Reevaluation of
            <i>Streptococcus bovis</i>
            Endocarditis Cases from 1975 to 1985 by 16S Ribosomal DNA Sequence Analysis

Herrero, I.; Rouse, Mark S.; Piper, Kerryl E.; Alyaseen, Samer A.; Steckelberg, James M.; Patel, Robin

doi:10.1128/jcm.40.10.3848-3850.2002

Cited by 52 publications

(28 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies have reported the association of Streptococcus (St.) bovis endocarditis with colon cancer. [1][2][3][4] However, this association has not been investigated. Streptococcus bovis has been divided by biotype into three types, I, II/1, and II/2, although it has recently been reclassifi ed both genetically and biochemically.…”

Section: Introductionmentioning

confidence: 94%

Association of tannase-producing Staphylococcus lugdunensis with colon cancer and characterization of a novel tannase gene

et al. 2007

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 94%

Association of tannase-producing Staphylococcus lugdunensis with colon cancer and characterization of a novel tannase gene

et al. 2007

View full text Add to dashboard Cite

show abstract

“…[3][4][5][6][7][8][9][10] This bacterium is responsible for a growing number of infective endocarditis (IE) and septicemia cases in elderly and immunocompromised individuals. [11][12][13][14][15][16] Firstly isolated from Koala feces and named for its capacity to degrade host tannins, 17 S. gallolyticus is a common inhabitant of the rumen flora of herbivores. This species has also been found in the gastrointestinal (GI) tract of a wide spectrum of animals, [17][18][19][20][21] including marsupials, mammals and birds.…”

Section: Addendummentioning

confidence: 99%

The Pil3 pilus ofStreptococcus gallolyticusbinds to intestinal mucins and to fibrinogen

et al. 2016

View full text Add to dashboard Cite

Streptococcus gallolyticus is a commensal bacterium responsible for infectious endocarditis in the elderly, which has frequently been associated with colonic carcinoma. Whether this species is a cause or a consequence of colorectal cancer remains unknown. We recently demonstrated that S. gallolyticus Pil3 pilus is required for adhesion to colonic mucus and for colonization of mouse distal colon. We show here that Pil3 pilus binds equally well to human colonic mucins derived from HT29-MTX cells and to human stomach mucins from healthy donors. In addition, we have found that Pil3 also binds to human fibrinogen, which expands the repertoire of Pil3 host ligands.

show abstract

“…Subsequently, wholemetagenome shotgun sequencing showed that most samples were dominated by Streptococcus infantarius, a species that had been identified previously in other African dairy products (29,30). Of concern, S. infantarius has been linked to several human diseases, including bacteremia (31), endocarditis (32), and colon cancer (33). Aside from S. infantarius, two other potentially pathogenic species, namely, Escherichia coli and Klebsiella pneumoniae, were identified in a subset of samples.…”

Section: Discussionmentioning

confidence: 99%

Strain-Level Metagenomic Analysis of the Fermented Dairy Beverage Nunu Highlights Potential Food Safety Risks

Walsh

Crispie

Daari

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

The rapid detection of pathogenic strains in food products is essential for the prevention of disease outbreaks. It has already been demonstrated that whole-metagenome shotgun sequencing can be used to detect pathogens in food but, until recently, strain-level detection of pathogens has relied on wholemetagenome assembly, which is a computationally demanding process. Here we demonstrated that three short-read-alignment-based methods, i.e., MetaMLST, PanPhlAn, and StrainPhlAn, could accurately and rapidly identify pathogenic strains in spinach metagenomes that had been intentionally spiked with Shiga toxin-producing Escherichia coli in a previous study. Subsequently, we employed the methods, in combination with other metagenomics approaches, to assess the safety of nunu, a traditional Ghanaian fermented milk product that is produced by the spontaneous fermentation of raw cow milk. We showed that nunu samples were frequently contaminated with bacteria associated with the bovine gut and, worryingly, we detected putatively pathogenic E. coli and Klebsiella pneumoniae strains in a subset of nunu samples. Ultimately, our work establishes that short-read-alignmentbased bioinformatics approaches are suitable food safety tools, and we describe a real-life example of their utilization.IMPORTANCE Foodborne pathogens are responsible for millions of illnesses each year. Here we demonstrate that short-read-alignment-based bioinformatics tools can accurately and rapidly detect pathogenic strains in food products by using shotgun metagenomics data. The methods used here are considerably faster than both traditional culturing methods and alternative bioinformatics approaches that rely on metagenome assembly; therefore, they can potentially be used for more high-throughput food safety testing. Overall, our results suggest that wholemetagenome sequencing can be used as a practical food safety tool to prevent diseases or to link outbreaks to specific food products.KEYWORDS fermentation, food-borne pathogens, metagenomics I n recent years, high-throughput sequencing (HTS) has become an important tool in food microbiology (1). HTS enables in-depth characterization of food-related microbial isolates, via whole-genome sequencing (WGS), and it facilitates cultureindependent analysis of mixed microbial communities in foods, via metagenomic sequencing.WGS has provided invaluable insights into the genetics of starter cultures (2, 3), and it is routinely used in epidemiology to identify outbreak-associated foodborne pathogens isolated from clinical samples, through comparison of the single-nucleotide polymorphism (SNP) profiles of outbreak strain genomes versus nonoutbreak strain

show abstract

Reevaluation of Streptococcus bovis Endocarditis Cases from 1975 to 1985 by 16S Ribosomal DNA Sequence Analysis

Cited by 52 publications

References 16 publications

Association of tannase-producing Staphylococcus lugdunensis with colon cancer and characterization of a novel tannase gene

Association of tannase-producing Staphylococcus lugdunensis with colon cancer and characterization of a novel tannase gene

The Pil3 pilus ofStreptococcus gallolyticusbinds to intestinal mucins and to fibrinogen

Strain-Level Metagenomic Analysis of the Fermented Dairy Beverage Nunu Highlights Potential Food Safety Risks

Contact Info

Product

Resources

About