Reduced Locomotor Activity of Rats Made Histidinemic by Injection of Histidine

Abstract

Acute histidinemia was provoked in 30-d-old male Wistar rats by injecting intraperitoneally either histidine alone (0.5 mg/g body wt) or histidine (0.25 mg/g body wt) plus the histidase inhibitor nitromethane (0.73 mg/g body wt). Histidase activity was approximately 90% inhibited in rats receiving nitromethane. Serum histidine in both groups reached levels similar to those of histidinemic patients. Rats were subjected to the open field behavioral task, and the number of rearings and crossings were counted. A consistently lower locomotor activity was observed in the histidinemic rats. It is proposed that reduced locomotor activity and its relationship to psychomotor development should be investigated in histidinemic children.

Effects of histidine and imidazolelactic acid on various parameters of the oxidative stress in cerebral cortex of young rats

“…However, the pathomechanisms involved in the brain injury in these patients are yet not established. In this context, we have previously demonstrated that histidine administration provokes learning deficits in rats (Dutra-Filho et al, 1989), suggesting that the amino acid probably provokes alterations in the brain leading to cognitive deficit. Table 2 In vitro effect of l-␤-imidazolelactic acid on antioxidant enzyme activities in cerebral cortex homogenates from young rats l-␤-Imidazolelactic acid concentration (mM) Since oxidative stress is involved in the neuropathology of various neurodegenerative disorders, including Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, and others (Halliwell, 2001) and considering that pro-oxidant properties have been demonstrated for histidine in various tissues but not in brain, in the present study we investigated the role of l-histidine on various parameters of oxidative stress in cerebral cortex of young rats.…”

Undernutrition during the preweaning period changes calcium ATPase and ADPase activities of synaptosomal fractions of weanling rats

“…They were maintained since birth on a 200 g protein/kg laboratory chow (Germani, Porto Alegre, RS, Brazil; for details see Dutra-Filho et al 1989).…”