Reduction in Enteroccocus faecalis counts produced by three file systems in severely curved canals

Abstract

Aim: This study evaluated the effectiveness of the mechanical reduction of intracanal bacteria produced by the endodontic systems Reciproc Blue (VDW GmbH), XP-Endo Shaper (FKG Dentaire) and ProTaper Next (Dentsply Sirona Endodontics) in severely curved canals by culture analysis. Methodology: Fifty severely curved mesiobuccal canals of mandibular molars were selected and instrumented. Following this, six specimens were selected as control, while 44 canals were contaminated with Enterococcus faecalis strains (ATCC 29212). Specimens were incubated for 21 days at 37 ºC. Then, contaminated specimens were randomly divided into 3 groups (n=14): ProTaper Next (G1), XP-Endo Shaper (G2) and Reciproc Blue (G3). Control Group: Six non-contaminated canals were prepared with one of the 3 file systems (n=2). Microbial samples were obtained before (S1) and after root canal preparation (S2). Two roots were observed using scanning electron microscopy to verify biofilm formation. Quantitative culture analyses of intracanal E. faecalis reduction was performed using CFUs, and the Wilcoxon test was used for intragroup analysis, and intergroup analysis was performed using Kruskal-Wallis test. The level of significance for all analyses was set at P < .05. Results: All systems presented effective bacterial reduction (p<0.05), but still had bacterial growth. ProTaper Next displayed the highest E. faecalis reduction (P<0.05), while XP-Endo Shaper and Reciproc Blue systems presented similar results (P>0.05). ProTaper Next showed the greatest antibacterial action when compared to the XP-Endo Shaper and Reciproc Blue systems. Conclusion: No instrumentation system rendered root canals completely free from bacteria.

Impact of Different Access Cavity Designs and Ni–Ti Files on the Elimination of Enterococcus faecalis from the Root Canal System: An In Vitro Study

“…Otero et al [30] also found that ProTaper Gold showed superior bacterial reduction compared to WaveOne Gold. Moreover, Nascimento et al [10] reported that the Reciproc Blue file system exhibited lower antibacterial action when compared to the ProTaper Next file system. These results can be attributed to the fact that the ProTaper file system removes the dentin surface more aggressively therefore leading to lower bacterial counts in samples after instrumentation [8].…”

“…Various improved nickel-titanium instrumentation systems with different characteristics have been developed to improve the procedure of intracanal bacterial reduction [9,10]. The ProTaper Gold (Dentsply Maillefer, Ballaigues, Switzerland) file system is produced using M-Wire technology and employs a continuous rotational movement.…”

Eficácia das limas reciprocantes Reciproc Blue e ProDesign R na remoção de material obturador: Uma revisão integrativa da literatura

“…O principal objetivo da terapia endodôntica é o completo selamento do sistema de canais radiculares, para prevenir e tratar a periodontite apical. Muitos fatores têm sido relacionados com o insucesso neste processo, dos quais podemos realçar a permanência da infecção microbiana dentro dos canais radiculares, a complexidade anatômica, irregularidades na dentina, instrumentação e obturação inadequadas (Roças et al, 2016;Nascimento et al, 2021).…”