Reductive dechlorination of Î²-hexachlorocyclohexane (Î²-HCH) by a Dehalobacter species in coculture with a Sedimentibacter sp.

Doesburg, Wim van; Eekert, M.H.A. van; Middeldorp, P.J.M.; Balk, Melike; Schraa, G.; Stams, A.J.M.

doi:10.1016/j.femsec.2005.03.003

Cited by 153 publications

(99 citation statements)

References 33 publications

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“…Isolates capable of degrading one or more of the other four HCH isomers under anaerobic conditions include Clostridium rectum (125), Clostridium sphenoides (58,84), Clostridium butyricum, Clostridium pasteurianum (64), Citrobacter freundii (64), Desulfovibrio gigas, Desulfovibrio africanus, Desulfococcus multivorans (21), and a Dehalobacter sp. (178). The ␥-and ␣-isomers are degraded more rapidly than ␦-and ␤-HCH (34,66,85,91,141).…”

Section: Anaerobic Degradationmentioning

confidence: 99%

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

352

338

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SUMMARY Lindane, the γ-isomer of hexachlorocyclohexane (HCH), is a potent insecticide. Purified lindane or unpurified mixtures of this and α-, β-, and δ-isomers of HCH were widely used as commercial insecticides in the last half of the 20th century. Large dumps of unused HCH isomers now constitute a major hazard because of their long residence times in soil and high nontarget toxicities. The major pathway for the aerobic degradation of HCH isomers in soil is the Lin pathway, and variants of this pathway will degrade all four of the HCH isomers although only slowly. Sequence differences in the primary LinA and LinB enzymes in the pathway play a key role in determining their ability to degrade the different isomers. LinA is a dehydrochlorinase, but little is known of its biochemistry. LinB is a hydrolytic dechlorinase that has been heterologously expressed and crystallized, and there is some understanding of the sequence-structure-function relationships underlying its substrate specificity and kinetics, although there are also some significant anomalies. The kinetics of some LinB variants are reported to be slow even for their preferred isomers. It is important to develop a better understanding of the biochemistries of the LinA and LinB variants and to use that knowledge to build better variants, because field trials of some bioremediation strategies based on the Lin pathway have yielded promising results but would not yet achieve economic levels of remediation.

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Section: Anaerobic Degradationmentioning

confidence: 99%

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

352

338

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“…and Sedimentobacter sp. enriched from soil was reported to be able to reductively dechlorinate β-hexachlorocyclohexane 126) .…”

Section: Obligately Dehalorespiring Bacteriamentioning

confidence: 99%

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Hiraishi

2008

Microb. Environ.

103

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A wide variety of haloorganic compounds undergo reductive dehalogenation by certain anaerobic microorganisms. Metabolic reductive dehalogenation is coupled with energy-conserving respiratory electron transport in which a halogenated compound is used as the terminal electron acceptor, the biological process called dehalorespiration or halorespiration. Dehalorespiring bacteria may play important roles in the geochemical cycle with organohalogens in nature and have great promise in their application to the bioremediation of haloorganic contaminants derived from anthropogenic sources. During the past decade, a number of dehalorespiring microorganisms, including a unique group of strictly dehalorespiring bacteria, "Dehalococcoides", have been isolated and characterized at phylogenetic, physiologic, and genetic levels. Also, new perspectives of dehalorespiring bacteria have emerged based on information about genomics and molecular microbial ecology. This review article focuses on up-to-date knowledge of the biodiversity of dehalorespiring bacteria and reductively dehalogenating microbial consortia with special emphasis on those capable of transforming polychlorinated biphenyls and dioxins.

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“…In order to check whether this culture is representative for the population, a denaturing gradient gel electrophoresis (DGGE), according to van Doesburg et al (2005) was carried out for the crude enrichment culture and for culture Uro-30 (Fig. 1).…”

mentioning

confidence: 99%

“…Amplification of the 16S rDNA genes, cloning and sequencing (van Doesburg et al 2005) was done for the crude culture. Total 16S rRNA gene sequences were compared to sequences deposited in publicly accessible databases using NCBI Blast search tool at http://www.ncbi/nlm.nih.gov/blast.…”

mentioning

confidence: 99%

Utilization of hexamethylenetetramine (urotropine) by bacteria and yeasts

Middelhoven

Doesburg

2006

Antonie van Leeuwenhoek

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A slow growing bacterial population able to utilize hexamethylelenetetramine (urotropine) as sole source of carbon, nitrogen and energy was isolated from soil. From this crude enrichment culture two bacteria were isolated and identified as Brevundimonas diminuta and a Phyllobacterium sp. by sequencing of 16S ribosomal DNA. These bacteria also grew on urotropine but at a lower rate than the enrichment culture. Addition of glucose to the latter resulted in growth of some yeasts that overgrew the bacteria. Assimilation of urotropine as sole nitrogen source is very common among yeasts, 46 out of 60 species tested showed this characteristic.

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Reductive dechlorination of Î²-hexachlorocyclohexane (Î²-HCH) by a Dehalobacter species in coculture with a Sedimentibacter sp.

Cited by 153 publications

References 33 publications

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Utilization of hexamethylenetetramine (urotropine) by bacteria and yeasts

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