99%

“…Proanthocyanidins and resveratrol enhance the expression of vascular endothelial growth factor (VEGF) in pigment cell culture [ 124 , 125 ] and animal model (hamsters) [ 126 ].…”

Section: The Beneficial Effects Of Polyphenols On Health and Its Mole...mentioning

confidence: 99%

The Utilization of Physiologically Active Molecular Components of Grape Seeds and Grape Marc

Hegedüs

Andreidesz

Szentpéteri

et al. 2022

IJMS

View full text Add to dashboard Cite

Nutritional interventions may highly contribute to the maintenance or restoration of human health. Grapes (Vitis vinifera) are one of the oldest known beneficial nutritional components of the human diet. Their high polyphenol content has been proven to enhance human health beyond doubt in statistics-based public health studies, especially in the prevention of cardiovascular disease and cancer. The current review concentrates on presenting and classifying polyphenol bioactive molecules (resveratrol, quercetin, catechin/epicatechin, etc.) available in high quantities in Vitis vinifera grapes or their byproducts. The molecular pathways and cellular signaling cascades involved in the effects of these polyphenol molecules are also presented in this review, which summarizes currently available in vitro and in vivo experimental literature data on their biological activities mostly in easily accessible tabular form. New molecules for different therapeutic purposes can also be synthesized based on existing polyphenol compound classes available in high quantities in grape, wine, and grape marc. Therefore an overview of these molecular structures is provided. Novel possibilities as dendrimer nanobioconjugates are reviewed, too. Currently available in vitro and in vivo experimental literature data on polyphenol biological activities are presented in easily accessible tabular form. The scope of the review details the antidiabetic, anticarcinogenic, antiviral, vasoprotective, and neuroprotective roles of grape-origin flavonoids. The novelty of the study lies in the description of the processing of agricultural by-products (grape seeds and skins) of industrial relevance, and the detailed description of the molecular mechanisms of action. In addition, the review of the clinical therapeutic applications of polyphenols is unique as no summary study has yet been done.

show abstract

“…In ARPE-19 cells subjected to oxysterols-induced stress, resveratrol addition inhibited the secretion of VEGF [ 58 ]. In another study, ARPE-19 cells were treated with red wine extract containing resveratrol, among other polyphenols, which decreased VEGF-A secretion [ 59 ].…”

Section: Age-related Macular Degenerationmentioning

confidence: 99%

Dietary Antioxidants in Age-Related Macular Degeneration and Glaucoma

2021

View full text Add to dashboard Cite

Age-related macular degeneration (AMD) and glaucoma are ophthalmic neurodegenerative diseases responsible for irreversible vision loss in the world population. Only a few therapies can be used to slow down the progression of these diseases and there are no available treatment strategies for reversing the degeneration of the neural retina. In AMD, the pathological process causes the malfunction and damage of the retinal pigmented epithelium and photoreceptors in the macula. In glaucoma, damage of the retinal ganglion cells and their axons is observed and treatment strategies are limited to intraocular pressure lowering. Therefore, other prophylactic and/or therapeutic methods are needed. Oxidative stress is involved in the neurodegenerative process accompanying both AMD and glaucoma; therefore, the use of antioxidant agents would clearly be beneficial, which is supported by the decreased prevalence and progression of AMD in patients adherent to a diet naturally rich in antioxidants. Dietary antioxidants are easily available and their use is based on the natural route of administration. Many preclinical studies both in vitro and using animal models of retinal degeneration showed the efficacy of dietary antioxidants, which was further proved in clinical trials. Resveratrol is beneficial both in AMD and glaucoma animal models, but confirmed only among AMD patients. For AMD, carotenoids and omega-3 fatty acids were also proved to be sufficient in preventing neurodegeneration. For glaucoma, coenzyme Q10 and alpha-lipoic acid showed efficacy for decreasing retinal ganglion cell loss and inhibiting the accompanying destructive processes. Interestingly, the benefits of vitamins, especially vitamin E was not confirmed, neither in preclinical nor in clinical studies.

show abstract

Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD

Cited by 6 publications

References 30 publications

Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells

Red Wine Extract Prevents Oxidative Stress and Inflammation in ARPE-19 Retinal Cells

The Utilization of Physiologically Active Molecular Components of Grape Seeds and Grape Marc

Dietary Antioxidants in Age-Related Macular Degeneration and Glaucoma

Contact Info

Product

Resources

About