Reduced Absorption and Impaired Translocation Endows Glyphosate Resistance in <i>Amaranthus palmeri</i> Harvested in Glyphosate-Resistant Soybean from Argentina

Palma-Bautista, Candelario; Torra, Joel; García, María J.; Bracamonte, Enzo; Rojano-Delgado, Antonia M.; Cruz, Ricardo Alcántara‐de la; Prado, Rafael De

doi:10.1021/acs.jafc.8b06105

Cited by 39 publications

(35 citation statements)

References 46 publications

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“…Once the glyphosate susceptibility profile of the GSH and GRH populations via shikimate accumulation were differentiated, F 1 individuals from these populations were identified as being A. hybridus subsp. hybridus , based on the length of intron 1 of the EPSPS gene [27,28].…”

Section: Discussionmentioning

confidence: 99%

The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus

García

Palma-Bautista

Rojano-Delgado

et al. 2019

IJMS

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The introduction of glyphosate-resistant (GR) crops revolutionized weed management; however, the improper use of this technology has selected for a wide range of weeds resistant to glyphosate, referred to as superweeds. We characterized the high glyphosate resistance level of an Amaranthus hybridus population (GRH)—a superweed collected in a GR-soybean field from Cordoba, Argentina—as well as the resistance mechanisms that govern it in comparison to a susceptible population (GSH). The GRH population was 100.6 times more resistant than the GSH population. Reduced absorption and metabolism of glyphosate, as well as gene duplication of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) or its overexpression did not contribute to this resistance. However, GSH plants translocated at least 10% more 14C-glyphosate to the rest of the plant and roots than GRH plants at 9 h after treatment. In addition, a novel triple amino acid substitution from TAP (wild type, GSH) to IVS (triple mutant, GRH) was identified in the EPSPS gene of the GRH. The nucleotide substitutions consisted of ATA102, GTC103 and TCA106 instead of ACA102, GCG103, and CCA106, respectively. The hydrogen bond distances between Gly-101 and Arg-105 positions increased from 2.89 Å (wild type) to 2.93 Å (triple-mutant) according to the EPSPS structural modeling. These results support that the high level of glyphosate resistance of the GRH A. hybridus population was mainly governed by the triple mutation TAP-IVS found of the EPSPS target site, but the impaired translocation of herbicide also contributed in this resistance.

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Section: Discussionmentioning

confidence: 99%

The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus

García

Palma-Bautista

Rojano-Delgado

et al. 2019

IJMS

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“…The impaired transport from the treated leaf to meristematic growing points reduces the efficacy of systemic herbicides. 35,36 However, the high resistance levels to glyphosate can not only be explained with the association of these mechanisms. Therefore, other mechanisms such as metabolism (NTSR) and/or mutations in the EPSPS and number of copies and overexpression (TSR) could also be involved, as observed in other P. hysterophorus populations collected in Cuba, Dominican Republic 4 and Mexico.…”

Section: C-24-d and 14 C-glyphosate Absorption And Translocationmentioning

confidence: 99%

“…38 Due to this loss of affinity in the binding between the glyphosate/PEP versus EPSPS enzyme, mutation at 102 position would be unlikely to occur first or independently of Pro-106 mutations and appear generally associated with changes in the position 106. 36 However, the single mutation Thr-102-Ser was found in Tridax procumbens. 12 Partenium hysterophorus represents the third species worldwide (second broadleaf) carrying the TIPS mutation, after its previous identification in Bidens pilosa and Eleusine indica.…”

Section: Epsps Gene Sequencingmentioning

confidence: 99%

Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards

Mora

Rosario

Rojano-Delgado

et al. 2019

J. Agric. Food Chem.

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Dominican farmers have started to apply synthetic auxin herbicides (SAH) as the main alternative to mitigate the impacts of the occurrence of glyphosate resistant (GR) Parthenium hysterophorus populations in citrus orchards. A GR P. hysterophorus population survived field labeled rates of glyphosate, 2,4-D, dicamba and picloram, which showed poor control (<50%). In in vivo assays, resistance levels to glyphosate were high, and moderate to picloram, dicamba and 2,4-D. Sequencing the 5enolpyruvylshikimate-3-phosphate synthase gene revealed the double Thr-102-Ile and Pro-106-Ser (TIPS) amino acid substitution conferring resistance to glyphosate. Additionally, reduced absorption and impaired translocation contributed to this resistance. Regarding SAH, impaired 2,4-D transport and enhanced metabolism were confirmed in resistant plants. The application of malathion improved the efficacy of SAH (control >50%), showing that metabolism of these herbicides was mediated by cytochrome P450 enzymes. This study reports for the first time, multiple resistance to SAH and glyphosate in P. hysterophorus.

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“…Reduced translocation of glyphosate to meristematic sinks has been reported as the most common NTSR mechanism [78,79,80]. This mechanism has been reported in Palmer amaranth [81,82,83], horseweed [84], hairy fleabane [84], Italian ryegrass [85], rigid ryegrass [74,86], common waterhemp [73], Johnsongrass [87,88], sourgrass [89], and giant ragweed [90]. Reduction in translocation has been attributed to the evolution of a transporter that sequesters glyphosate inside the plant vacuole, thus preventing it from reaching the chloroplast [78].…”

Section: Known Ntsr Mechanisms In Weed Species For Different Herbimentioning

confidence: 99%

Non-Target-Site Resistance to Herbicides: Recent Developments

Jugulam

Shyam

2019

Plants

132

114

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Non-target-site resistance (NTSR) to herbicides in weeds can be conferred as a result of the alteration of one or more physiological processes, including herbicide absorption, translocation, sequestration, and metabolism. The mechanisms of NTSR are generally more complex to decipher than target-site resistance (TSR) and can impart cross-resistance to herbicides with different modes of action. Metabolism-based NTSR has been reported in many agriculturally important weeds, although reduced translocation and sequestration of herbicides has also been found in some weeds. This review focuses on summarizing the recent advances in our understanding of the physiological, biochemical, and molecular basis of NTSR mechanisms found in weed species. Further, the importance of examining the co-existence of TSR and NTSR for the same herbicide in the same weed species and influence of environmental conditions in the altering and selection of NTSR is also discussed. Knowledge of the prevalence of NTSR mechanisms and co-existing TSR and NTSR in weeds is crucial for designing sustainable weed management strategies to discourage the further evolution and selection of herbicide resistance in weeds.

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Reduced Absorption and Impaired Translocation Endows Glyphosate Resistance in Amaranthus palmeri Harvested in Glyphosate-Resistant Soybean from Argentina

Cited by 39 publications

References 46 publications

The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus

The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus

Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards

Non-Target-Site Resistance to Herbicides: Recent Developments

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