Reduction of hexavalent chromium by Exiguobacterium mexicanum isolated from chromite mines soil

Das, Sasmita; Behera, Bikash Chandra; Mohapatra, Ranjan Kumar; Pradhan, Biswaranjan; Sudarshan, M.; Chakraborty, Anindita; Thatoi, Hrudayanath

doi:10.1016/j.chemosphere.2021.131135

Cited by 38 publications

(31 citation statements)

References 57 publications

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“…Based on this information, we think that the Cr form accumulated in the cells in our study may be Cr(III). Similar to our ndings, Das et al (2021) suggested that the Cr particles adsorbed or precipitated on the outer surface of the cells from the EDX peaks obtained from the bacterial cells to which Cr(VI) was applied may be the reduced form of Cr(III). On the other hand, Zhu et al (2008) reported that the EDX peaks of the bacterial cells to which they applied Cr(VI) may be due to FTIR analysis results showed that -OH, methyl, sulfate groups of MT2A cells were effective in Cr(VI) binding.…”

Section: Discussionsupporting

confidence: 89%

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Akkurt¹,

Oğuz²,

Uçkun³

2021

Preprint

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Studies on the removal of toxic metals from water by genetic engineering applications are becoming more and more diverse. Especially by the expression of cysteine and thiol rich proteins such as metallothioneins in bacterial cells, a high rate of removal of metal ions from the environment is ensured. In this study, we evaluated the removal and reduction of hexavalent chromium Cr(VI)from aqueous solutions with the recombinant strains obtained by cloning the human metallothioneinsMT2A and MT3 into Escherichia coli Jm109.E. coli MT2A was the most effective strain in both Cr(VI) removal (89%in 25 mg/L Cr(VI) application) and Cr(VI) reduction (76%in 25 mg/L Cr(VI) application).Cr(VI) adsorbed per dry cell (at 25 mg/L) were 17 mg/g, 22 mg/g and 19 mg/g for Jm109, MT2A and MT3, respectively. Scanning electron microscope (SEM) images showed that the morphological structures of Cr(VI)treated cells were significantly damaged compared to control cells. Scanning transmission electron microscope (STEM) images showed black spots in the cytoplasm of cells treated with Cr(VI).From the shifts in the Fourier transform infrared spectroscopy analysis (FTIR) spectra of the cells treated with Cr(VI) compared to the control cells, it was determined that the groups interacting with Cr were amino, hydroxyl, methyl and sulfhydryl. When all these experimental data were evaluated together, it was concluded that all three species were effective in removing Cr(VI) from aqueous solutions and MT2A strain could be used as the most effective biotechnological tool.

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Section: Discussionsupporting

confidence: 89%

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Akkurt¹,

Oğuz²,

Uçkun³

2021

Preprint

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“…In the presence of Pb + , Zn 2+ , Co 2+ , Mn 2+ , Ni 2+ and Mo 2+ , the Cr(VI) bioreduction was slightly affected by about 10-15% of the control (no metal). However, in comparison to the control, Cd 2+ caused about 50% decrease of Cr(VI) bioreduction, which is in agreement with a previous report by Das et al (2021) for Cr(VI) reduction by Exiguobacterium mexicanum. The decrease in Cr(VI) reduction by Cd may be due to either higher toxicity of the metal or inhibition of chromate reductase enzyme (Megharaj et al 2003).…”

Section: Effect Of Co-existing Ionssupporting

confidence: 92%

“…However, by increasing the aeration level (200 rpm), the Cr(VI) reduction significantly decreased. Higher shaking speed can increase the oxygen pressure and reduce the partial pressure of the dissolved CO 2 in the liquid medium, hence affecting the bacterial metabolism (Das et al 2021). A similar effect of the varied shaking speed on Cr(VI) bioreduction has been also reported for other bacteria (Mishra et al 2012;Das et al 2014Das et al , 2021Tan et al 2020).…”

Section: Effect Of Aeration Levelsupporting

confidence: 60%

“…The pH of the culture medium can affect the efficiency of bacterial removal of heavy metals by affecting the enzymatic activity, metal binding to the cell and the availability of metal ions as well as the bacterial growth (Wu et al 2019;Das et al 2021). The effect of the medium pH on Cr(VI) bioreduction by S. agaradhaerens NRC-R is shown in Fig.…”

Section: Effect Of Phmentioning

confidence: 99%

“…Higher shaking speed can increase the oxygen pressure and reduce the partial pressure of the dissolved CO 2 in the liquid medium, hence affecting the bacterial metabolism (Das et al 2021). A similar effect of the varied shaking speed on Cr(VI) bioreduction has been also reported for other bacteria (Mishra et al 2012;Das et al 2014Das et al , 2021Tan et al 2020). Generally, Cr(VI) bioreduction takes place in the presence of oxygen in three steps, including reduction of Cr(VI) to Cr(V) and/or Cr(IV) intermediates, followed by a further reduction to the thermodynamically stable Cr(III) (Cheung and Gu 2007).…”

Section: Effect Of Aeration Levelmentioning

confidence: 99%

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Bioreduction of hexavalent chromium by a novel haloalkaliphilic Salipaludibacillus agaradhaerens strain NRC-R isolated from hypersaline soda lakes

et al. 2021

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A novel Cr(VI)-resistant haloalkaliphilic bacterial strain NRC-R, identified as Salipaludibacillus agaradhaerens, was isolated from hypersaline soda lakes and characterized for its Cr(VI) bioreduction efficiency. Strain NRC-R grew well and effectively reduced Cr(VI) under a wide range of sodium chloride, pH, shaking velocity and temperature, showing maximum Cr(VI) reduction at 8% NaCl, pH 10, 150 rpm and 35 °C, respectively. Strain NRC-R was able to grow and reduce Cr(VI) effectively in the presence of different heavy metals and oxyanions (Pb 2+ , Zn 2+ , Co 2+ , Mn 2+ , Ni 2+ , Mo 2+ , HPO 4 − , NO 3 − , SO 4 2− and HCO 3 − ). Furthermore, Fe 3+ and Cu 2+ significantly enhanced the Cr(VI) removal by about 1.5 fold. Strain NRC-R could reduce Cr(VI) using a variety of electron donors, exhibiting a maximum reduction in the presence of NADH and fructose. The bioremoval of Cr(VI) using strain NRC-R was due to direct enzymatic reduction and the chromate reductase activity was mainly detected in the bacterial cell membrane. Under the optimized conditions, strain NRC-R showed a remarkable Cr(VI) bioreduction with highest reduction rate of 240 uM/h. Cr(VI) concentrations of up to 3 mM (888.5 mg/L) and 4 mM (1177 mg/L) were completely reduced within 16 h and 32 h, respectively. TEM and SEM-EDX analyses confirmed the biosorption of chromium species into the cells. To the best of our knowledge, this is the first report about Cr(VI) reduction by S. agaradhaerens. In conclusion, S. agaradhaerens NRC-R was a highly efficient Cr(VI) reducing haloalkaliphilic bacterium that has a significant potential in the bioremediation of Cr(VI)-contaminated environments.

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Employing Microbes for Cr Alleviation: A Reliant Harmless Approach

Prabhu,

Jayakumar,

Gomadurai

et al. 2023

Environmental Science and Engineering

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Reduction of hexavalent chromium by Exiguobacterium mexicanum isolated from chromite mines soil

Cited by 38 publications

References 57 publications

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Bioreduction and Bioremoval of Hexavalent Chromium by Genetically Engineered Strains (Escherichia Coli MT2A and Escherichia Coli MT3)

Bioreduction of hexavalent chromium by a novel haloalkaliphilic Salipaludibacillus agaradhaerens strain NRC-R isolated from hypersaline soda lakes

Employing Microbes for Cr Alleviation: A Reliant Harmless Approach

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