Reduction of 3-chlorobenzoate, 3-bromobenzoate, and benzoate to corresponding alcohols by Desulfomicrobium escambiense, isolated from a 3-chlorobenzoate-dechlorinating coculture

Genthner, Barbara R. Sharak; Townsend, G. Todd; Blattmann, Beat O.

doi:10.1128/aem.63.12.4698-4703.1997

Cited by 22 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the roles of other abundant organisms such as Desulfomicrobium, Desulfovibrio, Clostridium, and Pseudomonas in the transformation of TCEP and/or TCPP cannot be excluded, although their relative abundances showed a trend similar to those in the treatment controls without the addition of TCEP/TCPP. Previous studies reported that Desulfomicrobium, Desulfovibrio, and Clostridium are capable of reductively dehalogenating halogenated substances. − Pseudomonas, the most abundant genus in the culture 8P, has been reported to be able to catalyze the hydrolysis of organophosphates . It is thus possible that Pseudomonas may contribute to the phosphoester bond hydrolysis of TCPP.…”

Section: Resultsmentioning

confidence: 99%

Dehalococcoides-Containing Enrichment Cultures Transform Two Chlorinated Organophosphate Esters

Zhu

Deng

Fang

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

Although chlorinated organophosphate esters (Cl-OPEs) have been reported to be ubiquitously distributed in various anoxic environments, little information is available on their fate under anoxic conditions. In this study, we report two Dehalococcoides-containing enrichment cultures that transformed 3.88 ± 0.22 μmol tris(2-chloroethyl) phosphate (TCEP) and 2.61 ± 0.02 μmol tris(1-chloro-2-propyl) phosphate (TCPP) within 10 days. Based on the identification of the transformed products and deuteration experiments, we inferred that TCEP may be transformed to generate bis(2-chloroethyl) phosphate and ethene via one-electron transfer (radical mechanism), followed by C−O bond cleavage. Ethene was subsequently reduced to ethane. Similarly, TCPP was transformed to form bis(1-chloro-2-propyl) phosphate and propene. 16S rRNA gene amplicon sequencing and quantitative polymerase chain reaction analysis revealed that Dehalococcoides was the predominant contributor to the transformation of TCEP and TCPP. Two draft genomes of Dehalococcoides assembled from the metagenomes of the TCEP-and TCPP-transforming enrichment cultures contained 14 and 15 putative reductive dehalogenase (rdh) genes, respectively. Most of these rdh genes were actively transcribed, suggesting that they might contribute to the transformation of TCEP and TCPP. Taken together, this study provides insights into the role of Dehalococcoides during the transformation of representative Cl-OPEs.

show abstract

Section: Resultsmentioning

confidence: 99%

Dehalococcoides-Containing Enrichment Cultures Transform Two Chlorinated Organophosphate Esters

Zhu

Deng

Fang

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…From the relative abundance of the two genera Geobacter and Pseudomonas, reactor B (11.0 %) was significantly higher than reactor A (3.6 %). However, the majority of the bacteria in the anode of the reactor A were gram-negative bacteria, such as Sulfuritalea [49,68], Aquaspirillum, Arcobacter, Desulfomicrobium [69], Pseudomonas [70], Acidaminobacter [71], Geobacter. Reactor B contained a number of gram-positive bacteria, such as Anaerofustis [66], Sedimentibacter [72], and Desulfitobacterium [73].…”

Section: Discussionmentioning

confidence: 99%

Factors Affecting Wastewater Treatment and Power Generation of Constructed Wetland Microbial Fuel Cell Systems

Wan¹,

Ren²,

Wang³

et al. 2021

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

Although there have been many studies on the process conditions of Constructed Wetlands (CW) and Microbial Fuel Cells (MFC), there are few studies on the Constructed Wetlands coupled with Microbial Fuel Cells. Currently, low power production is the main problem faced by Constructed Wetlands-Microbial Fuel Cell systems (CW-MFCs). This experiment intends to research the effects of HRT, influent COD and electrode spacing on wastewater treatment and power generation performance. In this experiment, CW-MFCs with two different electrode spacings (18 cm for reactor A and 28 cm for reactor B) were set up under continuous flow conditions. The effects of HRT, influent COD and eletrode spacing on wastewater treatment and power generation performance were explored using a single-factor controlled variable method. Experiment results showed that the extension of HRT was beneficial for contaminants removal and the power generation of the CW-MFCs, but the excessive extension was ineffective for the electricity output. The optimal HRT of the CW-MFCs was 24 h when influent COD was 500 mg•l -1 . Improving influent COD within the appropriate range (500~1000 mg•l -1 ) facilitated the power generation and contaminant removal performance of the CW-MFCs. The maximum output voltage and power density were obtained in reactor A when influent COD was 1000 mg•l -1 and HRT was 24 h, which were 548 mV and 120 mW•m -3 , respectively. Compared with reactor B, reactor with smaller electrode spacing achieved better electricity generation and contaminant degradation under the optimal condition. Its average output voltage could be improved by 5.1~46.1% and the removal rates of COD and NH 4 + -N could also be improved by 0.2~4.9 % and -0.9~13.7 %, respectively. This phenomenon indicates that there was a significant positive correlation between the number of Gram-negative bacteria and

show abstract

“…The final degradation products of aerobic PCB-degrading bacteria are chlorobenzoic acids (Sondossi et al, 1992). For further degradation leading to mineralization, halobenzoate-degrading bacterial populations, which are commonly found in sediments (Van der Woude et al, 1996;Genthner et al, 1997;Townsend et al, 19971, are needed.…”

mentioning

confidence: 99%

Microbial Degradation of PCBs in Contaminated Sediments

Rhee

Cho

Ostrofsky

1999

Remediation Journal

View full text Add to dashboard Cite

Reduction of 3-chlorobenzoate, 3-bromobenzoate, and benzoate to corresponding alcohols by Desulfomicrobium escambiense, isolated from a 3-chlorobenzoate-dechlorinating coculture

Cited by 22 publications

References 34 publications

Dehalococcoides-Containing Enrichment Cultures Transform Two Chlorinated Organophosphate Esters

Dehalococcoides-Containing Enrichment Cultures Transform Two Chlorinated Organophosphate Esters

Factors Affecting Wastewater Treatment and Power Generation of Constructed Wetland Microbial Fuel Cell Systems

Microbial Degradation of PCBs in Contaminated Sediments

Contact Info

Product

Resources

About