Reduction effect of the selected chemical and physical treatments to reduce L. monocytogenes biofilms formed on lettuce and cabbage

Srey, Sokunrotanak; Park, Shin Young; Jahid, Iqbal Kabir; Ha, Sang‐Do

doi:10.1016/j.foodres.2014.03.067

Cited by 61 publications

(29 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…33 Our previous studies demonstrated that high-voltage DBD ACP in conjunction with treatment of contaminated objects inside sealed packages and following post treatment storage was very effective for inactivation of bacteria in their planktonic form, reducing the numbers within seconds. 31 However, bacterial biofilms, as a predominant mode of microbial growth, 34 represent major challenges in industrial and health care settings due to their increased mechanical stability and antimicrobial resistance. 35 Therefore, in the present study, the potential of ACP to inactivate bacterial pathogens was investigated against challenge P. aeruginosa biofilms, as one of the major causes of healthcare-associated infections.…”

Section: Discussionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

View full text Add to dashboard Cite

ABSTRACT:In recent years, atmospheric cold plasma (ACP) has been widely investigated for potential application as an alternative decontamination technology in biomedical and healthcare sectors. In this study, the antimicrobial efficacy of ACP against Pseudomonas aeruginosa biofilms was investigated. The 48-h biofilms were treated inside sealed polypropylene containers with a high-voltage dielectric barrier discharge (DBD) ACP (80 kV RMS ) and subsequently stored for 24 h at room temperature. Treatment for 60 s by either the direct or indirect mode of ACP exposure (inside or outside plasma discharge, respectively) reduced bacterial populations by an average of 5.4 log cycles from an initial 6.6 log 10 CFU/mL. Increasing the treatment time from 60 s to 120 s and 300 s reduced biofilms to undetectable levels. According to XTT assay (a metabolic activity assay), an extended treatment time of 300 s was necessary to reduce metabolic activity of cells in biofilms by an average of 70%. Further investigation of biofilm viability by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) demonstrated that extended ACP treatment had a detrimental effect on the viability of P. aeruginosa through disintegration of both bacterial cells and the biofilm matrix. The results of this study demonstrate the potential of a novel, in-package, high-voltage ACP decontamination approach for the inactivation of bacterial biofilms.

show abstract

Section: Discussionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

View full text Add to dashboard Cite

show abstract

“…ACP treated or untreated samples were prepared as described by Srey et al (2014) with minor modifications. The cells were fixed in ice-cold 2.5% glutaraldehyde in 0.05 M sodium cacodylate buffer (pH7.4) (SCB) for 2 h. The cells were washed with the same buffer three times and fixed in 1% osmium tetroxide for 2 h at 4°C.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

“…Moreover, biofilms exhibit increased tolerance to antimicrobial agents. In the United States approximately 80% of persistent bacterial infections were found to be associated with biofilms (Srey et al, 2014). Another factor that could have a potential contribution to elevated resistance to antimicrobial agents is that bacterial pathogens can become internalized inside plant tissue.…”

mentioning

confidence: 99%

Cold plasma inactivation of internalised bacteria and biofilms for Salmonella enterica serovar Typhimurium, Listeria monocytogenes and Escherichia coli

Ziuzina¹,

Han²,

Cullen

et al. 2015

International Journal of Food Microbiology

162

View full text Add to dashboard Cite

Microbial biofilms and bacteria internalised in produce tissue may reduce the effectiveness of decontamination methods. In this study, the inactivation efficacy of in-package atmospheric cold plasma (ACP) afterglow was investigated against Salmonella Typhimurium, Listeria monocytogenes and Escherichia coli in the forms of planktonic cultures, biofilms formed on lettuce and associated bacteria internalised in lettuce tissue. Prepared lettuce broth (3%) was inoculated with bacteria resulting in a final concentration of ~7.0 log 10 CFU/ml. For biofilm formation and internalisation, lettuce pieces (5 x 5 cm) were dip-inoculated in bacterial suspension of ~7.0 log 10 CFU/ml for 2 h and further incubated for 0, 24 and 48 h at either 4°C or room temperature (~22°C) in combination with light/dark photoperiod or at 4°C under dark conditions. Inoculated samples were sealed inside a rigid polypropylene container and However, the existence of biofilms and internalised bacteria should be considered for further optimisation of ACP treatment parameters in order to achieve an effective control of the realistic challenges posed by foodborne pathogens.

show abstract

“…A variety of ready-to-eat food products including fish, meat, poultry, and dairy have been associated with outbreaks (Knabel 2015;EFSA 2015). Listeria monocytogenes is capable of forming matured biofilm on food within 24 h (Srey et al 2014), persisting on food processing equipments and/or slicing materials from which post-cooking contamination of ready-to-eat meat may occur. Moreover, this Grampositive pathogen is capable of growing at refrigeration temperatures which is a major concern in refrigerated foods.…”

Section: Introductionmentioning

confidence: 99%

Use of Rhodomyrtus tomentosa ethanolic leaf extract for the bio-control of Listeria monocytogenes post-cooking contamination in cooked chicken meat

2016

View full text Add to dashboard Cite

Controlling foodborne pathogen in ready-to-eat food is important in food safety. The present study accessed the potential use of Rhodomyrtus tomentosa ethanolic leaf extract as a bio-control agent against Listeria monocytogenes in cooked chicken meat model system. The antilisterial activity of the plant extract was better under microwave condition and enhanced as storage temperature increased from 4 to 37°C. The extract could reduce L. monocytogenes numbers at low (10 4 CFU/g) and high (10 6 CFU/g) inoculum levels in cooked chicken by both rinse and injection application methods. A 5 min rinse in 8% w/v R. tomentosa extract reduced the bacterial number by C2-log before storage and C3-log after storage at 4°C for 5 days. Injection with 0.4% w/w R. tomentosa extract resulted in approximately 2-log reduction in the cell numbers both before and after storage at 4°C for 5 days. Five minutes rinse in the extract bath demonstrated better sensory preferences which were not significantly different from the control. Addition of black pepper powder to the extract rinsed samples improved odour but not appearance, colour, and texture preferences. Rhodomyrtus tomentosa extract was significantly effective for the bio-control of L. monocytogenes contaminations in cooked chicken meat model. The extract was observed as a potent bio-additive agent to control contaminations from L. monocytogenes and ensure safety in ready-to-eat meat.

show abstract

Reduction effect of the selected chemical and physical treatments to reduce L. monocytogenes biofilms formed on lettuce and cabbage

Cited by 61 publications

References 59 publications

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Cold plasma inactivation of internalised bacteria and biofilms for Salmonella enterica serovar Typhimurium, Listeria monocytogenes and Escherichia coli

Use of Rhodomyrtus tomentosa ethanolic leaf extract for the bio-control of Listeria monocytogenes post-cooking contamination in cooked chicken meat

Contact Info

Product

Resources

About