Redox properties of isradipine and its electrochemical detection in the HPLC determination of the compound in human serum

Abstract

The electrochemical behaviour of isradipine in a mixed solution of Britton-Robinson buffer (pH 11.8):acetonitrile:methanol (6:3:1, v/v) was studied by cyclic voltammetry and spectroelectrochemistry using an optically transparent thin layer electrode of carbon cloth. The cyclic voltammogram showed several peaks whose shape and potentials depended on the pH. The peak at 330 nm, corresponding to the absorbance of the dihydropyridine ring, disappeared after electrolysis at a potential that was more positive than the oxidation peak. The oxidation peak corresponds to the oxidation of the dihydropyridine ring. Peak height at pH 11.8 was proportional to isradipine concentration. On the basis of the redox properties of isradipine, HPLC was conducted applying electrochemical detection on a polybutadiene coated alumina column using an alkaline mobile phase. The method was applied for the determination of isradipine content in human serum. A good linear relationship between isradipine concentration and peak height was found in the concentration range of 2-200 ng/mL with a correlation coefficient of 0.9924. The detection limit was 0.5 ng/mL. The within-day and day-to-day variation were examined for control human serum and percentage relative standard deviation ranged from 0.5 to 6.7. Interference from many other coadministered drugs was studied in the specified experimental conditions. Photo and heat stabilities of the compound were also studied.

Newfangled Quantitative Pharmacia (Isradipine) Stratagem: Quality by Design (Qbd) Taguchi Array via Response Surface (L25 Via CCD20) Methodology

“…The preferred drug (Isradipine standard) reference procured was as kindly gifted by Pharmaceuticals Ltd (India) and lambda maximum (λ max ; Figure 1) [24,39] was measured by double beam (Jasco, UV-630) spectrophotometer method [38] using one centi-meter quartz cells. All analytical grade solvents were and reagents were purchased from Lakshya Enterprises and Mercury Lab., New-Delhi, India.…”

“…Biologically, the selected model drug pine mechanism is bound L-type of Calcium Channel blocker (CCB s ) flux of cardiac and also smooth muscles with prominent specificity via affinity and inhibiting [3][4][5][6][7][8]. Literature, for CCB s plentiful techno and estimation methods were like Titrimetric [9,10], spectroscopic [11][12][13][14][15][16][17][18], Fluorimetric [19][20][21][22][23][24][25][26], electrochemical [27][28][29], Chromatographic methods [30][31][32][33][34][35][36][37][38] already reported [39][40][41][42]. Therefore, except a few anti-BP drugs till date there is no analytical quality design (QbD) stratagem.…”

A Brief Review on Analytical Methods for Estimation of Isradipine in Pharmaceutical Formulation and Biological Matrices

“…The ISRA is the drug first choice for CAD [9].The ISRA is mentioned in British pharmacopeia (BP 2005), in which analytical method described for the analysis of ISRA. The ISRA is also mentioned in the united states pharmacopeia (USP 27,2004), in which analytical method described for the analysis of ISRA. The main objective of the present work is to describe the analytical methods for estimation of ISRA in various formulations and matrices.…”

“…The intra and inter-day studies were examined for control human serum and proportion relative standard deviation range from 0.5 to 6.7 [27].…”

Quantification of isradipine in human plasma using LC–MS/MS for pharmacokinetic and bioequivalence study

“…However these methods, possess disadvantages such as low sensitivity [6,19,26], long analytical run times [6,26], or pre-column derivatization processes [25]. The quantified data from the HPLC techniques coupled with UV detection (HPLC/UV) [21,27,28], and electrochemical detection (HPLC/ECD) [29] exhibit a LLOQ of almost 0.5 ng/ml. In addition, a spectrofluorometric method was recently published, but this method offered low sensitivity (16 ng/ml LLOQ) and needed derivatization to enhance the fluorescent intensity because isradipine itself yields little native fluorescence [30].…”