Reductive transformation of parathion and methyl parathion by Bacillus sp.

Yang, Chao; Dong, Ming; Yuan, Yulan; Huang, Yao; Guo, Xinmin; Qiao, Chuanling

doi:10.1007/s10529-006-9264-7

Cited by 22 publications

(8 citation statements)

References 19 publications

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“…We were not able to highlight a nitroreductase, which is assumed to be induced and involved in the first step of mesotrione degradation as already observed for other nitro pesticides (Yang et al, 2007;Roldan et al, 2008). The involvement of such an enzyme is reinforced by the fact that nitroreductases involved in nitro aromatic compound degradation are tightly linked to proteins revealed in this study as potentially correlated with the mesotrione stress response (NfrA, MhqN, YodC, …; Fig.…”

Section: Nitrogen Metabolismmentioning

confidence: 43%

How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure

Bardot

Besse-Hoggan

Carles

et al. 2015

Environmental Pollution

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Section: Nitrogen Metabolismmentioning

confidence: 43%

How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure

Bardot

Besse-Hoggan

Carles

et al. 2015

Environmental Pollution

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“…Recently, we found a Bacillus sp. strain that transforms parathion and methyl parathion by reducing the nitro group and a nitroreductase involved in the transformation (41). In the present study, we report a transformation pathway that has not been previously described: the reduction of the PAS bond of the thion OPs by Klebsiella sp.…”

Section: Vol 73 2007supporting

confidence: 47%

Cloning of a Novel Aldo-Keto Reductase Gene from Klebsiella sp. Strain F51-1-2 and Its Functional Expression in Escherichia coli

Jiang

Yang

et al. 2007

Appl Environ Microbiol

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A soil bacterium capable of metabolizing organophosphorus compounds by reducing the PAS group in the molecules was taxonomically identified as Klebsiella sp. strain F51-1-2. The gene involved in the reduction of organophosphorus compounds was cloned from this strain by the shotgun technique, and the deduced protein (named AKR5F1) showed homology to members of the aldo-keto reductase (AKR) superfamily. The intact coding region for AKR5F1 was subcloned into vector pET28a and overexpressed in Escherichia coli BL21(DE3). Recombinant His 6 -tagged AKR5F1 was purified in one step using Ni-nitrilotriacetic acid affinity chromatography. Assays for cofactor specificity indicated that reductive transformation of organophosphorus compounds by the recombinant AKR5F1 specifically required NADH. The kinetic constants of the purified recombinant AKR5F1 toward six thion organophosphorus compounds were determined. For example, the K m and k cat values of reductive transformation of malathion by the purified recombinant AKR5F1 are 269.5 ؎ 47.0 ⌴ and 25.7 ؎ 1.7 min ؊1 , respectively. Furthermore, the reductive transformation of organophosphorus compounds can be largely explained by structural modeling.Synthetic organophosphorus (OP) compounds are widely used in agriculture to control major insect pests. These compounds have been implicated in several nerve and muscular diseases in human beings. Microbial degradation of OP pesticide has become the focus of many studies because it is economical and effective (30). The most widely studied bacterial enzyme for OP detoxification is the OP hydrolase (OPH). OPH has been isolated from several bacteria (23,29), and the OPH from Pseudomonas diminuta has the widest range of substrate specificity (6). A similar enzyme, OPH A (OPDA), has been isolated from Agrobacterium radiobacter and was found to have 90% homology to OPH at the amino acid level (16). However, the rates of hydrolysis by OPH differ dramatically for members of the OP compound family, ranging from the diffusion-controlled limit for hydrolysis of paraoxon to several orders of magnitude slower for malathion, chlorpyrifos, and VX (6).Various attempts have been made to isolate malathion-degrading microorganisms. Matsumura et al. isolated a malathiondegrading fungus, Trichoderma viride, and a bacterium, Pseudomonas sp., from contaminated soil, which rapidly degrades malathion through carboxylesteratic hydrolysis as well as desmethylation processes (22). Lewis et al. isolated a fungus, Aspergillus oryzae, from a freshwater pond, which transforms malathion to ␤-malathion monoacid and malathion dicarboxylic acid, indicating the presence of carboxylesteratic hydrolysis activity (19). Degradation of malathion by other bacterial species was also reported (11). Although many studies have been carried out on the microbial degradation of malathion, there is a lack of information on the genetic and enzymatic aspects in the degradation of malathion.The aldo-keto reductases (AKRs) are a growing superfamily of approximately 120 enzymes, currently...

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“…Sin embargo, Liu et al (2007) reportaron que el pnp no se forma durante la biorremediación de mp, lo que sugiere que A. radioresistens puede degradar mp por otras vías. Yang et al (2007) demostraron que la degradación microbiana de paratión se puede producir a través de tres vías: en primer lugar, la formación de p-nitrofenol por la hidrólisis del enlace fosfotriéster; en segundo lugar, la reducción del grupo nitro en condiciones bajas de oxígeno y, después, la hidrólisis para producir p-aminofenol; y, en tercer lugar, la conversión del paratión en paraoxón antes de la hidrólisis del enlace fosfotriéster.…”

Section: Paratión -Metil Paratiónunclassified

Biorremediación de organofosforados por hongos y bacterias en suelos agrícolas: revisión sistemática

Hernández-Ruiz¹

2016

Corpoica Cienc. Tecnol. Agropecuaria

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<p>Los organofosforados son un tipo de plaguicidas ampliamente utilizados en el sector agrícola para el control de plagas. Dado que estos son compuestos químicos altamente tóxicos, su uso excesivo ha causado gran deterioro en los suelos cultivables, así como graves daños para los ecosistemas y la salud humana. La biorremediación surge como una alternativa para transformar los plaguicidas en compuestos más simples y poco contaminantes mediante el uso del potencial metabólico de los microorganismos. Por lo anterior, el objetivo de esta investigación fue describir los hongos y bacterias involucrados en la biorremediación de los principales plaguicidas organofosforados empleados en suelos agrícolas por medio de una revisión sistemática de la literatura científica, con el fin de aportar información útil para la realización de estudios posteriores. Se obtuvo información científica de las bases de datos ScienceDirect y Springer Link, y también información no indexada del buscador Google Scholar. Se encontró que el organofosforado que más se ha estudiado es el clorpirifós (categoría toxicológica III) y los microorganismos que más se utilizan como biorremediadores de organofosforados son los géneros Serratia, Bacillus y Pseudomonas. Se concluye que el éxito de la biorremediación depende de la capacidad competitiva de los microorganismos, de la biodisponibilidad y la concentración del organofosforado, del pH, la temperatura y el tipo de suelo, así como de la presencia de suplementos nutricionales y de la concentración alta del inóculo.</p>

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Reductive transformation of parathion and methyl parathion by Bacillus sp.

Cited by 22 publications

References 19 publications

How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure

How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure

Cloning of a Novel Aldo-Keto Reductase Gene from Klebsiella sp. Strain F51-1-2 and Its Functional Expression in Escherichia coli

Biorremediación de organofosforados por hongos y bacterias en suelos agrícolas: revisión sistemática

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