Redox-mediated Mechanisms Regulate DNA Binding Activity of the G-group of Basic Region Leucine Zipper (bZIP) Transcription Factors in Arabidopsis

Shaikhali, Jehad; Norén, Louise; Barajas-López, Juan de Dios; Srivastava, Vaibhav; König, Janine; Sauer, Uwe H.; Wingsle, Gunnar; Dietz, Karl‐Josef; Strand, Åsa

doi:10.1074/jbc.m112.361394

Cited by 93 publications

(87 citation statements)

References 54 publications

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“…assays (EMSAs) revealed that purified bZIP16 recombinant protein could bind tandem repeats (43) of the G-box probe and form several mobility retarded protein-DNA complexes ( Figure 1B). This result confirms previous reports that the bZIP16-G-box complex could be formed by incubating the G-box probe with a crude protein mixture containing in vitro-translated bZIP16 protein or recombinant bZIP16 proteins Shaikhali et al, 2012). The formation of bZIP16-G-box complexes was reduced in the presence of excess unlabeled G-box competitors ( Figure 1B), which suggests that bZIP16 specifically binds to the G-box cis-element.…”

Section: Results Bzip16 Is a G-box Binding Proteinsupporting

confidence: 91%

“…Therefore, bZIP16 might be a nuclear-localized transcriptional regulator. The nuclear localization of bZIP16 was previously observed by transient overexpression of bZIP16-fluorescent protein fusion proteins in either onion epidermal cells or Arabidopsis mesophyll protoplasts Shaikhali et al, 2012). To verify whether a biologically functional form of bZIP16 is indeed a nuclear protein, we introduced bZIP16-GFP (for green fluorescent protein) into the bzip16-1 mutant.…”

Section: Bzip16-green Fluorescent Protein Is a Nuclear Proteinmentioning

confidence: 95%

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Arabidopsis bZIP16 Transcription Factor Integrates Light and Hormone Signaling Pathways to Regulate Early Seedling Development

Hsieh

2012

Plant Cell

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Section: Results Bzip16 Is a G-box Binding Proteinsupporting

confidence: 91%

Section: Bzip16-green Fluorescent Protein Is a Nuclear Proteinmentioning

confidence: 95%

Arabidopsis bZIP16 Transcription Factor Integrates Light and Hormone Signaling Pathways to Regulate Early Seedling Development

Hsieh

2012

Plant Cell

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“…Fitting the observed curve of DNA-binding activity versus buffer redox potential to the Nernst equation for a two-electron reaction, a midpoint potential of 2238.4 6 1.4 mV at pH 7.5 was obtained. This potential is close to that of the GSH/ GSSG pair under these conditions (2269.6 mV; Veine et al, 1998;Bick et al, 2001) and fit in the range of 2170 to 2330 mV reported for several redox-regulated plant proteins such Rap2.4a, AtbZip16, ABI2, TRXs, and GRXs (Meinhard et al, 2002;Rouhier et al, 2008;Shaikhali et al, 2008Shaikhali et al, , 2012. It can be suggested that redox interconversions may operate under in vivo conditions to modulate the DNA-binding activity of TCP15.…”

Section: Cellular Redox Agents Modulate the Dna-binding Activity Of Tsupporting

confidence: 78%

Redox Modulation of Plant Developmental Regulators from the Class I TCP Transcription Factor Family

2013

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TEOSINTE BRANCHED1-CYCLOIDEA-PROLIFERATING CELL FACTOR1 (TCP) transcription factors participate in plant developmental processes associated with cell proliferation and growth. Most members of class I, one of the two classes that compose the family, have a conserved cysteine at position 20 (Cys-20) of the TCP DNA-binding and dimerization domain. We show that Arabidopsis (Arabidopsis thaliana) class I proteins with Cys-20 are sensitive to redox conditions, since their DNAbinding activity is inhibited after incubation with the oxidants diamide, oxidized glutathione, or hydrogen peroxide or with nitric oxide-producing agents. Inhibition can be reversed by treatment with the reductants dithiothreitol or reduced glutathione or by incubation with the thioredoxin/thioredoxin reductase system. Mutation of Cys-20 in the class I protein TCP15 abolished its redox sensitivity. Under oxidizing conditions, covalently linked dimers were formed, suggesting that inactivation is associated with the formation of intermolecular disulfide bonds. Inhibition of class I TCP protein activity was also observed in vivo, in yeast (Saccharomyces cerevisiae) cells expressing TCP proteins and in plants after treatment with redox agents. This inhibition was correlated with modifications in the expression of the downstream CUC1 gene in plants. Modeling studies indicated that Cys-20 is located at the dimer interface near the DNA-binding surface. This places this residue in the correct orientation for intermolecular disulfide bond formation and explains the sensitivity of DNA binding to the oxidation of Cys-20. The redox properties of Cys-20 and the observed effects of cellular redox agents both in vitro and in vivo suggest that class I TCP protein action is under redox control in plants.TCP transcription factors constitute a family of plant developmental regulators (Martín-Trillo and Cubas, 2010;Uberti Manassero et al., 2013). The name of the family stands for the three first characterized members: TEOSINTE BRANCHED1, CYCLOIDEA, and PRO-LIFERATING CELL FACTOR1 (Cubas et al., 1999). These factors bind DNA through the TCP domain, a conserved region of approximately 60 amino acids that also participates in protein dimerization. The TCP domain contains a basic N-terminal region involved in DNA recognition, followed by a helix-loop-helix (HLH) motif similar to the one present in basic helix-loop-helix (bHLH) transcription factors (Aggarwal et al., 2010;Martín-Trillo and Cubas, 2010). Target sites recognized by TCP proteins are different from those bound by bHLH proteins (Kosugi and Ohashi, 2002;Viola et al., 2012). This is probably due to the fact that the respective basic regions differ markedly in composition and structure. Based on sequence homology, two main classes of TCP domains can be described. Class II proteins affect leaf and petal development and also influence shoot branching, among other processes

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“…The transcription factors regulated by ROS result in the transcription of a large number of genes (Maxwell et al, 2002;Miller et al, 2010;Smykowski et al, 2010;Shaikhali et al, 2012;Munné-Bosch et al, 2013). Recently, there has been a growing interest in understanding the regulation of senescence at the level of systems biology, using bioinformatics tools to build models for transcription factor networks (Penfold and Buchanan-Wollaston, 2014).…”

Section: Ros Generation and Scavenging: Spatiotemporal Effectsmentioning

confidence: 99%

Production and Scavenging of Reactive Oxygen Species and Redox Signaling during Leaf and Flower Senescence: Similar But Different

2016

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ORCID IDs: 0000-0003-3830-5857 (H.R.); 0000-0001-6523-6848 (S.M.-B.).Reactive oxygen species (ROS) play a key role in the regulation of many developmental processes, including senescence, and in plant responses to biotic and abiotic stresses. Several mechanisms of ROS generation and scavenging are similar, but others differ between senescing leaves and petals, despite these organs sharing a common evolutionary origin. Photosynthesis-derived ROS, nutrient remobilization, and reversibility of senescence are necessarily distinct features of the progression of senescence in the two organs. Furthermore, recent studies have revealed specific redox signaling processes that act in concert with phytohormones and transcription factors to regulate senescence-associated genes in leaves and petals. Here, we review some of the recent advances in our understanding of the mechanisms underpinning the production and elimination of ROS in these two organs. We focus on unveiling common and differential aspects of redox signaling in leaf and petal senescence, with the aim of linking physiological, biochemical, and molecular processes. We conclude that the spatiotemporal impact of ROS in senescing tissues differs between leaves and flowers, mainly due to the specific functionalities of these organs.

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Redox-mediated Mechanisms Regulate DNA Binding Activity of the G-group of Basic Region Leucine Zipper (bZIP) Transcription Factors in Arabidopsis

Cited by 93 publications

References 54 publications

Arabidopsis bZIP16 Transcription Factor Integrates Light and Hormone Signaling Pathways to Regulate Early Seedling Development

Arabidopsis bZIP16 Transcription Factor Integrates Light and Hormone Signaling Pathways to Regulate Early Seedling Development

Redox Modulation of Plant Developmental Regulators from the Class I TCP Transcription Factor Family

Production and Scavenging of Reactive Oxygen Species and Redox Signaling during Leaf and Flower Senescence: Similar But Different

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