Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow

Polini, Alessandro; Mele, Elisa; Sciancalepore, Anna Giovanna; Girardo, Salvatore; Biasco, Adriana; Camposeo, Andrea; Cingolani, R.; Weitz, David A.; Pisignano, Dario

doi:10.1063/1.3481776

Cited by 24 publications

(13 citation statements)

References 43 publications

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“…The heaters used for the oscillatoryflow PCR have changed from the metal/ceramic blocks with a certain heating cartridge (Chiou et al 2001(Chiou et al , 2002Auroux et al 2003a;Hardt et al 2004;Münchow et al 2005;Frey et al 2007;Sugumar et al 2010) to the Peltier heater (Chen et al 2007b;Paik et al 2007) and the film heaters integrated on the chips (for example indium tin oxide (ITO) (Cheng et al 2005), platinum (Bu et al 2003;Wang et al 2005;Polini et al 2010;Sciancalepore et al 2011), chromium/aurum (Ugsornrat et al 2008), and others (Baker et al 2003;Auroux et al 2003bAuroux et al , 2004aSista et al 2008)). And, the temperature sensor elements include the commercially available thermocouples (Chiou et al 2001(Chiou et al , 2002Auroux et al 2003a;Chen et al 2007b;Paik et al 2007), the thermistors (Sista et al 2008;Sugumar et al 2010), and the on-chip film sensors (Bu et al 2003;Wang et al 2005;Polini et al 2010;Sciancalepore et al 2011). The use of integrated film heater and sensor can increase the degree of integration of the microfluidic device, although this may result in an increased cost of fabrication and may require special equipment and increased time for fabrication.…”

Section: Introductionmentioning

confidence: 98%

“…(3) The control of fluid movement in microchannel. The syringe pump is often used to drive the reaction solution to flow through a microchannel to realize the oscillatory-flow PCR (Auroux et al 2003a(Auroux et al ,b, 2004aCheng et al 2005;Wang et al 2005;Chen et al 2007b;Sugumar et al 2010;Polini et al 2010;Sciancalepore et al 2011). The syringe pump is easy to use and simple in operation.…”

Section: Introductionmentioning

confidence: 99%

“…Each substrate material has different properties and therefore different advantages and disadvantages. It should be noted that the commercially available glass capillary has been used to produce the glass-PDMS hybrid chip for the oscillatory-flow PCR (Chen et al 2007b;Polini et al 2010;Sciancalepore et al 2011). Compared with the all-PMDS chip, this hybrid configuration provides greatly reduced water evaporation, ease and low cost of fabrication, and conformal contact with the heater elements (Polini et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…All existing nucleic acid detection methods can be used for offline detection of oscillatory-flow PCR products. Possibly due to cost-effectiveness, the use of intercalators in combination with agarose gel electrophoresis is still the most popular method for detection of oscillatory-flow PCR products (Münchow et al 2005;Wang et al 2005;Cheng et al 2005;Hu et al 2006;Ohashi et al 2007;Chen et al 2007b;Sista et al 2008;Sugumar et al 2010;Polini et al 2010;Sciancalepore et al 2011). Offline product detection is usually time-consuming and labor intensive.…”

Section: Introductionmentioning

confidence: 99%

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Multichannel oscillatory-flow multiplex PCR microfluidics for high-throughput and fast detection of foodborne bacterial pathogens

Zhang

Wang

Xing

2011

Biomed Microdevices

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In the field of continuous-flow PCR, the amplification throughput in a single reaction solution is low and the single-plex PCR is often used. In this work, we reported a flow-based multiplex PCR microfluidic system capable of performing high-throughput and fast DNA amplification for detection of foodborne bacterial pathogens. As a demonstration, the mixture of DNA targets associated with three different foodborne pathogens was included in a single PCR solution. Then, the solution flowed through microchannels incorporated onto three temperature zones in an oscillatory manner. The effect factors of this oscillatory-flow multiplex PCR thermocycling have been demonstrated, including effects of polymerase concentration, cycling times, number of cycles, and DNA template concentration. The experimental results have shown that the oscillatory-flow multiplex PCR, with a volume of only 5 μl, could be completed in about 13 min after 35 cycles (25 cycles) at 100 μl/min (70 μl/min), which is about one-sixth of the time required on the conventional machine (70 min). By using the presently designed DNA sample model, the minimum target concentration that could be detected at 30 μl/min was 9.8 × 10(-2) ng/μl (278-bp, S. enterica), 11.2 × 10(-2) ng/μl (168-bp, E. coli O157: H7), and 2.88 × 10(-2) ng/μl (106-bp, L. monocytogenes), which corresponds to approximately 3.72 × 10(4) copies/μl, 3.58 × 10(4) copies/μl, and 1.79 × 10(4) copies/μl, respectively. This level of speed and sensitivity is comparable to that achievable in most other continuous-flow PCR systems. In addition, the four individual channels were used to achieve multi-target PCR analysis of three different DNA samples from different food sources in parallel, thereby achieving another level of multiplexing.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multichannel oscillatory-flow multiplex PCR microfluidics for high-throughput and fast detection of foodborne bacterial pathogens

Zhang

Wang

Xing

2011

Biomed Microdevices

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“…A moderate amount of a highly viscous liquid with a high boiling point (e.g., mineral oil) flows into the microchannel just before the introduction of the sample solution, which helps increase the pressure of the sample solution in the microchannel. 2,9,11,25 Then, the latter solution follows the flowing mineral oil into the high-temperature region without the generation of air bubbles.…”

Section: Surface Modification and Bubble Reductionmentioning

confidence: 99%

One-heater flow-through polymerase chain reaction device by heat pipes cooling

Chen

Liao

et al. 2015

Biomicrofluidics

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This study describes a novel microfluidic reactor capable of flow-through polymerase chain reactions (PCR). For one-heater PCR devices in previous studies, comprehensive simulations and experiments for the chip geometry and the heater arrangement were usually needed before the fabrication of the device. In order to improve the flexibility of the one-heater PCR device, two heat pipes with one fan are used to create the requisite temperature regions in our device. With the integration of one heater onto the chip, the high temperature required for the denaturation stage can be generated at the chip center. By arranging the heat pipes on the opposite sides of the chip, the low temperature needed for the annealing stage is easy to regulate. Numerical calculations and thermal measurements have shown that the temperature distribution in the five-temperature-region PCR chip would be suitable for DNA amplification. In order to ensure temperature uniformity at specific reaction regions, the Re of the sample flow is less than 1. When the microchannel width increases and then decreases gradually between the denaturation and annealing regions, the extension region located in the enlarged part of the channel can be observed numerically and experimentally. From the simulations, the residence time at the extension region with the enlarged channel is 4.25 times longer than that without an enlarged channel at a flow rate of 2 ll/min. The treated surfaces of the flow-through microchannel are characterized using the water contact angle, while the effects of the hydrophilicity of the treated polydimethylsiloxane (PDMS) microchannels on PCR efficiency are determined using gel electrophoresis. By increasing the hydrophilicity of the channel surface after immersing the PDMS substrates into Tween 20 (20%) or BSA (1 mg/ml) solutions, efficient amplifications of DNA segments were proved to occur in our chip device. To our knowledge, our group is the first to introduce heat pipes into the cooling module that has been designed for a PCR device. The unique architecture utilized in this flow-through PCR device is well applied to a low-cost PCR system. V C 2015 AIP Publishing LLC.

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A Snapshot of Microfluidics in Point‐of‐Care Diagnostics: Multifaceted Integrity with Materials and Sensors

Akceoglu

Saylan

İnci

2021

Adv Materials Technologies

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devices, where engineering, biochemistry, molecular biology, and biomedical technologies are integrated seamlessly with each other. POC devices have great advantages due to their portability, size, accuracy, and low volume of samples. These advantages are fundamentally changing the workflow of health care systems by shortening the turnaround time, accelerating the clinical decisionmaking with early treatment possibilities, and enabling to test individuals at resource-scarce settings, including but not limited to disaster areas, remote settings, or physician office with the limited laboratory access. Ultimately, this crucial direction makes health delivery closer to the patient rather than the provider. [4] In addition, even if the patient is not living in the countryside, the utilization of POC devices at hospitals has exhibited a remarkable reduction in the duration of hospital stay due to the elimination of time-consuming, centralized laboratory testing. [5,6] Since the size and portability are great advantages of POC devices, storage, proper usage, and quality control of tests are still unmet challenges. For instance, external factors, e.g., light, humidity, and temperature, hinder the performance of these tests potentially, and thereby, they need to be controlled comprehensively through regular calibrations, technical service, and maintenance performed by trained and experienced personnel, which potentially increase their cost and complexity, as well as limit their utility at the resourceconstrained settings. [7,8] Microfluidics, on the other hand, denotes a unique opportunity by controlling and manipulating the low volume of liquids (10 -9 to 10 -18 L) precisely; handling samples easily; offering inexpensive and large-scale production with the desired parameters; modulating surface properties and integrating multiple control units; and presenting versatile integrity with different sensing modalities. All these features highlight the potential of microfluidics as a full or integrative unit of POC diagnostic devices. From the production perspective, there are many microfabrication methods, such as replica molding, nanoimprint lithography, SU-8 photoresist, rapid prototyping, microinjection molding, and plasma processing. [9,10] In these platforms, liquids can be controlled in microscale membranes, valves, chambers, reservoirs by mixing or reacting them with each other. Moreover, all these kinds of fashions manipulating the low volume of liquids are creating great opportunities for POC applications, where integration, miniaturization, computerization Over four decades, point-of-care (POC) technologies and their pivotal applications in the biomedical arena have increased irrepressibly and allowed to realize the potential of portable and accurate diagnostic strategies. Today, in the light of these advances, POC systems dominate the medical inventions and bring the diagnostics to the bedside settings, potentially minimizing the workload in the centralized laboratories, as well as remarkably reducing the associated...

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Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow

Cited by 24 publications

References 43 publications

Multichannel oscillatory-flow multiplex PCR microfluidics for high-throughput and fast detection of foodborne bacterial pathogens

Multichannel oscillatory-flow multiplex PCR microfluidics for high-throughput and fast detection of foodborne bacterial pathogens

One-heater flow-through polymerase chain reaction device by heat pipes cooling

A Snapshot of Microfluidics in Point‐of‐Care Diagnostics: Multifaceted Integrity with Materials and Sensors

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