Reduced Lipopolysaccharide (LPS)-Induced Nitric Oxide Production in Peritoneal Macrophages and Inhibited LPS-Induced Lethal Shock in Mice by a Sugar Cane (<i>Saccharum officinarum</i>L.) Extract

Hikosaka, Kenji; Koyama, Yukari; Motobu, Maki; Yamada, Manabu; Nakamura, Kikuyasu; Koge, Kenji; Shimura, Kameo; Isobe, Takashi; Tsuji, Naotoshi; Kang, Chung-Boo; Hayashidani, Hideki; Wang, Pichao; Matsumura, Masatoshi; Hirota, Yoshikazu

doi:10.1271/bbb.60242

Cited by 12 publications

(10 citation statements)

References 27 publications

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“…In this work, it was shown that molasses treatment exhibited a suppressive effect on NO production and these results are in concordance with Hikosaka et al (2006) who found that sugarcane extract reduced lipopolysaccharide-induced NO production in the peritoneal macrophages of rats. Moreover, molasses might have decreased the inflammatory responses induced by toxicity (Rahiman, 2011), lowering NO level. A marked decrease in the serum of testosterone was observed post-radiation or Mn-toxicity as compared to control animals.…”

Section: Discussionsupporting

confidence: 91%

Ameliorative Effect of Sugarcane (Blackstrap) Molasses against Gamma Radiation or Manganese- Induced Testicular Toxicity in Male Albino Rats

et al. 2019

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S UGARCANE molasses is a potent antioxidant. Radiation and excess manganese are toxins causing a wide range of adverse effects that can be severe and induce tissues damage to mammals. In an attempt to minimize these effects, the present study aims to evaluate the mitigating effect of molasses on radiation or manganese toxicity-induced testicular tissue damage. Rats were whole-body gamma-irradiated with a single dose of 6Gy, or administered MnCl 2 4H 2 O in a single intraperitoneal injection (100mg/kg body weight). The results showed that molasses treatment (200mg/ 100g body weight/day) for seven days post-radiation or manganese toxicity induced a marked amelioration in the oxidative stress biomarkers of serum and testicular tissues of rats manifested by decreased levels of total nitrate/nitrite (NOx) and malondialdehyde (MDA) associated with an elevation in the total antioxidant capacity (TAC). Molasses treatment has induced also a significant elevation in the mitochondrial cytochrome c oxidase (COX). Marked improvement in the level of serum testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and glucose was also observed. Moreover, molasses was effective in modulating the concentration levels of copper (Cu), iron (Fe), zinc (Zn), manganese (Mn) and selenium (Se) in testicular tissue and epididymis. Additionally, the epididymal sperm count, as well as motility and viability percentage had markedly increased in rats treated with molasses, whereas there was a significant decrease in the number of sperms with abnormal morphology. In conclusion, sugarcane molasses can be considered as a new natural mitigating agent on the testicular injury induced by radiation or Mn-toxicity.

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Section: Discussionsupporting

confidence: 91%

Ameliorative Effect of Sugarcane (Blackstrap) Molasses against Gamma Radiation or Manganese- Induced Testicular Toxicity in Male Albino Rats

et al. 2019

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“…Other studies on chickens indicate that SCE has a protective effect against Eimeria tenella (El‐Abasy et al , ). The effect of SCE on endotoxic shock has also been reported in mice (Hikosaka et al , ). Our recent report also indicates that SCE can up‐regulate porcine immunity by enhancing natural killer cell cytotoxicity and can decrease injury caused by pseudorabies virus infection (Lo et al , , ).…”

Section: Introductionmentioning

confidence: 77%

“…These functional enhancements in neutrophil activity were also apparent in the reduced number of bacteria in the spleen, the reduced number of hepatic lesions, and the improved survival of SCE‐treated mice compared with control mice. Additionally, it has been reported that SCE can improve the resistance of mice to endotoxin treatment (Hikosaka et al , ; Motobu et al , ), suggesting that the scavenger activity of reactive oxygen intermediates and the inhibition of inflammatory mediators by polyphenol or SCE may contribute to the decrease in the number of hepatic lesions caused by S. typhimurium endotoxin (Murakami et al , ). Therefore, the increased leukocyte numbers, the enhanced biological activity of neutrophils, and the resistance to endotoxic shock may be reflected in a lower number of hepatic lesions and the higher survival rates of SCE‐treated mice.…”

Section: Discussionmentioning

confidence: 99%

“…Sugar cane extract (SCE) is a natural product that has a wide range of biological effects, including immunostimulation (El‐Abasy et al , ) and anti‐inflammatory activity (Hikosaka et al , ). One study showed that SCE was able to restore immunity in immunosuppressed chickens treated with cyclophosphamide or X‐ray radiation (Amer et al , , ; El‐Abasy et al , ).…”

Section: Introductionmentioning

confidence: 99%

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Immunostimulation of Sugar Cane Extract on Neutrophils to Salmonella typhimurium Infection in Mice

Chen

Liao

et al. 2011

Phytotherapy Research

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The aim of this study was to evaluate the immunomodulatory effects of sugar cane extract (SCE) on the biological activities of neutrophils in mice. Six-week-old BALB/c mice were fed 1250 mg/kg of SCE once. The generation, migration and biological functions of neutrophils and the survival rates of the mice in response to Salmonella typhimurium infection were evaluated. The results show that the numbers of both bone marrow cells and neutrophils were significantly increased in response to SCE administration (p < 0.05) compared with controls. The migration, phagocytosis and H₂O₂ generation of neutrophils were all significantly enhanced in SCE-treated mice (p < 0.05). After challenge with S. typhimurium (lethal dose, 50% (LD₅₀), SCE-treated mice had a 19.2% higher survival rate and milder hepatic lesions than the controls. Additionally, fewer invasive bacteria were recovered from the spleens of SCE-treated mice. In conclusion, our results suggest that SCE has a positive regulatory effect on the biological function of mouse neutrophils that may increase host resistance against bacterial infections.

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“…Macrophage cells were stimulated with LPS for 1 h to produce hydrogen peroxide in the Krebs-Ringer buffer as a culture medium. 9,19,20 The level of hydrogen peroxide in the Krebs-Ringer buffer was measured by adding chemiluminescent micelles to the buffer and measuring the emission intensity. Figure 8 shows the chemiluminescent intensity of Krebs-Ringer buffer using micelles encapsulating diphenyl peroxalate.…”

mentioning

confidence: 99%

Detection of Hydrogen Peroxide in vitro and in vivo Using Peroxalate Chemiluminescent Micelles

Lee¹,

Hwang²,

Yoo³

et al. 2011

Bulletin of the Korean Chemical Society

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Hydrogen peroxide plays a key role as a second messenger in the normal cellular signaling but its overproduction has been implicated in various life-threatening diseases. Peroxalate chemiluminescence is the light emission from a three component reaction between peroxalate, hydrogen peroxide and fluorophores. It has proven great potential as a methodology to detect hydrogen peroxide in physiological environments because of its excellent sensitivity and specificity to hydrogen peroxide. We developed chemiluminescent micelles composed of amphiphilic polymers, peroxalate and fluorescent dyes to detect hydrogen peroxide at physiological concentrations. In this work, we studied the relationship between the chemiluminescence reactivity and stability of peroxalate by varying the substitutes on the aryl rings of peroxalate. Alkyl substitutes on the aryl ring of peroxalate increased the stability against water hydrolysis, but diminished the reactivity to hydrogen peroxide. Chemiluminescent micelles encapsulating diphenyl peroxalate showed significantly higher chemiluminescence intensity than the counterpart encapsulating dimethylphenyl or dipropylphenyl peroxalate. Diphenyl peroxalate-encapsulated micelles could detect hydrogen peroxide generated from macrophage cells stimulated by lipopolysaccharide (LPS) and image hydrogen peroxide generated during LPS-induced inflammatory responses in a mouse.

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Reduced Lipopolysaccharide (LPS)-Induced Nitric Oxide Production in Peritoneal Macrophages and Inhibited LPS-Induced Lethal Shock in Mice by a Sugar Cane (Saccharum officinarumL.) Extract

Cited by 12 publications

References 27 publications

Ameliorative Effect of Sugarcane (Blackstrap) Molasses against Gamma Radiation or Manganese- Induced Testicular Toxicity in Male Albino Rats

Ameliorative Effect of Sugarcane (Blackstrap) Molasses against Gamma Radiation or Manganese- Induced Testicular Toxicity in Male Albino Rats

Immunostimulation of Sugar Cane Extract on Neutrophils to Salmonella typhimurium Infection in Mice

Detection of Hydrogen Peroxide in vitro and in vivo Using Peroxalate Chemiluminescent Micelles

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