Reduced Skin T-Cell Receptor Diversity in Large Cell Transformed Mycosis Fungoides

Gleason, Laura; Talasila, Sahithi; Tekmen, Volkan; Nikbakht, Neda

doi:10.1016/j.jid.2023.03.1683

Cited by 3 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the complex nature of analyzing the immune repertoire comprised of both malignant and benign T-cells, there are limited peer-reviewed articles that address and define the T-cell repertoire in MF. However, a preliminary study suggests that changes in T-cell repertoire diversity may be related to the transformation event in large cell transformed MF and consequently contribute to its aggressive disease course ( 36 ). Considering the influence of the T-cell repertoire on disease progression in other malignancies, the utilization of HTS-TCR may further elucidate the role of the T-cell repertoire in MF.…”

Section: Utility Of Immunosequencing In Skinmentioning

confidence: 99%

“…By providing the precise genetic sequences and absolute abundance of CDR3s, HTS-TCR offers a high-resolution view of the immune repertoire. Specifically, HTS-TCR data can be used to determine T-cell repertoire diversities in blood and skin (36). In addition, HTS can assess the T-cell repertoire overlap between blood and skin or various skin lesions over time, as demonstrated by Joffe et al (58).…”

Section: Advancing Ctcl Research With Hts-tcrmentioning

confidence: 99%

See 1 more Smart Citation

Immunosequencing applications in cutaneous T-cell lymphoma

Mandel,

Gleason,

Joffe

et al. 2023

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Immunosequencing has emerged as a newer clinical test for assessment of T-cell clonality in the blood and skin of cutaneous T-cell lymphoma (CTCL) patients. Utilization of immunosequencing, also known as high-throughput sequencing of the T-cell receptor (HTS-TCR), enables identification and quantification of the precise genetic signature of dominant T-cell clones. Although immunosequencing is more sensitive than commonly used methods such as polymerase chain reaction (PCR) paired with capillary electrophoresis or flow cytometry, it remains underutilized for CTCL management. Nonetheless, incorporation of HTS-TCR in clinical practice offers distinct advantages compared to other molecular analyses that may improve diagnostic evaluation, prognostication, and disease monitoring in CTCL. The objective of this comprehensive review is to provide a thorough explanation of the application of immunosequencing in the context of CTCL. We describe the significance of T-cell clonality and the methods used to detect it, including a detailed comparison between PCR paired with capillary electrophoresis and HTS-TCR. The utilization of immunosequencing in the blood and skin of CTCL patients is discussed in depth, specifically outlining how HTS-TCR can assist in diagnosing CTCL, predicting outcomes, and tracking disease progression. Finally, we address the potential applications of immunosequencing in clinical management and research as well as the novel challenges it presents.

show abstract

Section: Utility Of Immunosequencing In Skinmentioning

confidence: 99%

Section: Advancing Ctcl Research With Hts-tcrmentioning

confidence: 99%

Immunosequencing applications in cutaneous T-cell lymphoma

Mandel,

Gleason,

Joffe

et al. 2023

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

show abstract

“… 1 Even in early stages and in the absence of blood involvement, changes in the blood T-cell receptor (TCR) repertoire have been observed in patients with MF. 2 , 3 , 4 , 5 …”

mentioning

confidence: 99%

“…ImmunoSEQ Analyzer provided T-cell repertoire overlap metric (Morisita index) and diversity measure (Simpson clonality score). 3 , 14 , 15 , 16 TTST was calculated as the time from initial diagnosis to the initiation of the first systemic therapy.…”

mentioning

confidence: 99%