2013
DOI: 10.1111/1755-0998.12138
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Redesign of PCR primers for mitochondrial cytochrome c oxidase subunit I for marine invertebrates and application in all‐taxa biotic surveys

Abstract: DNA barcoding is a powerful tool for species detection, identification and discovery. Metazoan DNA barcoding is primarily based upon a specific region of the cytochrome c oxidase subunit I gene that is PCR amplified by primers HCO2198 and LCO1490 ('Folmer primers') designed by Folmer et al. (Molecular Marine Biology and Biotechnology, 3, 1994, 294). Analysis of sequences published since 1994 has revealed mismatches in the Folmer primers to many metazoans. These sequences also show that an extremely high level … Show more

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Cited by 833 publications
(606 citation statements)
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“…For barcoding, tissue was subsampled from each specimen and placed individually in 96-well Costar plates (Corning) for phenol DNA extraction. DNA amplification and Sanger sequencing used standard protocols (SI Text, section V.A) and previously published primers (19,20). For metabarcoding, DNA was extracted from 10 g of homogenized sessile tissue and the crushed half of the 2-mm to 500-μm and 500-to 106-μm samples using the MO-BIO Powermax Soil DNA Isolation Kit (SI Text, section V.B).…”
Section: Methodsmentioning
confidence: 99%
“…For barcoding, tissue was subsampled from each specimen and placed individually in 96-well Costar plates (Corning) for phenol DNA extraction. DNA amplification and Sanger sequencing used standard protocols (SI Text, section V.A) and previously published primers (19,20). For metabarcoding, DNA was extracted from 10 g of homogenized sessile tissue and the crushed half of the 2-mm to 500-μm and 500-to 106-μm samples using the MO-BIO Powermax Soil DNA Isolation Kit (SI Text, section V.B).…”
Section: Methodsmentioning
confidence: 99%
“…DNA processing DNA was extracted from 10 g of each homogenized sample using PowerMax Soil DNA Isolation Kit (www.mobio.com). Two genes were amplified: a 100-110 bp fragment in the v7 region of the 18S rRNA gene, using the 18S_allshorts primers (Guardiola et al, 2015; forward: 5'-TTTGTCTGSTTAATTSCG-3' and reverse: 5'-TCACAGACCTGTTATTGC-3), and a fragment of the COI gene, amplified with a modification of the forward miCOIintF primer (Leray et al, 2013): 5'-GGWACWRGWTGRACWITITAYCCYCC-3' and the reverse jgHCO2198 primer (Geller et al, 2013): 5'-TAIACYTCIGGRTGICCRAARAAYCA-3'. The forward primer incorporated two more wobble bases and two inosine nucleotides in the most degenerate positions, relative to the original miCOIintF.…”
Section: Samplingmentioning
confidence: 99%
“…For most species and OTUs, we used the primers dgLCO1490/dgHCO2198 (Meyer 2003), which are slight modifications of the universal primers LCO1490/ HCO2198 (Folmer et al 1994). For one species, Polymastia corticata Rildey & Dendy, 1886, CO1 sequences of satisfactory quality could be obtained only with the primers jgLCO1490/jgHCO2198 (Geller et al 2013).…”
Section: Taxonomic Scopementioning
confidence: 99%