Reduced Diversity and Imbalance of Fecal Microbiota in Patients with Ulcerative Colitis

Nemoto, Hideyuki; Kataoka, Keiko; Ishikawa, Hideki; Ikata, Kazue; Arimochi, Hideki; Iwasaki, Tomonori; Ohnishi, Yoshinari; Kuwahara, Tomomi; Yasutomo, Koji

doi:10.1007/s10620-012-2236-y

Cited by 131 publications

(81 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We confirmed the results of T-RFLP by genus-specific real -time PCR with primers in Table 1. By using cloned 16S rDNA prepared from standard strains, we determined the copy number of the 16S rRNA gene from several bacteria (16,17). Recently, a new quantification system based on reverse transcription-quantitative PCR targeting of multicopy rRNA molecules was established by Matsuda et al (18).…”

Section: Culture-independent Methodsmentioning

confidence: 99%

“…Imbalance of these factors can promote disease states like inflammatory bowel disease. Since the two clinical studies described above were run at the same time, we compared the microbiota in UC patients before experimental food with those in healthy adults and found a reduced diversity and imbalance of fecal microbiota in UC patients (17). The total number of T-RF peaks was significantly lower in patients in remission than in healthy adults.…”

Section: Comparative Analysis Of the Fecal Micro-biota Between Healthmentioning

confidence: 99%

See 1 more Smart Citation

The intestinal microbiota and its role in human health and disease

Kataoka

2016

J. Med. Invest.

Self Cite

137

View full text Add to dashboard Cite

: The role of the intestinal microbiota in human health is gaining more attention since clear changes in the composition of the intestinal bacteria or environment are seen in patients with inflammatory bowel disease, allergy, autoimmune disease, and some lifestyle-related illnesses. A healthy gut environment is regulated by the exquisite balance of intestinal microbiota, metabolites, and the host's immune system. Imbalance of these factors in genetically susceptible persons may promote a disease state. Manipulation of the intestinal microbiota with prebiotics, which can selectively stimulate growth of beneficial bacteria, might help to maintain a healthy intestinal environment or improve diseased one. In this review, analytical methods for identification of intestinal bacteria and an update on the correlation of the intestinal microbiota with human health and disease were discussed by introducing our recent studies to determine the prebiotic effects of a fiber-rich food in animal model and on healthy people and patients with ulcerative colitis (UC).

show abstract

Section: Culture-independent Methodsmentioning

confidence: 99%

Section: Comparative Analysis Of the Fecal Micro-biota Between Healthmentioning

confidence: 99%

The intestinal microbiota and its role in human health and disease

Kataoka

2016

J. Med. Invest.

Self Cite

137

View full text Add to dashboard Cite

show abstract

“…The standards were stored at −80 °C, and serial dilutions (1–10 8 copies/μL) were prepared prior to each qRT-PCR assay. [35] Results for each sample were expressed as the copy number of bacterial 16S rDNA per gram of feces. Assays were performed in 96-well optical plates on the Light Cycler 480 Real-Time PCR System (Roche Diagnostics, Rotkreuz, Switzerland) in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Increased Enterococcus faecalis infection is associated with clinically active Crohn disease

Zhou¹,

Chen²,

He³

et al. 2016

Medicine

View full text Add to dashboard Cite

This study was performed to investigate the relationship between the abundance of pathogenic gut microbes in Chinese patients with inflammatory bowel disease (IBD) and disease severity.We collected clinical data and fecal samples from 47 therapy-naive Chinese patients with ulcerative colitis (UC), 67 patients with Crohn disease (CD), and 48 healthy volunteers. Bacteria levels of Fusobacterium species (spp), enterotoxigenic Bacteroides fragilis (B fragilis), enteropathogenic Escherichia coli (E coli), and Enterococcus faecalis (E faecalis) were assessed by quantitative real-time PCR (qRT-PCR). Spearman correlation coefficients were calculated to test associations between bacterial content and clinical parameters.Compared to healthy controls, the levels of both Fusobacterium spp and E faecalis were significantly increased in the feces of patients with IBD (P < 0.01). B fragilis levels were higher (P < 0.05) and E faecalis levels lower (P < 0.05) in patients with CD compared to those with UC. Increased E faecalis colonization in CD associated positively with disease activity (P = 0.015), Crohn disease activity index (CDAI; R = 0.3118, P = 0.0108), and fecal calprotectin (P = 0.016).E faecalis and Fusobacterium spp are significantly enriched in patients with IBD, and increased E faecalis infection is associated with clinically active CD.

show abstract

“…The microbial diversity was remarkably lower in IBD patients than in the healthy control group, and the reduced microbial diversity was more obvious in the active UC and active CD groups [13]. Additionally, studies of microbial profiles from UC patients revealed that these patients have lower fecal microbial diversity and lower abundance of major anaerobic bacteria ( Bacteroides and Clostridium subcluster XIVa) compared with healthy controls [23]. In another study, microarray hybridization was used to study the characteristics of the microbiota from healthy controls and children hospitalized with severe UC.…”

Section: Reduced Microbial Diversity In Ibdmentioning

confidence: 99%

Involvement of Reduced Microbial Diversity in Inflammatory Bowel Disease

Gong

Wang

et al. 2016

Gastroenterology Research and Practice

View full text Add to dashboard Cite

A considerable number of studies have been conducted to study the microbial profiles in inflammatory conditions. A common phenomenon in inflammatory bowel disease (IBD) is the reduction of the diversity of microbiota, which demonstrates that microbial diversity negatively correlates with disease severity in IBD. Increased microbial diversity is known to occur in disease remission. Species diversity plays an important role in maintaining the stability of the intestinal ecosystem as well as normal ecological function. A reduction in microbial diversity corresponds to a decrease in the stability of the ecosystem and can impair ecological function. Fecal microbiota transplantation (FMT), probiotics, and prebiotics, which aim to modulate the microbiota and restore its normal diversity, have been shown to be clinically efficacious. In this study, we hypothesized that a reduction in microbial diversity could play a role in the development of IBD.

show abstract

Reduced Diversity and Imbalance of Fecal Microbiota in Patients with Ulcerative Colitis

Cited by 131 publications

References 59 publications

The intestinal microbiota and its role in human health and disease

The intestinal microbiota and its role in human health and disease

Increased Enterococcus faecalis infection is associated with clinically active Crohn disease

Involvement of Reduced Microbial Diversity in Inflammatory Bowel Disease

Contact Info

Product

Resources

About