Redox balance in the body: An approach to quantitation

Shapiro, Howard M.

doi:10.1016/0022-4804(72)90057-1

Cited by 63 publications

(34 citation statements)

References 70 publications

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“…A plurality of analytical methods has been devised out during last decades for assaying total antioxidant capacity in biological samples [34] [35]. Results obtained from these various methods are hardly comparable because of the different mechanisms, redox potentials, pH and differing nature of specific antioxidant detected and other analytical parameters of various assays [36].…”

Section: Methods Overviewmentioning

confidence: 99%

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A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

Ciuti¹,

Liguri²

2017

JBiSE

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A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated. A surfactant present in the reagent causes the lysis of cells in the sample. Glutathione, ascorbic acid, protein thiols and other main antioxidant substances in the sample react with a specific redox chromogen (2,6-dichlorophenolindophenol) and produce a change in the color of the reagent, which is determined photometrically at 630 nm. This new method quantifies the overall capacity of the sample to reduce a redox chromogen and it requires the minimum volumes and time. The assay can be used on a wide variety of biological samples including whole blood, serum, plasma, tissue extracts and homogenates, food, wine, fruit juice and other beverages. Results are expressed in terms of mmol/L glutathione equivalents. TAC can be assayed manually, on automatic analyzers or micro-plate readers.

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Section: Methods Overviewmentioning

confidence: 99%

“…These compounds, provided with reducing power, are either produced in the body or derived from the diet [10] [11]. It should also be underlined that individual antioxidants don't function as isolated entities, but as part of complex systems with relevant interdependence and synergistic effects.…”

Section: Introductionmentioning

confidence: 99%

A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

Ciuti¹,

Liguri²

2017

JBiSE

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“…This insulinopenic "diabeteslike state" with glucose intolerance has also been described in a patient with the Fanconi syndrome associated with hepatic glycogenosis and abnormal galactose metabolism (8). An increased L:P ratio can occur in uncontrolled diabetes (38). but the glucosuria was not a simple diabetic overflow phenomenon.…”

Section: Abnormalities Of Carbohydrate Metabolismmentioning

confidence: 81%

“…An alternate explanation for the increased L:P ratio which could also explain the diverse metabolic abnormalities is that of an altered cytoplasmic redox state or increased NADH:NAD' ratio (16,38). This would result in the shifting of certain NADH:…”

Section: (7)mentioning

confidence: 99%

Abnormalities of Carbohydrate Metabolism in Idiopathic Fanconi Syndrome

et al. 1980

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SummaryVarious metabolic studies were performed in a patient with the idio~athic Fanconi svndrome in whom constant ketonuria suegesied that organic acidemia might contribute to the metabolic acidosis. Glucose intolerance with a diminished insulin release was found after P O or IV glucose loads and after glucagon administration. An insulinopenic "diabetes-like" state has not previously been described in such patients. The patient had impaired galactose-glucose interconversion, elevated blood lactate levels, reduced pyruvate levels, and an increased 1actate:pyruvate ratio. Hepatomegaly and hypoglycemia were not present, and liver and muscle biopsies revealed no enzymatic evidence of glycogenosis. The erythrocyte UDP galactose transferase activity was normal.The patient failed to convert fructose to glucose and had a rise in blood lactate after ethanol administration. Further studies revealed no production of glucose after alanine or glycerol administration, each test being associated with elevated blood lactate levels and, after alanine, an increased 1actate:pyruvate ratio. The 1actate:pyruvate ratio was elevated after glucagon administration with increased lactate and reduced pyruvate concentrations. SpeculationThe increased 1actate:pyruvate ratio, abnormal galactose-glucose, and pyruvate-lactate interconversions suggest an altered intracellular redox state with an abnormally high NADH:NAD+ ratio.

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“…Healthy human tissue in vivo in the presence of dissolved oxygen has a redox potential (EJ of -40 mV at pH 7.2 (Shapiro, 1972). It may be assumed that as treponemal infection progresses to cause tissue necrosis, the E h value will diminish with the development of optimal conditions for organism survival.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Reducing and Other Agents on the Motility of Treponema pallidum in an Acellular Culture Medium

Chalmers

Taylor‐Robinson

1979

Journal of General Microbiology

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443The maintenance of Treponema pallidum motility was investigated in an acellular medium based on T. pallidurn immobilization test medium. The acellular medium contained cysteine, glutathione, thioglycollate and dithiothreitol as reducing agents and had a redox potential of -275 & 25 mV at pH 7.3. In an atmosphere containing 3 % 02, motile treponemes survived four times longer when calf serum and bovine serum albumin were added to the medium. The selective omission of glutathione and, particularly, thioglycollate prolonged the survival of motile treponemes almost fivefold. In addition, stored medium, in which thioglycollate had become inactive, sustained motile treponemes for longer than did freshly prepared medium. Thus, thioglycollate is toxic for the organisms. It may be omitted from the medium because low redox potentials can be achieved without it.

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Redox balance in the body: An approach to quantitation

Cited by 63 publications

References 70 publications

A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

Abnormalities of Carbohydrate Metabolism in Idiopathic Fanconi Syndrome

The Effect of Reducing and Other Agents on the Motility of Treponema pallidum in an Acellular Culture Medium

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