Reduction of Adrenal-Steroid Production by an Inhibitor of Cholesterol Biosynthesis

Jc, Melby; M, St Cyr; Sl, Dale

doi:10.1056/nejm196103232641202

Cited by 35 publications

(7 citation statements)

References 9 publications

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“…The present study also indicates that triparanol has little or no effect upon the aldosterone secretion rate or upon the renal excretion of sodium even though the drug has been found to decrease the excretion of the "tetrahydro" metabolite of aldosterone (10).…”

Section: Discussionsupporting

confidence: 48%

“…It has been shown previously that triparanol, an inhibitor of cholesterol biosynthesis, decreases urinary 17-OHCS in man (10). The mechanism through which the decrease in urinary 17-OHCS was brought about was not elucidated, although it was postulated that triparanol caused a decrease in the secretion of adrenal corticosteroids.…”

Section: Discussionmentioning

confidence: 99%

“…In rats, triparanol does not impair the adrenal secretion of corticosterone in response to ACTH. urinary 17-hydroxycorticosteroids (17-OHCS) of human subjects fall during the administration of large doses of triparanol (10).…”

mentioning

confidence: 99%

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Effects of Triparanol on the Secretion and Metabolism of Adrenal Corticosteroids

Ney¹,

Coppage²,

Shimizu³

et al. 1962

The Journal of Clinical Endocrinology & Metabolism

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

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Effects of Triparanol on the Secretion and Metabolism of Adrenal Corticosteroids

Ney¹,

Coppage²,

Shimizu³

et al. 1962

The Journal of Clinical Endocrinology & Metabolism

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“…Für das Tripanarol sind Hautveränderungen in Form von Ichthyosis und Alopezie beschrieben worden [5]. W eiterhin scheint es die Produktion der Nebennierenrindenhormone zu beeinflussen [6]. Hohe Dosen von Niko tinsäure können gleichfalls Hauterscheinungen erzeugen sowie gastro intestinale Störungen und Hyperurikäm ie [7].…”

Section: Laubingerunclassified

Cholesterinsenkende Diät

Laubinger¹

1962

Digestion

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“…From our attempts to obtain samples, we found that the only accessible anti-inflammatory steroids were : betamethasone, dexamethasone, fludrocortisone, 6a-methylhydrocortisone, 6a-methylprednisolone, prednisolone, prednisone and triamcinolone. These were used as the alcohols since their 21-esters would be hydrolysed to the parent alcohols in the body (Voigt, 1959;Melby & St. Cyr, 1961). These steroids, and hydrocortisone and cortisone, and their separation, form the subject of this paper.…”

mentioning

confidence: 99%

The detection and identification of synthetic steroids in horse urine

Moss

Rylance

1966

Journal of Pharmacy and Pharmacology

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A scheme for the detection of synthetic steroids which could be used in the "doping" of race-horses is described. The method involves extraction from urine into ethyl acetate : ether (1 : 1) followed by initial two-dimensional thin-layer chromatography using (1) ethyl acetate (2) methylene chloride : dioxan :water (100: 50 : 50). Elution of the ultraviolet absorbing spots for extinction measurements is followed by further one way thin-layer chromatography as free alcohols in amyl acetate : acetone (1 : 1). Further chromatography of the steroid alcohols in chloroform : ether : water (80 : 20 : 0.5) on formamide-impregnated plates and chromatography of the acetates in ether helps to identify the unknown steroid. Additional identification is made by colour reactions with (1) a tetrazolium reagent (2) vanillin : perchloric acid. NTI-INFLAMMATORY steroids are now widely used both inA veterinary and human medicine. Their possible use to influence the performance of racehorses caused us to investigate methods for their detection. We report a method for the detection of the steroids most likely to be used in this way. The method enables a separation to be made between any of these synthetic steroids and the naturally occurring steroids in horse urine. For the purpose of identifying the presence of "dope", we developed a method which would detect unchanged drug because (i) this was easier than looking for conjugated forms of drug which would need to be hydrolysed ; (ii) it made the diagnosis of "doping" more certain in cases where metabolites might be the same as those from endogenous steroids, e.g. metabolites from prednisolone and hydrocortisone ; (iii) detailed metabolism studies were not necessary (although work on this aspect has already commenced). For speed, we considered a method based on thin-layer chromatography would be preferable to any based on paper chromatography. From our attempts to obtain samples, we found that the only accessible anti-inflammatory steroids were : betamethasone, dexamethasone, fludrocortisone, 6a-methylhydrocortisone, 6a-methylprednisolone, prednisolone, prednisone and triamcinolone. These were used as the alcohols since their 21-esters would be hydrolysed to the parent alcohols in the body (Voigt, 1959;Melby & St. Cyr, 1961). These steroids, and hydrocortisone and cortisone, and their separation, form the subject of this paper. Experiment a1 MATERIALS

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Reduction of Adrenal-Steroid Production by an Inhibitor of Cholesterol Biosynthesis

Cited by 35 publications

References 9 publications

Effects of Triparanol on the Secretion and Metabolism of Adrenal Corticosteroids

Effects of Triparanol on the Secretion and Metabolism of Adrenal Corticosteroids

Cholesterinsenkende Diät

The detection and identification of synthetic steroids in horse urine

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