Reduced calcium sensitivity of dihydropyridine binding to calcium channels in spontaneously hypertensive rats.

Ebata, Hitoshi; Nishizaki, Takashi; Mitsuhashi, Takeshi; Yaginuma, T.

doi:10.1161/01.hyp.17.2.234

Cited by 15 publications

(4 citation statements)

References 44 publications

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“…Ionomycin also induced a greater increase in [Ca 2+ ]i in T‐cells of diabetic rats compared with those of control rats. Our findings can be supported by the observations of Ebata et al (52), who have reported an increased density of calcium channels in diabetic rats. Similarly, ionomycin‐induced calcium increase was higher in T‐cells of obese pups than in those of control animals.…”

Section: Discussionsupporting

confidence: 93%

n‐3 Fatty Acids Modulate T‐Cell Calcium Signaling in Obese Macrosomic Rats

Guermouche¹,

Yessoufou²,

Mokhtari-Soulimane³

et al. 2004

Obesity Research

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GUERMOUCHE, BAYA, AKADIRI YESSOUFOU, NASSIMA SOULIMANE, HAFIDA MERZOUK, KABIROU MOUTAIROU, AZIZ HICHAMI, AND NAIM AKHTAR KHAN. n-3 fatty acids modulate T-cell calcium signaling in obese macrosomic rats. Obes Res. 2004;12: 1744 -1753. Objective: We investigated the effects of a diet containing EPAX-7010, rich in PUFAs such as eicosapentaenoic acid [20:5(n-3)] and docosahexaenoic acid [22:6(n-3)], i.e., a PUFA/EPAX regimen, on T-cell activation in diabetic pregnant rats and their obese pups. Research Methods and Procedures:Mild hyperglycemia in pregnant rats was induced by intraperitoneal injection of streptozotocin on Day 5 of gestation. T-cell blastogenesis was assayed by using 3 H-thymidine, whereas intracellular free calcium concentrations ([Ca 2ϩ ]i) were measured by using Fura-2 in diabetic pregnant rats and their obese offspring. Results: Concavalin-A-stimulated T-cell proliferation was decreased in both pregnant diabetic rats and their obese pups as compared with control animals. Feeding the PUFA/ EPAX diet restored T-cell proliferation in both groups of animals. We also employed ionomycin, which at 50 nM opens calcium channels, and thapsigargin (TG), which recruits [Ca 2ϩ ]i from endoplasmic reticulum pool. We observed that ionomycin-induced increases in [Ca 2ϩ ]i in Tcells of diabetic mothers and obese offspring were greater than in those of control rats. Furthermore, feeding PUFA/ EPAX diet diminished significantly the ionomycin-evoked rise in [Ca 2ϩ ]i in diabetic and obese animals. TG-induced increases in [Ca 2ϩ ]i in T-cells of diabetic pregnant rats and their obese offspring were greater than in those of control rats. The feeding of the experimental diet significantly curtailed the TG-evoked increases in [Ca 2ϩ ]i in both diabetic and obese rats. Discussion: Together, these observations provide evidence that T-cell activation and T-cell calcium signaling are altered during gestational diabetes and macrosomia. Hence, dietary fish oils, particularly eicosapentaenoic acid and docosahexaenoic acid, may restore these T-cell abnormalities.

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Section: Discussionsupporting

confidence: 93%

n‐3 Fatty Acids Modulate T‐Cell Calcium Signaling in Obese Macrosomic Rats

Guermouche¹,

Yessoufou²,

Mokhtari-Soulimane³

et al. 2004

Obesity Research

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“…Hence, we hypothesize that the plasma membranes of T cells from SHR may have different composition than those of WKY rats. This is supported by the findings of Ebata et al (1999) who reported an increased density of L-type calcium channels in heart and brain of SHR.…”

Section: Discussionsupporting

confidence: 80%

Moringa oleifera-Rich Diet and T Cell Calcium Signaling in Spontaneously Hypertensive Rats

Attakpa

Bertin²,

Chabi³

et al. 2017

Physiol Res

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Moringa oleifera is a plant whose fruits, roots and leaves have been advocated for traditional medicinal uses. The physico-chemical analysis shows that Moringa oleifera contains more dietary polyunsaturated fatty acids (PUFA) than saturated fatty acids (SFA). The consumption of an experimental diet enriched with Moringa oleifera extracts lowered blood pressure in spontaneously hypertensive rats (SHR), but not in normotensive Wistar-Kyoto (WKY) rats as compared to rats fed an unsupplemented control diet. Anti-CD3-stimulated T cell proliferation was diminished in both strains of rats fed the Moringa oleifera. The experimental diet lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats fed the control diet. Studies of platelets from patients with primary hypertension and from SHR support the notion that the concentration of intracellular free calcium [Ca2+]i is modified in both clinical and experimental hypertension. We observed that the basal, [Ca2+]i was lower in T cells of SHR than in those of WKY rats fed the control diet. Feeding the diet with Moringa oleifera extracts to WKY rats did not alter basal [Ca2+]i in T cells but increased basal [Ca2+]i in SHR. Our study clearly demonstrated that Moringa oleifera exerts antihypertensive effects by inhibiting the secretion of IL-2 and modulates T cell calcium signaling in hypertensive rats.

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“…It is interesting that human and experimental hypertensive states other than pre‐eclampsia have also been associated to an increased density of calcium channels 41 . Similarly to what we have found in pre‐eclampsia, studies using labelled nitrendipine and PN 200 110 42 also reported an increased density and affinity of calcium channels in the vascular system of spontaneously hypertensive rats.…”

Section: Discussionsupporting

confidence: 73%

Association of circulating endothelin and noradrenaline with increased calcium‐channel binding sites in the placental bed in pre‐eclampsia

Ferreira-de-Almeida¹,

Amenta

Cardoso³

et al. 1998

BJOG

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Objective To evaluate factors contributing to both placental hypoperfusion and maternal Design Single centre, comparative study of calcium-channel density and affinity in the placental bed Setting Teaching hospital.Participants Twenty-two primigravidae in the third trimester of pregnancy: 10 with pre-eclampsia and 12 normotensive.Methods Plasma levels of endothelin-1 (by RIA) and noradrenaline (by HPLC-ED) were measured. Both pharmacological characterisation and anatomical localisation of dihydropyridinesensitive binding sites (using radioligand-binding studies and autorradiographic techniques) were determined with 3H-isradipine in placental bed tissues to determine both the density (Bmax) and the affinity (Kd) of receptor sites. Results Higher plasma levels of endothelin-1 and noradrenalin were found in women with preeclampsia compared with normotensive women. Placental bed tissues bound 3H-isradipine in a saturable, reversible time and temperature-dependent manner with very low Kd values. Study of the 3H-isradipine specificity binding included the use of several dihydropyridine displacers. In the group with pre-eclampsia the Scatchard analysis of the results showed a significant increase ( P < . Autoradiography confirmed that in the placental bed tissue of those with pre-eclampsia there was a much higher silver grain density in the arteries walls, compared with norrnotensive women.0 ConclusionsIn pre-eclampsia there is an increase in the maternal circulation of two strong vasoconstrictor factors (endothelin-1 and noradrenalin) and a sharp increase both in the density and the affinity of calcium-channel binding sites in placental bed central area. The latter may strongly contribute to the perpetuation of the uteroplacental hypoperfusion either by itself or by amplifying the local actions of circulating factors, such as endothelin-1 and noradrenalin.vasoconstriction in pre-eclampsia.of pregnant women with normotension and pre-eclampsia.

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Reduced calcium sensitivity of dihydropyridine binding to calcium channels in spontaneously hypertensive rats.

Cited by 15 publications

References 44 publications

n‐3 Fatty Acids Modulate T‐Cell Calcium Signaling in Obese Macrosomic Rats

n‐3 Fatty Acids Modulate T‐Cell Calcium Signaling in Obese Macrosomic Rats

Moringa oleifera-Rich Diet and T Cell Calcium Signaling in Spontaneously Hypertensive Rats

Association of circulating endothelin and noradrenaline with increased calcium‐channel binding sites in the placental bed in pre‐eclampsia

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