Reducing properties, energy efficiency and carbohydrate metabolism in hyperhydric and normal carnation shoots cultured in vitro: a hypoxia stress?

Saher, Shady; Fernández‐García, Nieves; Piqueras, Abel; Hellín, E.; Olmos, Enrique

doi:10.1016/j.plaphy.2005.05.006

Cited by 40 publications

(28 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Saher et al (2005aSaher et al ( , 2005b) studied a mechanism of hyperhydricity, discovering that different pectin methyl esterase (PME) activities influence structural changes related to hyperhydricity in micropropagated carnation plants. Hyperhydric leaves of carnations adapted to hypoxia stress conditions through both oxidative pentose phosphate and fermentative pathway induction.…”

Section: Effects Of Pgrs On the Morphogenesis Of Hyperhydric Leaves Amentioning

confidence: 99%

Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro

Yang

Qin

Sun

et al. 2012

Not Bot Hort Agrobot Cluj

View full text Add to dashboard Cite

An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate plantlets, the stem segments of which were cultured for shoot proliferation and plantlet multiplication. The results showed that the leaf base was the optimal explant, as compared to both the middle and the top of leaves, for shoot formation. The highest shoot induction of 88.9% was observed on MS medium supplemented with 0.6 mg/l TDZ and 0.1 mg/l NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, and then into hyperhydric plantlets after an additional 10 days. The hyperhydric plantlets reversed to normal plantlets when plant growth regulators were removed from culture medium. Further, stem segments from reversed plantlets were used for shoot regeneration and root induction. Optimal shoot regeneration was obtained in MS medium containing 0.6 mg/l TDZ with 0.1 mg/l NAA. Root induction and root mean number were all higher on auxin-free medium than on medium containing auxins.

show abstract

Section: Effects Of Pgrs On the Morphogenesis Of Hyperhydric Leaves Amentioning

confidence: 99%

Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro

Yang

Qin

Sun

et al. 2012

Not Bot Hort Agrobot Cluj

View full text Add to dashboard Cite

show abstract

“…The mechanism involved in lipid peroxidation during hypoxia is not clear although it is known that various cellular activities can generate H 2 O 2 in plants. Saher et al [ 59 ] found induction of the oxidative pentose phosphate and fermentative pathways in carnation hyperhydric leaves. According to their opinion, hypoxia stress was the main factor affecting metabolism of hyperhydric leaves.…”

Section: Hypoxia Stress and Lipid Peroxidationmentioning

confidence: 99%

“…In hyperhydric shoots of Prunus avium , H 2 O 2 was accumulated due to increased SOD activity and decreased activities of POX, APX, CAT, DHAR, MDHAR and GR [ 61 ]. Using bottom cooling to prevent hyperhydricity in micropropagated Dianthus decreased H 2 O 2 production, lipid peroxidation (MDA content), and SOD and CAT activities [ 59 ]. Addition of rare earth elements La, Ce and Nd into medium reduced hyperhydricity in Lepidium meyeni shoots and enhanced activities of POD, CAT, APX, SOD, MDHAR, and GR [ 72 ].…”

Section: Reactive Oxygen Species (Ros) and Antioxidant Enzyme Activitiesmentioning

confidence: 99%

Biochemical and Physiological Aspects of Hyperhydricity in Liquid Culture System

Dewir

Indoliya

Chakrabarty

et al. 2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

View full text Add to dashboard Cite

Large-scale liquid cultures and automation have proven the potential to resolve manual handling of in vitro cultures at various stages and decreases production cost. However, hyperhydricity is a major problem during in vitro culture of many crops in liquid culture systems. The environment inside culture vessel normally used in plant micropropagation is characterized by high humidity, limited gaseous exchange between the internal atmosphere of the culture vessel and its surrounding environment, and the accumulation of ethylene, conditions that may induce physiological disorders. Hyperhydricity is a disorder of tissue-cultured plants where leaves become translucent and stems swollen, distorted and brittle. Although numerous hypotheses have been put forward to explain hyperhydricity but there is still a lack of knowledge about the nature of signals responsible for hyperhydricity and the metabolic processes which are affected by its development. The concept of stress in relation to hyperhydricity is not completely established. Therefore, it remains diffi cult to assume that hyperhydric tissues are stressed. Previous studies argued that abnormal morphology observed in hyperhydricity could be attributed to changes occurring at cellular level due to the modifi cations of membrane composition or DNA content. In order to understand stress and morphological responses in hyperhydric tissues, in the present article, we are reviewing different biochemical and physiological mechanisms of hyperhydricity in several plant species.

show abstract

“…A água que forma uma camada em volta das células pode limitar a difusão do oxigênio para elas, causando hipoxia, e induzir a formação de peróxido de hidrogênio (H 2 O 2 ) em níveis tóxicos, resultando em estresse oxidativo (SAHER et al, 2004). A hipoxia, à medida que afeta o processo respiratório, reduz a disponibilidade de ATP produzido nas mitocôndrias por meio da fosforilação oxidativa (SAHER et al, 2005a). A avaliação do metabolismo energético de partes aéreas hiperídricas tem demonstrado também uma queda na produção de energia química na forma de nucleotídeos de piridina, NADPH e NADH, além de ATP.…”

Section: Aspectos Morfológicosunclassified

“…Essa diminuição no metabolismo energético está associada a um menor teor de clorofila e a uma menor atividade de enzimas da via oxidativa das pentoses-fosfato (OPP) e da glicólise (FRANCK et al, 2001). Medidas da fluorescência de clorofila permitiram constatar uma discreta redução do processo fotoquímico do fotossistema II (FS-II) de folhas hiperídricas, quando comparadas com folhas normais, indicando que a menor capacidade fotossintética deve-se mais propriamente ao menor teor de clorofila do que a uma disfunção do aparato fotossintético (FRANCK et al, 2001;SAHER et al, 2005a). O reduzido conteúdo de celulose (GASPAR, 1991;MAJADA et al, 2001) ocorre em função da baixa relação C/N -que favorece a síntese de aminoácidos em detrimento da síntese do polissacarídeo -e da alta atividade de peroxidases básicas, que está relacionada com um elevado catabolismo de auxinas (JOYCE et al, 2003).…”

Section: Aspectos Morfológicosunclassified