Objective: This in vitro study was designed to compare the disinfection potential of an Er:YAG photon-initiated photoacousitc (PIPS) irrigation mechanism to standard needle irrigation (SNI) in eliminating biofilms from complex root canal systems.
Method and materials:Twenty-four extracted mandibular molar teeth exhibiting a Vertucci Type II canal system were selected and underwent canal instrumentation to create either a minimal (20/.04) or fully-flared (25/.06) preparation. Teeth were inoculated with bacterial plaque and incubated for 3 weeks creating a mature biofilm. Both instrumentation groups were sub-divided according to mode of canal disinfection: sterile saline or sodium hypochlorite (NaOCl) with PIPS laser-activation or same irrigants delivered with SNI. Each irrigation protocol used similar quantities of NaOCl or saline over the same time periods. Viable intracanal bacteria were quantified by MTT bacterial viability assay. Sub-groups were analyzed for statistical significance by ANOVA, followed by Scheffe's F-test (P = .05).Results: All saline groups showed significantly more viable bacteria after irrigation than the corresponding NaOCl sub-groups. The #20/.04 sub-group that underwent PIPS and NaOCl irrigation had greater disinfection than the SNI counterpart, which was not significant (P > 0.05). The #25/.06 PIPS/NaOCl subgroup showed greater disinfection than SNI (P > 0.0008). All other groups showed no significant differences in disinfection of root canals.
Conclusion:In this in vitro study PIPS laser-activated irrigation achieved higher disinfection in #25/.06 fully-flared preparations than SNI. Although not significantly different, greater disinfection was found in the #20/.04 minimal canal preparations that were treated with PIPS irrigation compared to SNI.
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In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars 2/5Copyright: ©2017 Garcia-Godoy et al. [19,20]. The combination of varying tip size and taper continues to be studied, aimed at improving canal disinfection while conserving dentin. As apical preparation size increases, irrigant replacement was enhanced, providing space existed between the needle and the canal wall [21]. Increased canal taper also improved fluid interchange and reduced the risk of extrusion; minimal taper with large apical preparations also improved irrigant replacement [22]. Ex vivo investigation of the optimal tip size taper combination for laser activated irrigation have yet to be determined. The objective of this study was to examine the disinfection of intentionally contaminated Type II canals in the mesial root of mandibular molars by comparing needle flushing versus PIPS activation during endodontic irrigation of complex Vertucci Type II canal systems of two different sizes and taper. The null hypothesis was that no difference exists between bacterial disinfection present in #20/.04 taper and #25/.06 flared taper preparations using either PIPS or needle irrigation.
Methods and MaterialsToot...