Redox-responsive functionalized hydrogel marble for the generation of cellular spheroids

Shape‐Designable Polyhedral Liquid Marbles/Plasticines Stabilized with Polymer Plates

“…Furthermore, the inner liquid can be released by disruption of the LMs via application of external stimuli, such as pH, temperature, magnetic field, and mechanical stress. [ 5 ] Thanks to these characters, researches on LMs have yielded various promising applications such as carriers of materials in microfluidics, [ 6,8–12 ] sensors, [ 13–17 ] miniature reactors for chemical and biological reactions, [ 3,7,18–24 ] and powdered pressure‐sensitive adhesives. [ 25 ]…”

Liquid marbles: review of recent progress in physical properties, formation techniques, and lab-in-a-marble applications in microreactors and biosensors

“…Cells in the liquid phase of LMs tend to self-assemble to form spheroid- and organoid-like structures, which have been observed in C6 rat gliomas, human mammary fibroblasts, and human hepatocellular carcinoma cells. 147–149 Aalders et al were the first to apply LMs to the 3D culture of induced pluripotent stem cell (iPSC)-derived cardiomyocytes and succeeded in forming cardiomyocytes in less than 24 hours (Fig. 7G).…”

Responsive Hydrogels Based on Triggered Click Reactions for Liver Cancer

“…Similarly, Ramadhan et al used a hydrogelation system based on redox response as the foundation for their hydrogel marbles (HMs). [143] To facilitate the formation of spheroid, enzymemediated hydrogelation was used to cross-link PEG-SH with thiolated gelatin to promote disulfide formation. After the scaffolds have been loaded with cells, they can be degraded under reducing conditions to retrieve the spheroids.…”