Reduced cyclic stretch, endothelial dysfunction, and oxidative stress: an ex vivo model

Thacher, Tyler; Gambillara, Veronica; Silva, Rafaela da; Silacci, Paolo; Stergiopulos, Nikolaos

doi:10.1016/j.carpath.2009.06.007

Cited by 46 publications

(35 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the stretching of an isolated vessel induces vasodilation by accelerating nitric oxide production. 26 Static stretching exercise may stimulate compression and extension in regional arteries; therefore, the reduction in peripheral arterial stiffness (faPWV) observed in this study may be related to the local mechanical effect of acute stretching exercise on peripheral arteries. 25,26 However, it is unclear whether arterial compression and extension were caused by the acute stretching exercise used in this study, and whether they resulted in acceleration of vasodilation in regional arteries.…”

Section: Discussionmentioning

confidence: 83%

“…26 Static stretching exercise may stimulate compression and extension in regional arteries; therefore, the reduction in peripheral arterial stiffness (faPWV) observed in this study may be related to the local mechanical effect of acute stretching exercise on peripheral arteries. 25,26 However, it is unclear whether arterial compression and extension were caused by the acute stretching exercise used in this study, and whether they resulted in acceleration of vasodilation in regional arteries. Acute aerobic exercise decreases both central and peripheral arterial stiffness 20,21 ; by contrast, acute stretching exercise decreases only arterial stiffness in the legs, with no change in central arterial stiffness.…”

Section: Discussionmentioning

confidence: 83%

See 1 more Smart Citation

Acute Effect of Static Stretching Exercise on Arterial Stiffness in Healthy Young Adults

Yamato

Hasegawa

Sato

et al. 2016

American Journal of Physical Medicine &Amp; Rehabilitation

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 83%

Acute Effect of Static Stretching Exercise on Arterial Stiffness in Healthy Young Adults

Yamato

Hasegawa

Sato

et al. 2016

American Journal of Physical Medicine &Amp; Rehabilitation

View full text Add to dashboard Cite

show abstract

“…Vessels typically used for these studies include segments from bovine or porcine aortae, carotid arteries, or coronary arteries [132,[143][144][145][146][147]. Human umbilical cord veins have also been used [142], and culture of isolated ECs on a de-endothelialized vessel segment has been demonstrated as well [148].…”

Section: Neonatal Rat Pulmonary Fibroblastsmentioning

confidence: 99%

“…However, if the intention is to investigate the effects of FSS and stretch independently, care must be taken to maintain the same FSS in a vessel where stretching is increased or decreased compared to the control. FSS is highly sensitive to vessel diameter, so the two stimuli are not decoupled in this approach unless the flow is altered to account for the diameter change [145,147].…”

Section: Neonatal Rat Pulmonary Fibroblastsmentioning

confidence: 99%

See 1 more Smart Citation

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

Davis

Zambrano

Anumolu

et al. 2015

Journal of Biomechanical Engineering

View full text Add to dashboard Cite

The most common cause of death in the developed world is cardiovascular disease. For decades, this has provided a powerful motivation to study the effects of mechanical forces on vascular cells in a controlled setting, since these cells have been implicated in the development of disease. Early efforts in the 1970 s included the first use of a parallel-plate flow system to apply shear stress to endothelial cells (ECs) and the development of uniaxial substrate stretching techniques (Krueger et al., 1971, "An in Vitro Study of Flow Response by Cells," J. Biomech., 4(1), pp. 31-36 and Meikle et al., 1979, "Rabbit Cranial Sutures in Vitro: A New Experimental Model for Studying the Response of Fibrous Joints to Mechanical Stress," Calcif. Tissue Int., 28(2), pp. 13-144). Since then, a multitude of in vitro devices have been designed and developed for mechanical stimulation of vascular cells and tissues in an effort to better understand their response to in vivo physiologic mechanical conditions. This article reviews the functional attributes of mechanical bioreactors developed in the 21st century, including their major advantages and disadvantages. Each of these systems has been categorized in terms of their primary loading modality: fluid shear stress (FSS), substrate distention, combined distention and fluid shear, or other applied forces. The goal of this article is to provide researchers with a survey of useful methodologies that can be adapted to studies in this area, and to clarify future possibilities for improved research methods.

show abstract

A Microfluidic Model Artery for Studying the Mechanobiology of Endothelial Cells

Kang

Whang

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

Recent vascular mechanobiology studies find that endothelial cells (ECs) convert multiple mechanical forces into functional responses in a nonadditive way, suggesting that signaling pathways such as those regulating cytoskeleton may be shared among the processes of converting individual forces. However, previous in vitro EC-culture platforms are inherent with extraneous mechanical components, which may saturate or insufficiently activate the shared signaling pathways and accordingly, may misguide EC mechanobiological responses being investigated. Here, a more physiologically relevant model artery is reported that accurately reproduces most of the mechanical forces found in vivo, which can be individually varied in any combination to pathological levels to achieve diseased states. Arterial geometries of normal and diseased states are also realized. By mimicking mechanical microenvironments of early-stage atherosclerosis, it is demonstrated that the elevated levels of the different types of stress experienced by ECs strongly correlate with the disruption of barrier integrity, suggesting that boundaries of an initial lesion could be sites for efficient disease progression.

show abstract

Reduced cyclic stretch, endothelial dysfunction, and oxidative stress: an ex vivo model

Cited by 46 publications

References 30 publications

Acute Effect of Static Stretching Exercise on Arterial Stiffness in Healthy Young Adults

Acute Effect of Static Stretching Exercise on Arterial Stiffness in Healthy Young Adults

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

A Microfluidic Model Artery for Studying the Mechanobiology of Endothelial Cells

Contact Info

Product

Resources

About