Redox Processes in the Plasma Membrane

Crane, F.L.

doi:10.1007/978-3-642-74522-5_5

Cited by 20 publications

(22 citation statements)

References 102 publications

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“…Except of slight hyperosmtic shock, abrupt hyperosmotic shock and hypoosmotic shock greatly decreased the rates of NADH oxidation and Fe(CN) 6 3-reduction (Tab 1), which is in accordance with the phenomenon observed by Crane and Barr under salt stress [3] and Qiu et al under water stress [4]. The electron transport chains of plasmalemma redox system are multiple [11,12 ]: innerside or outerside cis-plasmalemma electron transfer; inward or outward transplasmalemma electron transfer etc. The enzymes responsible for electron transfer are NAD(P)H: Fe(CN) 6 3-standard system oxidoreductase, NAD(P)H: Fe 3+ chelator oxidoreductase, NAD(P)H: O 2 oxidoreductase, peroxidase, nitrate reductase, and so on [2,12 ].…”

Section: Discussionsupporting

confidence: 84%

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Effect of osmotic shock on the redox system in plasma membrane of Dunaliella salina

1996

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The unicellular halotolerant alga Dunaliella salina had the ability to oxidize NADH and reduce Fe(CN) 6 3-. The redox reactions were to some extent stimulated by slight hyperosmotic shock (2.0 mol/L → 2.6 mol/L NaC1), but markably inhibited by abrupt hyperosmotic shock (2.0 mol/L → 3.5 mol/L NaC1) and hypoosmotic shock (2.0 mol/L → 1.0 mol/L NaC1; 2.0 mol/L→0.67 mol/L NaC1).With the adaptation of algal cells to osmotic shock by accumulating or degrading intracellular glycerol, the plasmalemma redox activities were also restored.

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Section: Discussionsupporting

confidence: 84%

“…Accompanying trans-plasmalemma electron transport, H + may extrude through a protonated electron carrier (ubiquinone), or H + channels, or the conformational change of redox proteins [2,11]. Under osmotic shock conditions, H + efflux was inhibited by vanadate and DES, but almost unaffected by 8-hydroxyquinoline (Tab 4).…”

Section: Discussionmentioning

confidence: 99%

Effect of osmotic shock on the redox system in plasma membrane of Dunaliella salina

1996

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“…One such transplasma membrane electron transport system is thought to be induced in roots of nongraminaceous plants during iron deficiency and to be involved in the reduction of Fe3" to Fe2" for uptake (reviewed in ref. 17). Other, constitutive systems are thought to be involved in other processes, such as hor- ' Supported by a grant from the Swedish Natural Science Research Council.…”

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confidence: 99%

Transmembrane Electron Transport in Plasma Membrane Vesicles Loaded with an NADH-Generating System or Ascorbate

Askerlund

Larsson²

1991

Plant Physiol.

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Sugar beet (Beta vulgaris L.) leaf plasma membrane vesicles were loaded with an NADH-generating system (or with ascorbate) and were tested spectrophotometrically for their ability to reduce external, membrane-impermeable electron acceptors. Either alcohol dehydrogenase plus NAD* or 100 millimolar ascorbate was included in the homogenization medium, and right-side-out (apoplastic side-out) plasma membrane vesicles were subsequently prepared using two-phase partitioning. Addition of ethanol to plasma membrane vesicles loaded with the NADH-generating system led to a production of NADH inside the vesicles which could be recorded at 340 nanometers. This system was able to reduce 2,6-dichlorophenolindophenol-3'-sulfonate (DCIP-sulfonate), a strongly hydrophilic electron acceptor. The reduction of DCIP-sulfonate was stimulated severalfold by the K+ ionophore valinomycin, included to abolish membrane potential (outside negative) generated by electrogenic transmembrane electron flow. Fe3+-chelates, such as ferricyanide and ferric citrate, as well as cytochrome c, were not reduced by vesicles loaded with the NADH-generating system. In contrast, right-side-out plasma membrane vesicles loaded with ascorbate supported the reduction of both ferric citrate and DCIP-sulfonate, suggesting that ascorbate also may serve as electron donor for transplasma membrane electron transport. Differences in substrate specificity and inhibitor sensitivity indicate that the electrons from ascorbate and NADH were channelled to external acceptors via different electron transport chains. Transplasma membrane electron transport constituted only about 10% of total plasma membrane electron transport activity, but should still be sufficient to be of physiological significance in, e.g. reduction of Fea3 to Fe2+ for uptake.It has been suggested that the plant plasma membrane contains redox systems that transfer electrons from cytoplasmic donors to acceptors in the apoplast. One such transplasma membrane electron transport system is thought to be induced in roots of nongraminaceous plants during iron deficiency and to be involved in the reduction of Fe3" to Fe2" . 11 and 17). The nature of the electron donor(s) for the transplasma membrane electron transport system(s) is not known. Substantial evidence indicates, however, that NAD(P)H is such an electron donor (9, 11, 17).We (1) previously found that NADH-ferricyanide3 and NADH-Cyt c reductase activities were about 30% latent (i.e. could be measured only in the presence of a detergent, such as Triton X-100) with inside-out vesicles and about 80% latent with right-side-out vesicles. From these results, we concluded that both donor and acceptor sites for these activities were present on the cytoplasmic surface of the plasma membrane and that transplasma membrane electron transport from NADH to ferricyanide or Cyt c could at most constitute 30% of the total activities (1). Based on trypsin inhibition of NADH-Cyt c reductase activity (2, 21), H+-pumping capacity (21), and H+-ATPase latency using th...

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“…Møller & Crane 1990;Döring & Lüthje 1996;Lüthje et al 1997). Mammalian PM contain ubiquinones as well as other types of quinones (Møller & Crane 1990;Sun et al 1992;Villalba, Crane & Navas 1998).…”

Section: Quinonesmentioning

confidence: 99%

“…The correlation between the presence of LIAC and the plant PM was so high that LIAC was suggested to be one of the positive marker activities for the plant PM (Widell, Lundborg & Larsson 1982). By the end of the 1980s the presence of redox (or electron-transporting) constituents in the plant plasma membrane became well established and widely accepted (Møller & Lin 1986;Møller et al 1988;Møller & Crane 1990).…”

Section: Introductionmentioning

confidence: 99%