Reduced susceptibility to quinupristin/dalfopristin in Enterococcus faecium in Greece without prior exposure to the agent

Karanika, M; Prati, A.; Kiritsi, M.; Spiliopoulou, Iris; Neonakis, Ioannis K.; Anifantaki, M.; Petinaki, Efthimia

doi:10.1016/j.ijantimicag.2007.08.006

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…A investigation from Japan reported that none was resistant to Q/D while 28 (17.6 %, 28/159) were intermediate resistant to this antimicrobial agent (MIC = 2 mg/L) among 159 E. faecium isolates from clinical specimens in a Japanese hospital from 1997 to 2006 [ 21 ]. A study from Greek reported that 250 of 865 (28.9 %) E. faecium isolates from patients of eight Greek hospitals between 2005 and2006 were intermediate-resistant to Q/D (MICs = 1.5–4 mg/L) [ 22 ]. In another report, all 60 primary clinical isolates of E. faecium with resistance to glycopeptides were fully susceptible to Q/D, with MIC50 and MIC90 values of 1.0 mg/L and 1.5 mg/L [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Characteristic of Enterococcus faecium clinical isolates with quinupristin/dalfopristin resistance in China

Wang

Guo

et al. 2016

BMC Microbiol

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BackgroundQuinupristin/dalfopristin (Q/D) is a valuable alternative antibiotic to vancomycin for the treatment of multi-drug resistant Enterococcus faecium infections. However, resistance to Q/D in E. faecium clinical isolates and nosocomial dissemination of Q/D-resistant E. faecium have been reported in several countries and should be of concern.ResultsFrom January 2012 to December 2015, 911 E. faecium clinical isolates were isolated from various specimens of inpatients at the first Affiliated Hospital of Wenzhou Medical University located in Wenzhou, east China. Of 911 E. faecium clinical isolates, 9 (1.0 %, 9/911) were resistant to Q/D, with the Q/D MIC values of 64 mg/L(1), 32 mg/L(1), 16 mg/L(3), 8 mg/L(1) and 4 mg/L(3) determined by broth microdilution. All Q/D-resistant isolates were susceptible to vancomycin, tigecycline and teicoplanin but resistant to penicillin, ampicillin and erythromycin. vatE was only found in one Q/D-resistant E. faecium isolate while vatD was not detected in any of the isolates tested. 8 of 9 Q/D-resistant E. faecium isolates were found be positive for both ermB and msrC. The combinations of Q/D resistance determinants were ermB-msrC (7 isolates) and ermB-msrC-vatE (one isolate). ST78, ST761, ST94, ST21 and ST323 accounted for 4, 2, 1, 1 and 1 isolate, respectively, among which ST78 was the prevalent ST.ConclusionQ/D-resistant E. faecium clinical isolates were first described in China. Carriage of vatE, ermB and msrC was responsible for Q/D resistance.

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Section: Resultsmentioning

confidence: 99%

Characteristic of Enterococcus faecium clinical isolates with quinupristin/dalfopristin resistance in China

Wang

Guo

et al. 2016

BMC Microbiol

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“…The QD resistance rate in our clinical isolates appeared not to be associated with the recent use of QD in the hospital for the treatment of VRE, as was reported previously . In the USA and Greece, QD resistance emerged even before its commercial use was reported Karanika et al, 2008). These results suggest that the use of QD may not be the sole reason for the high rate of resistance and that there may be other reasons.…”

Section: Discussionmentioning

confidence: 99%

Comparison of genetic epidemiology of vancomycin-resistant Enterococcus faecium isolates from humans and poultry

Cha

Jung

Lee

et al. 2012

Journal of Medical Microbiology

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2 (i)-ro, Osong-eup, Cheongwon-gun, Chungcheongbuk-do 363-951, Republic of Korea This study was conducted to investigate the molecular characteristics and genetic relatedness of vancomycin-resistant Enterococcus faecium (VREF) isolates obtained from humans and poultry in Korea. A total of 147 VREF isolates from humans (71 clinical isolates) and poultry (76 isolates) in Korea were compared with respect to their antibiotic susceptibilities, organization of the Tn1546 transposon element, detection of virulence genes (esp and hyl), pulsed-field gel electrophoresis (PFGE) typing and multilocus sequence typing (MLST). All of the human and poultry isolates had the vanA gene and 11.3 % (8/71) of the clinical isolates showed the VanB phenotype/vanA genotype. PCR mapping of the Tn1546 elements was different for isolates of the two groups: human isolates were classified into five transposon types, whereas all poultry isolates were identical to Tn1546 of E. faecium strain BM4147. The esp gene was detected in both human (93.0 %, 66/71) and poultry (26.3 %, 20/76) isolates, as was the hyl gene (human isolates: 80.3 %, 57/71; poultry isolates: 26.3 %, 20/76). Using MLST, the 71 human isolates could be divided into ten sequence types (STs) belonging to clonal complex (CC) 17 (except for one singleton). The 76 poultry isolates were categorized into 14 STs and 88.2 % (67/76) of the poultry isolates belonged to CC26. PFGE typing of the human isolates demonstrated diverse PFGE profiles among the strains. However, the PFGE patterns of the poultry isolates were possibly related to the strains collected from individual farms. These data suggest that epidemic clonal groups of human and poultry VREFs in Korea have evolved through different evolutionary processes.

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“…The presence of resistance genes vanABCDE, mecA and sat was determined as markers for glycopeptide, ␤-lactam and dalfopristin resistance, respectively [3,8]. Clonality of isolates was assessed by pulsed-field gel electrophoresis (PFGE) after digestion of chromosomal DNA with SmaI [3].…”

Section: Molecular Methodsmentioning

confidence: 99%

In vitro activity of daptomycin against Gram-positive cocci: the first multicentre study in Greece

Malli

Spiliopoulou

Kolonitsiou

et al. 2008

International Journal of Antimicrobial Agents

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Reduced susceptibility to quinupristin/dalfopristin in Enterococcus faecium in Greece without prior exposure to the agent

Cited by 10 publications

References 11 publications

Characteristic of Enterococcus faecium clinical isolates with quinupristin/dalfopristin resistance in China

Characteristic of Enterococcus faecium clinical isolates with quinupristin/dalfopristin resistance in China

Comparison of genetic epidemiology of vancomycin-resistant Enterococcus faecium isolates from humans and poultry

In vitro activity of daptomycin against Gram-positive cocci: the first multicentre study in Greece

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