Reduction of the Intestinal Absorption of Glucose, Methionine and Sodium Butyrate by the Cation Trimethylhexadecylammonium

Nissim, J. A.

doi:10.1038/187308a0

Cited by 28 publications

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“…In principle, the apparatus is similar to that designed by Sheff & Smyth (1955) for the study of in vivo intestinal absorption in the rat, and used in previous experiments by one of us (Nissim, 1960c). The fluid is circulated by a stream of moist air or oxygen in both cases.…”

Section: Discussionmentioning

confidence: 99%

“…An absorption percentage is taken as the fraction of the nutrient absorbed out of the total originally used in the experiment, expiessed as a percentage. It was therefore necessary as a first step to find the shortest time and the smallest quantities of fluid and nutrients which would give such a suitable absorption percentage.The author has already shown that cetrimonium inhibits the absorption of glucose in the rat and rabbit, and of sodium butyrate and methionine in the rat (Nissim, 1960c). The inhibitory effect of phloridzin on glucose and on butyrate absorption is well known (Jervis et al, 1956;Davson, 1959), but their precise relative activities have not been determined.…”

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“…The author has already shown that cetrimonium inhibits the absorption of glucose in the rat and rabbit, and of sodium butyrate and methionine in the rat (Nissim, 1960c). The inhibitory effect of phloridzin on glucose and on butyrate absorption is well known (Jervis et al, 1956;Davson, 1959), but their precise relative activities have not been determined.…”

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confidence: 99%

“…Both methods were improved by Smyth and co-workers (Jervis, Johnson, Sheff & Smyth, 1956;Smyth & Taylor, 1957). The intestinal perfusion method was used in the anaesthetized rat and rabbit to study the effect of cetrimonium on the absorption of three substances, namely glucose, sodium butyrate and methionine, in a single experiment (Nissim, 1960c). In the present work, the aim was to devise a method for the rapid assay of substances which inhibit or stimulate absorption, so that the percentage absorption of the nutrients studied had to be about 50% in control experiments.…”

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confidence: 99%

“…Residual fluid in the reservoir and tubing was sucked with a pipette and the total volume measured. The net amount of fluid absorbed from the lumen or secreted into it per hour was calculated, and the percentage absorptions of glucose, sodium butyrate and methionine were obtained by the methods described previously (Nissim, 1960c).…”

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The Study and Assay of Substances Affecting Intestinal Absorption in the Mouse

Nissim

1965

British Journal of Pharmacology and Chemotherapy

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Knowledge of how substances are absorbed by the intestine is scanty (Davson, 1959). The discovery of the inhibitory effect of the cation hexadecyltrimethylammonium (cetrimonium) on intestinal absorption provides a new tool for further investigations (Nissim, 1960a,b,c). Precise and rapid methods of assay are needed in order to study the different problems of intestinal absorption as well as to test substances influencing this function. Of substances related to cetrimonium alone, a large number is available (Lawrence, 1950).Methods hitherto used in the investigation of intestinal absorption include the intraluminal perfusion of the rat intestine either as an isolated preparation or in the anaesthetized animal. Both methods were improved by Smyth and co-workers (Jervis, Johnson, Sheff & Smyth, 1956;Smyth & Taylor, 1957). The intestinal perfusion method was used in the anaesthetized rat and rabbit to study the effect of cetrimonium on the absorption of three substances, namely glucose, sodium butyrate and methionine, in a single experiment (Nissim, 1960c). In the present work, the aim was to devise a method for the rapid assay of substances which inhibit or stimulate absorption, so that the percentage absorption of the nutrients studied had to be about 50% in control experiments. An absorption percentage is taken as the fraction of the nutrient absorbed out of the total originally used in the experiment, expiessed as a percentage. It was therefore necessary as a first step to find the shortest time and the smallest quantities of fluid and nutrients which would give such a suitable absorption percentage.The author has already shown that cetrimonium inhibits the absorption of glucose in the rat and rabbit, and of sodium butyrate and methionine in the rat (Nissim, 1960c). The inhibitory effect of phloridzin on glucose and on butyrate absorption is well known (Jervis et al., 1956;Davson, 1959), but their precise relative activities have not been determined. These two substances were therefore selected for comparative study and assay by the new method. METHODSThe mice were pure C3H or CBA strain, 6 to 12 months old. The two types of perfusion apparatus used are described in the appendix. The control fluid consisted of saline (0.9% w/v) containing glucose, sodium butyrate and methionine in concentrations of 0.2% (w/v), these representing, respectively, carbohydrates, short-chain fatty acids and amino acids. Cetrimonium bromide and phloridzin (obtained from I.C.I. and Light & Co., respectively) were added to give concentrations of 10-6 to 10-'. All the solutions were approximately neutral.

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Section: Discussionmentioning

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