Reduction of diabetes-induced oxidative stress by phosphodiesterase inhibitors in rats

Milani, Elham A.; Nikfar, Shekoufeh; Khorasani, Reza; Zamani, Mohammad Jafar; Abdollahi, Mohammad

doi:10.1016/j.cca.2005.02.010

Cited by 117 publications

(79 citation statements)

References 44 publications

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“…A number of evidence on the relationship between oxidative stress and diabetes (32)(33)(34)(42)(43)(44)(45)(46)(47) even by OP compounds (48) supports this conclusion, and calls for further research of the benefi cial effects of antioxidants (49). Our future work will focus on the balance between insulin secretion, antioxidant levels, and islet cytotoxicity.…”

Section: Figure 6 Insulin Release From Islets Of Langerhans Incubatedmentioning

confidence: 76%

Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers On Diazinon-Induced Hyposecretion Of Insulin From Isolated Islets of Langerhans in Rats

Pourkhalili

Pournourmohammadi

Rahimi

et al. 2009

Archives of Industrial Hygiene and Toxicology

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Hyperglycaemia has been observed with exposure to organophosphate insecticides. This study was designed to compare the effects of calcium channel blockers, alpha-adrenergic, beta-adrenergic, and muscarinic receptor blockers, and of free radical scavengers on insulin secretion from diazinon-treated islets of Langerhans isolated from the pancreas of rats using standard collagenase digestion, separation by centrifugation, and hand-picking technique. The islets were then cultured in an incubator at 37 °C and 5 % CO 2 . In each experimental set 1 mL of 8 mmol L -1 glucose plus 125 µg mL -1 or 625 µg mL -1 of diazinon were added, except for the control group, which received 8 mmol L -1 glucose alone. The cultures were then treated with one of the following: 30 µmol L -1 atropine, 100 µmol L -1 ACh + 10 µmol L -1 neostigmine, 0.1 µmol L -1 propranolol, 2 µmol L -1 nifedipine, 50 µmol L -1 phenoxybenzamine, or 10 µmol L -1 alphatocopherol. In all experiments, diazinon signifi cantly reduced glucose-stimulated insulin secretion at both doses, showing no dose dependency, as the average inhibition for the lower dose was 62.20 % and for the higher dose 64.38 %. Acetylcholine and alpha-tocopherol restored, whereas atropine potentiated diazinoninduced hyposecretion of insulin. Alpha-, beta-and calcium channel blockers did not change diazinoninduced effects. These fi ndings suggest that diazinon affects insulin secretion mainly by disturbing the balance between free radicals and antioxidants in the islets of Langerhans and by inducing toxic stress.

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Section: Figure 6 Insulin Release From Islets Of Langerhans Incubatedmentioning

confidence: 76%

Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers On Diazinon-Induced Hyposecretion Of Insulin From Isolated Islets of Langerhans in Rats

Pourkhalili

Pournourmohammadi

Rahimi

et al. 2009

Archives of Industrial Hygiene and Toxicology

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“…Oxygen-free radical production related to ischemia/reperfusion after SWL is believed to contribute to parenchymal damage via lipid peroxidation and the disruption of cellular and mitochondrial membranes, and cellular damage (21). There is evidence that biological responses triggered by oxidative products are associated with lipid peroxidation derivatives, which are able to induce various pathogenic intracellular signals involving calcium, G-proteins, cAMP, cGMP, phospholipase C and D, protein kinase C, ceramide, and MAP kinase cascade leading to cellular dysfunction (21).…”

Section: Discussionmentioning

confidence: 99%

“…There is evidence that biological responses triggered by oxidative products are associated with lipid peroxidation derivatives, which are able to induce various pathogenic intracellular signals involving calcium, G-proteins, cAMP, cGMP, phospholipase C and D, protein kinase C, ceramide, and MAP kinase cascade leading to cellular dysfunction (21).…”

Section: Discussionmentioning

confidence: 99%

Reduction of shock wave lithotripsy-induced renal tubular injury by tadalafil

Me¹,

Aytaç²,

Kilicaslan³

et al. 2013

BLL

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Abstract:Objectives: The aim of the study was to examine whether administration of tadalafi l, a phosphodiesterase type 5 inhibitor, has a protective effect in the prevention of renal injury in a rat model after Shock Wave Lithotripsy (SWL), with the assessment based on histopathologic examination and measurement of Heat Shock Protein 70 expression. Methods: A total of 40 adult, male Sprague-Dawley rats were divided into fi ve groups as follows; control group, group SN3, group SN7, group TSN3, TSN7. Both kidneys were evaluated regarding tubular damage, peritubular fi brosis and glomerular damage using light microscopy. We examined HSP-70 expression, which occurred in response to renal ischemic injury observed after SWL. The groups were compared between each other and with the control group. Results: No statistically signifi cant difference was found when the groups were compared using light microscopy for the changes in glomeruli. Tubular necrosis, loss of microvilli and peritubular fi brosis were less in Group TSN3 and Group TSN7 compared to Group SN3. Similarly, tubular necrosis, loss of microvilli and peritubular fi brosis were less in Group TSN3 and Group TSN7 compared to Group SN7. HSP-70 staining was less in Group TSN3 and Group TSN7 compared to Group SN3 and Group SN7. Conclusion: Based on the results of light microscopy and HSP-70 staining, we demonstrated that SWL could cause renal ischemia-reperfusion injury. Our results suggested that Tadalafi l administration could prevent this SWL-related renal cell injury (Tab. 2, Fig. 5, Ref. 28). Text in PDF www.elis.sk.

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“…Of course, the lack of human studies limits our conclusions regarding in-vivo research and necessitates further investigations to establish the benefits of antioxidants in human islet transplantation. Fortunately, there is sufficient evidence on the existence of oxidative stress in diabetes and the significant role of antioxidants in the reduction of diabetic complications [69][70][71][72][73][74][75][76] .…”

Section: Discussionmentioning

confidence: 99%

Islet transplantation and antioxidant management: A comprehensive review

Monfared¹,

Larijani

Abdollahi

2009

WJG

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Islet transplantation as a promising treatment for type 1 diabetes has received widespread attention. Oxidative stress plays an essential role in cell injury during islet isolation and transplantation procedures. Antioxidants have been used in various studies to improve islet transplantation procedures. The present study reviews the role of oxidative stress and the benefits of antioxidants in islet transplantation procedures. The bibliographical databases Pubmed and Scopus were searched up to November 2008.All relevant human and animal in-vivo and in-vitro studies, which investigated antioxidants on islets, were included. Almost all the tested antioxidants used in the in-vitro studies enhanced islet viability and insulin secretion. Better control of blood glucose after transplantation was the major outcome of antioxidant therapy in all in-vivo studies. The data also indicated that antioxidants improved islet transplantation procedures. Although there is still insufficient evidence to draw definitive conclusions about the efficacy of individual supplements, the benefits of antioxidants in islet isolation procedures cannot be ignored.

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Reduction of diabetes-induced oxidative stress by phosphodiesterase inhibitors in rats

Cited by 117 publications

References 44 publications

Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers On Diazinon-Induced Hyposecretion Of Insulin From Isolated Islets of Langerhans in Rats

Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers On Diazinon-Induced Hyposecretion Of Insulin From Isolated Islets of Langerhans in Rats

Reduction of shock wave lithotripsy-induced renal tubular injury by tadalafil

Islet transplantation and antioxidant management: A comprehensive review

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