Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

Khoa, Hoang Dang; Yawson, Emmanuel; Tolani, Sagar; Wanekaya, Adam K.

doi:10.2116/analsci.24.1105

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…The inset shows the peak currents plotted vs amount of ascorbic acid added. Compared to other results we recently reported [28], the sensitivity to ascorbic acid was improved 10-fold. The catalytic oxidation peak current was linearly dependent on the ascorbic acid concentration, as shown by the linear calibration curve in the inset.…”

Section: Resultscontrasting

confidence: 78%

“…A linear relationship was observed for concentration of ascorbic acid beyond the 0.0-100 M range used (R = 0.9996, n = 10). The sensitivity was 48 A/mM, an improvement of about 10-fold from our earlier published results [28] and about a 10-fold improvement over other results reported in the literature for indirect measurements [29,30]. Fig.…”

Section: Resultssupporting

confidence: 52%

“…There was a linear relationship between the concentration of ascorbic acid added and the step size obtained (R = 0.999, n = 10). The sensitivity measured (slope of the calibration curve) was 0.003 A/M for this sensor, an improvement of nearly 23% over what we reported recently with a different system sensor [28].…”

Section: Resultsmentioning

confidence: 43%

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Redox polymer covalently modified multiwalled carbon nanotube based sensors for sensitive acetaminophen and ascorbic acid detection

Havens

Trihn

Kim

et al. 2010

Electrochimica Acta

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Section: Resultscontrasting

confidence: 78%

Section: Resultssupporting

confidence: 52%

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Redox polymer covalently modified multiwalled carbon nanotube based sensors for sensitive acetaminophen and ascorbic acid detection

Havens

Trihn

Kim

et al. 2010

Electrochimica Acta

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“…Finally, the performance offered by this flow injection approach for the simultaneous and selective detection of both AA and H 2 O 2 was evaluated by analyzing some different orange-taste soft drinks purchased from local supermarkets, which were spiked with controlled amounts of both AA and H 2 O 2 . In fact, we have preferred to estimate the accuracy of the method here proposed by the recovery approach, instead of comparing our findings with those found with other procedures proposed previously which suffer from several interferences [19] and are in particular unable to assure reliable results for the selective determination of these two analytes displaying antagonist redox properties.…”

mentioning

confidence: 91%

“…They are mostly based on titrimetric [5], chromatographic [6,7], spectrometric [6,[8][9][10], enzymatic [11][12][13] and electroanalytical [6,12,[14][15][16][17][18] approaches. Almost all of these methods suffer from interference from several species [19], among which hydrogen peroxide (H 2 O 2 ) is by far the worst. The concomitant presence of H 2 O 2 with AA is frequently encountered because it plays a significant role in pharmaceutical, cosmetic and food industry as an oxidizing, bleaching and sterilizing agent, it being widely used as a cleaning-in-place oxidizer.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Ascorbic Acid and Hydrogen Peroxide by Flow‐Injection Analysis with a Thin Layer Dual‐Electrode Detector

et al. 2010

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Based on preliminary voltammetric investigations at unmodified and modified electrodes in aqueous solutions buffered at different pHs, a profitable thin layer dual-electrode detector was developed for the selective and simultaneous determination of ascorbic acid (AA) and hydrogen peroxide present in the same samples. It consists of a small thickness (0.1 mm) cell, used as a detector for a flow injection system, in which a glassy carbon (GC) and a polyeugenol coated Pt electrode are encased. The GC electrode is selective for AA, thanks to the potential applied (0.3 V vs. Ag/AgCl,Cl À sat ), while H 2 O 2 alone can be oxidized at the Pt electrode (0.75 V), thanks to the size selectivity and electrostatic screen properties displayed by the polyeugenol coating layer which prevents oxidation of ascorbate anions. Repeatable sharp peaks (AE 4.5 %) were detected for both analytes over wide linear ranges (0.005-1.000 mM) and detection limits of about 10 À6 M (176 ppb) and 5 10 À7 M (16 ppb) were inferred for AA and H 2 O 2 respectively. This flow injection approach was applied to the analysis of some orange-taste soft drinks, used as prototype real samples, spiked with controlled amounts of both analytes, thus inferring good recoveries which pointed out that deviations never exceeding 5 % affected the relevant accuracy.

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Simultaneous Determination of Peroxide Hydrogen and Ascorbic Acid by Capillary Electrophoresis with Platinum Nanoparticles Modified Micro‐disk Electrode

et al. 2017

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In this study, the first application of a capillary zone electrophoresis‐electrochemical detection (CE‐ECD) method for concurrent determination of hydrogen peroxide (H2O2) and ascorbic acid (AA), was developed using the Pt nanoparticles (PtNPs) modified Pt micro‐disk electrode (PtME). The electrocatalytic activity of the modified electrode for H2O2 and AA was characterized by cyclic voltammetry. Under optimized experimental conditions, highly linear calibration plots were observed for both H2O2 and AA, with concentration linear ranges of 0.8 μM to 0.8 mM and 1.0 μM to 0.8 mM. Detection limits of 0.2 μM H2O2 and 0.5 μM AA were determined on the basis of the signal‐to‐noise characteristics (S/N=3) of an electropherogram. Compared with the unmodified PtME, the sensitivity was promoted in that PtNPs/PtME provided an increased effective electrode surface and high catalytic activity toward H2O2 and AA. Using this method, the added H2O2 and AA in Mizone, a kind of functional drink, were detected, and the concentration of AA was found to be 2.33 mM (n=3). The recovery rates were 95.3 % for H2O2 and 98.7 % for AA. The novel approach provided a wide linear range, low detection limit, good reproducibility and stability. It will provide a new insight into the balance of reactive oxygen species and antioxidant in biological systems.

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Redox Protein-Polymer Films for Simultaneous Determination of Ascorbic Acid and Hydrogen Peroxide

Cited by 6 publications

References 40 publications

Redox polymer covalently modified multiwalled carbon nanotube based sensors for sensitive acetaminophen and ascorbic acid detection

Redox polymer covalently modified multiwalled carbon nanotube based sensors for sensitive acetaminophen and ascorbic acid detection

Simultaneous Detection of Ascorbic Acid and Hydrogen Peroxide by Flow‐Injection Analysis with a Thin Layer Dual‐Electrode Detector

Simultaneous Determination of Peroxide Hydrogen and Ascorbic Acid by Capillary Electrophoresis with Platinum Nanoparticles Modified Micro‐disk Electrode

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