Reduction of oxidative stress of normal and G6PD enzyme-deficient human erythrocytes by Centaurea ammocyanus extract (in vitro study)

Joujeh, Ruba; Zaid, Salim; Mona, S. R.

doi:10.29090/psa.2020.02.019.0003

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…However, Centaurea ammocyanus has a little amount of saponins 45 . Achillea santolina contains phenolic and flavonoids compounds, and this matches what Al-Snafi has reported in his results 46 . Tannins, steroids, and terpenoids were found in Achillea tenuifolia LAm extract 47 .…”

Section: Phytochemical Screeningsupporting

confidence: 91%

Phytochemical Study and Protective Effect Investigation Against Oxidative Damage in Human Erythrocytes of Syrian Asteraceae Plants

Aljaja

Lahdo

Kitaz

2021

Bulletin of Pharmaceutical Sciences. Assiut

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Oxidative stress is a major cause of many diseases, it can damage the tissues and lead to lipid peroxidation and DNA strand breakage. Nowadays, researchers have centered medicinal plants in their concern, to benefit from their active component as antioxidant reagents. This study aims to investigate the protective and hemolytic activity, as well as qualitative and quantitative chemical content of plants from Syrian Flora. Ethanol 70% extracts of three plants of Asteraceae; Onopordum carduiforme, Centaurea verutum, and Achillea santolina were prepared to test their protective antioxidant effect on erythrocytes membrane against the oxidative hemolysis which was induced using AAPH. The hemolytic effect of these plants was also evaluated. In addition to the quantitative content of phenols TPC and flavonoids TFC compounds using Folin-Ciocalteu and aluminum chloride colorimetric methods respectively. The basic phytochemical screening was conducted using the standard methods.The results showed a low hemolytic effect of the extracts on erythrocytes, using the concentrations of (1-5 mg/ml). Moreover, they reduced membrane lipid peroxidation significantly; where O. carduiforme showed the highest efficacy of protective activity against hemolytic damage. The phytochemical screening of the plants' extracts showed phenols, flavonoids, tannins, coumarins, cardiac glycosides, terpenoids, steroids, and carbohydrates presence, whereas no alkaloids or saponins were found. TPC and TFC content ranges were between (9.22 ± 0.412 and 13.716 ± 0.431 mg GAE/g DW) and (2.224 ± 0.346 and 8.958 ± 0.216 mg RUE/g DW) respectively.

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Section: Phytochemical Screeningsupporting

confidence: 91%

Phytochemical Study and Protective Effect Investigation Against Oxidative Damage in Human Erythrocytes of Syrian Asteraceae Plants

Aljaja

Lahdo

Kitaz

2021

Bulletin of Pharmaceutical Sciences. Assiut

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“…Phenolic compounds have been demonstrated to scavenge and react with free radicals, effectively ending the free radical reaction chain. As a result, reducing the oxidative modification of erythrocyte lipids using antioxidants is the recommended method of preventing hemolysis (22) . Several researchers claim that membranous phospholipids have the capacity to bind to flavonoids and other polyphenols, preventing the degradation of membranes brought on by fatty acids (23) .…”

Section: Ex Vivo Antioxidant Assaymentioning

confidence: 99%

Synergistic Antioxidative Potential of Cosmosiin and Vanillic Acid in vitro and ex vivo

Lalfakawmi,

Lalsangpuii,

Tochhawng

et al. 2023

IJST

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Objectives:To determine the synergistic antioxidative potential of cosmosiin and vanillic acid. Methods: The synergistic antioxidative potential of cosmosiin and vanillic acid was evaluated using their ability to scavenge 2, 2 ′azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide anions (O 2• − ), and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). The ability to convert Fe 3+into Fe 2+ was used to evaluate the combined reducing power of cosmosiin and vanillic acid. The synergistic effect of cosmosiin and vanillic acid was also tested in an ex vivo setting using mouse erythrocytes and livers, respectively, for their anti-haemolytic activity and inhibitory effects on lipid peroxidation. Findings: When compared to the individual compound, the mixture of cosmosiin and vanillic acid exhibited higher scavenging activities for DPPH, ABTS and O 2 • − with IC 50 of 112.0 ± 0.46 µM, 126.17 ± 4.97 µM, and 5.14 ± 0.18 µM, respectively. Consistently, the combination of cosmosiin and vanillic acid possessed higher reducing power than either compound alone. A mixture of cosmosiin and vanillic acid also showed higher inhibitory activities against mice erythrocyte hemolysis and lipid peroxidation in the liver homogenate with an inhibition rate of 51 % and 90% respectively. Novelty: Our study corroborates that cosmosiin and vanillic acid have stronger antioxidative effects when used in combination than when used individually.

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Reduction of oxidative stress of normal and G6PD enzyme-deficient human erythrocytes by Centaurea ammocyanus extract (in vitro study)

Cited by 2 publications

References 10 publications

Phytochemical Study and Protective Effect Investigation Against Oxidative Damage in Human Erythrocytes of Syrian Asteraceae Plants

Phytochemical Study and Protective Effect Investigation Against Oxidative Damage in Human Erythrocytes of Syrian Asteraceae Plants

Synergistic Antioxidative Potential of Cosmosiin and Vanillic Acid in vitro and ex vivo

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