2019
DOI: 10.1128/iai.00894-18
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Redundant and Distinct Roles of Secreted Protein Eap and Cell Wall-Anchored Protein SasG in Biofilm Formation and Pathogenicity of Staphylococcus aureus

Abstract: Chronic and fatal infections caused by Staphylococcus aureus are sometimes associated with biofilm formation. Secreted proteins and cell wall-anchored proteins (CWAPs) are important for the development of polysaccharide-independent biofilms, but functional relationships between these proteins are unclear. In the present study, we report the roles of the extracellular adherence protein Eap and the surface CWAP SasG in S. aureus MR23, a clinical methicillin-resistant isolate that forms a robust protein-dependent… Show more

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Cited by 27 publications
(22 citation statements)
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“…Biofilm formation and quantification were performed as previously reported 31 , 44 46 . The bacterial strains were grown overnight at 37 °C in BHI medium.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Biofilm formation and quantification were performed as previously reported 31 , 44 46 . The bacterial strains were grown overnight at 37 °C in BHI medium.…”
Section: Methodsmentioning
confidence: 99%
“…A plasmid for the complementation of icaA deletion was constructed using E. coli – S. aureus shuttle vector pLC1, as previously described 46 , 48 (Table 1 ). Briefly, pLC1 was linearized by inverse PCR using KOD Plus (Toyobo) and the primers pLC1-F and pLC1-R (Supplementary Table 1 ).…”
Section: Methodsmentioning
confidence: 99%
“…Loss of sortase (sarA), which anchors LPXTG-containing proteins to peptidoglycan, reduced the MRSA biofilm phenotype [700]. Other proteins involved in ica-independent biofilm formation include Staphylococcus aureus surface proteins C and G (SasC and SasG), extracellular adherence protein (Eap), biofilm-associated protein (Bap), fibronectin-binding proteins (FnBPs), clumping factor B (ClfB), and Staphylococcus protein A (Spa) [704][705][706][707][708][709].…”
Section: Biofilm Formation By Staphylococcus Speciesmentioning
confidence: 99%
“…The role of proteins in biofilm formation of staphylococci is currently most widely studied in Staphylococcus aureus and Staphylococcus epidermidis . Next to secreted proteins, such as the 18 kDa small basic protein (Sbp) (Decker et al, 2015) and the extracellular adherence protein (Eap) (Yonemoto et al, 2019), cell wall‐anchored proteins play a major role in the biofilm formation process. They either mediate primary attachment to surfaces and/or cell accumulation at later stages of the biofilm maturation phase (Foster, 2019; Speziale et al, 2014).…”
Section: Introductionmentioning
confidence: 99%