Reduced number and activity of circulating endothelial progenitor cells in patients with idiopathic pulmonary arterial hypertension

Abstract

Our observations indicated that EPC numbers and functional capacity were impaired in patients with IPAH, which might not only give potential insight into the pathophysiological mechanisms but also might be useful for identifying suitable therapeutic targets in these patients.

Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease

“…A volume of 100 μl of peripheral blood was obtained and incubated for 30 min at 4 °C with antihuman-KDR-PE (R&D Systems, Minneapolis, USA) and antihuman-AC133-APC (eBioscence, San Diego, USA). The suspension was collected and incubated with fluorescence-activated cell sorter (FACS) lysing solution for 10~15 min Zhu et al, 2008). FACS-Calibur instrument (Becton-Dickson, New Jersey, USA) was used to analyze cell surface marker proteins.…”

“…Several clinical studies have also demonstrated that hypoadiponectinemia correlated with endothelial dysfunction in peripheral arteries and CAD (Iwashima et al, 2006a;2006b; Journal of Zhejiang University SCIENCE B ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) www.zju.edu.cn/jzus; www.springerlink.com E-mail: jzus@zju.edu.cn Otake et al, 2008). Endothelial dysfunction is an early pivotal event in the development, progression, and manifestation of atherosclerosis and ultimately represents an imbalance between the magnitude of injury process and repair capacity (Zhu et al, 2008). Previous studies suggested that circulating endothelial progenitor cells (EPCs) participate in this endothelial repair process (Hill et al, 2003;Zhu et al, 2008).…”

“…Endothelial dysfunction is an early pivotal event in the development, progression, and manifestation of atherosclerosis and ultimately represents an imbalance between the magnitude of injury process and repair capacity (Zhu et al, 2008). Previous studies suggested that circulating endothelial progenitor cells (EPCs) participate in this endothelial repair process (Hill et al, 2003;Zhu et al, 2008).…”

“…EPCs have the capacity of homing from bone marrow, and circulate, proliferate, and differentiate into mature endothelial cells, but they have neither acquired characteristics of mature endothelial markers, nor formed a lumen (Asahara et al, 1997;Zhu et al, 2008). EPCs coexpress hematopoietic stem or progenitor cell markers (CD34 or AC133) and endothelial surface markers (VE-Cadherin or VEGFR-2) (Peichev et al, 2000;Gill et al, 2001;Zhu et al, 2008).…”

“…EPCs coexpress hematopoietic stem or progenitor cell markers (CD34 or AC133) and endothelial surface markers (VE-Cadherin or VEGFR-2) (Peichev et al, 2000;Gill et al, 2001;Zhu et al, 2008). Laboratory evidence suggests that these precursor cells participate in the processes of postnatal neovascularization and re-endothelialization (Asahara et al, 1999;Hill et al, 2003).…”