Reduced glutathione: Importance of specimen collection

Chavan, Sarita; Sava, Laxmayya; Saxena, Vishal; Pillai, Sandhya; Sontakke, Alka N.; Ingole, Digamber

doi:10.1007/bf02893062

Cited by 65 publications

(26 citation statements)

References 4 publications

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“…GSH is a very important antioxidant taking part in the synthesis of DNA, fixing damaged parts of DNA, inactivating toxic substances, and preventing the possible damage of free radicals (Chavan et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Melatonin is a potent modulator of antioxidative defense and cellular proliferation against aluminum toxicity in rats

Bulan¹,

Sarikaya-Unal²,

Tunalı³

et al. 2015

Turk J Biol

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Section: Discussionmentioning

confidence: 99%

Melatonin is a potent modulator of antioxidative defense and cellular proliferation against aluminum toxicity in rats

Bulan¹,

Sarikaya-Unal²,

Tunalı³

et al. 2015

Turk J Biol

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“…MDA and GSH levels and CAT and GSH-Px activities were determined in liver, kidney, and heart tissues. While MDA level was determined based on the method of Placer et al [8] , GSH level was determined based on method of Chavan et al [9] . While the method of Matkovics et al [10] was used for measurement of GSH-Px activity, Aebi [11] method was used to measure CAT activity.…”

Section: Chemical Analysismentioning

confidence: 99%

Yüksek Kalorili Bıldırcın Karma Yemlerine Deve Dikeni (Silybum marianum) Tohumu İlavesinin Yumurta Verimi, Yumurta Kalite Özellikleri, Kuluçka Randımanı ve Oksidatif Stres Parametreleri Üzerine Etkisi

Erişir¹,

Mutlu²,

Şimşek³

et al. 2016

Kafkas Univ Vet Fak Derg

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The purpose of this study was to examine the changes to occur in egg production, egg quality traits, hatchability, and oxidative stress parameters of laying quails fed with high calorie diets and effect of milk thistle (Silybum marianum) seed on these changes. A total of 75 45-day-old quails including 60 females and 15 males were used in the study. The quails were divided into 5 groups with three repetitions. 4 females and 1 male were used in each repetition. The groups of the study were arranged as following; control group (C) consuming corn-soybean based basal diet, oil group (SFO) in which 5% sun flower oil was added into basal diet, oil + milk thistle group (SFO+MT) in which 5% sun flower oil + 1% milk thistle seed was added into basal diet, syrup group (CS) in which 10% corn syrup was added into basal diet, and syrup + milk thistle group (CS+MT) in which 10% corn syrup + 1% milk thistle was added into basal diet. Total egg production was found to be significantly higher in SFO and SFO+MT groups of present study (P<0.05). Feed intake, total egg weight and feed conversion were found similar among the groups (P>0.05). The differences among the groups in albumen rate, yolk rate, dried shell rate and shape index were not statistically significant (P>0.05). The best yolk colour was obtained in SFO+MT and CS groups (P<0.01). There were no statistically differences among the groups in hatchability, hatchability of fertile and embrionic mortality (P>0.05), but the difference in examined fertility were statistically significant (P<0.05). Malonyl dialdehyde (MDA) values of blood, liver and heart tissues were similar among the groups of study (P>0.05). MDA level of kidney significantly increased in CS group, however addition of milk thistle seed into diet was reduced MDA level of kidney in CS+MT group (P<0.01). High calorie diets caused changes in antioxidant system in blood and tissues. Consequently, high-calorie basal diets especially addition of SFO improved only egg production and fertility rate. However feeding quails with corn syrup had significantly higher lipid peroxidation in kidney. Supplementation of milk thistle seed into CS group may protect kidney against free radical damage. Keywords ÖzetBu araştırmada yüksek kalorili yemlerle beslenen yumurtacı bıldırcınlarda yumurta verimi, yumurta kalite özellikleri, kuluçka performansı ve oksidatif stres parametrelerinde meydana gelebilecek değişimler ve bu değişimler üzerine deve dikeni (Silybum marianum) tohumunun etkisinin incelenmesi amaçlanmıştır. Araştırmada, 45 günlük yaşta 60 adet dişi, 15 adet erkek olmak üzere toplam 75 adet bıldırcın kullanılmıştır. Araştırma 5 grup ve her grupta 3 tekerrürden oluşturulmuştur. Her bir tekerrürde 4 dişi ve 1 erkek bıldırcın kullanılmıştır. Deneme grupları; mısır-soya esasına dayalı temel karma yemi tüketen kontrol grubu (C), temel diyete % 5 ay çiçeği yağı ilave edilen yağ grubu (SFO), temel diyete %5 ay çiçeği yağı + %1 deve dikeni ilave edilen yağ + deve dikeni grubu (SFO+MT), temel diyete %10 mısır şurubu ilave ...

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“…Glutathione was determined in total blood using the method described by Chavan et al [12]. This method is based on reductive cleavage of 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) reagent by the sulfhydryl group of reduced glutathione to yield a yellow color, measured at 412 nm.…”

Section: Determination Of Glutathione Malondialdhyde and Nitric Oxidementioning

confidence: 99%

Alpha-Lipoic Acid Improves Subclinical Left Ventricular Dysfunction in Asymptomatic Patients with Type 1 Diabetes

Hegazy¹,

Tolba²,

Mostafa³

et al. 2013

Rev Diabet Stud

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■ Abstract BACKGROUND:Oxidative stress plays an important role in the development of diabetic cardiomyopathy. Alpha-lipoic acid (ALA) is a powerful antioxidant that may have a protective role in diabetic cardiac dysfunction. AIM: We investigated the possible beneficial effect of alpha-lipoic acid on diabetic left ventricular (LV) dysfunction in children and adolescents with asymptomatic type 1 diabetes (T1D). SUB-JECTS AND METHODS: Thirty T1D patients (aged 10-14) were randomized to receive insulin treatment (n = 15) or insulin plus alpha-lipoic acid 300 mg twice daily (n = 15) for four months. Age and sex matched healthy controls (n = 15) were also included. Patients were evaluated with conventional 2-dimensional echocardiographic examination (2D), pulsed tissue Doppler (PTD), and 2-dimensional longitudinal strain echocardiography (2DS) before and after therapy. Glutathione, malondialdhyde (MDA), nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), Fas ligand (Fas-L), matrix metalloproteinase 2 (MMP-2), and troponin-I were determined and correlated to echocardiographic parameters. RESULTS: Diabetic patients had significantly lower levels of glutathione and significantly higher MDA, NO, TNF-alpha, Fas-L, MMP-2, and troponin-I levels than control subjects. The expression of transforming growth factor beta (TGF-beta) mRNA in peripheral blood mononuclear cells was also increased in diabetic patients. Significant correlations of mitral e'/a' ratio and left ventricular global peak systolic strain with glutathione, MDA, NO, TNF-alpha, and Fas-L were observed in diabetic patients. Alpha-lipoic acid significantly increased glutathione level and significantly decreased MDA, NO, TNF-alpha, Fas-L, MMP-2, troponin-I levels, and TGF-beta gene expression. Moreover, alphalipoic acid significantly increased mitral e'/a' ratio and left ventricular global peak systolic strain in diabetic patients. CONCLUSION: These findings suggest that alpha-lipoic acid may have a role in preventing the development of diabetic cardiomyopathy in type 1 diabetes.

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Reduced glutathione: Importance of specimen collection

Cited by 65 publications

References 4 publications

Melatonin is a potent modulator of antioxidative defense and cellular proliferation against aluminum toxicity in rats

Melatonin is a potent modulator of antioxidative defense and cellular proliferation against aluminum toxicity in rats

Yüksek Kalorili Bıldırcın Karma Yemlerine Deve Dikeni (Silybum marianum) Tohumu İlavesinin Yumurta Verimi, Yumurta Kalite Özellikleri, Kuluçka Randımanı ve Oksidatif Stres Parametreleri Üzerine Etkisi

Alpha-Lipoic Acid Improves Subclinical Left Ventricular Dysfunction in Asymptomatic Patients with Type 1 Diabetes

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